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Construction and identification of lentiviral RNA interference vector of rat leptin receptor gene

Frontiers of Medicine 2009年第1期3卷 57-60页

作者： Zhengjuan LIU Jie BIAN Yuchuan WANG Yongli ZHAO Dong YAN Xiaoxia WANG Department of Pediatrics the Second Affiliated HospitalDalian Medical UniversityDalian 116027China

leptin resistance is a main mechanism of acquired childhood obesity,and the suppression of long form of leptin receptor(OBRb)gene expression in diet-induced obese rats indicates that the down-regulation of OBRb gene expression plays a pivotal role in the mechanism of leptin *** aim of the present study was to construct the lentiviral RNA interference(RNAi)vector of rat OBRb gene and evaluate the effects of siRNA on silencing OBRb gene *** target sequence of siRNA-OBRb was designed,and the com-plementary DNA containing both sense and antisense oligonucleotides was *** phosphorylation and annealing,these double-stranded DNA was cloned to pRNA-lentivector-VGFP to construct pRNA-Lenti-OBRb-VGFP recombinants with U6-containing promoter,target sequence and Poly III ***,the products were conﬁrmed by electrophoresis and sequencing analy-sis,and the effects of RNAi on reducing gene expression were further conﬁrmed by real-time polymerase chain reaction in transfected rat glioma cells expressing *** target sequence of siRNA-OBRb was successfully cloned to pRNA-lentivector-VGFP,and the RNAi protocol speciﬁcally reduced the expression of OBRb mRNA by approximately 80%compared with controls in transfected rat glioma *** successful construction of rat lentivirus vectors expressing OBRb-speciﬁc shRNA may be useful for further investigation in vivo.

关键词： receptors,leptin RNA interference lentivirus vector

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Influence of catgut implantation at acupoints on leptin and insulin resistance in simple obesity rats

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Journal of Traditional Chinese Medicine 2012年第3期32卷 477-481页

作者： Runhu Yan Xiaoming Liu Jie Bai Jiang Yu Jinsong Gu College of Cosmetology Medicine of DalianMedical University Research institute of Integrated Tradi-tional and Western Medicine of Dalian Medical University Dalian 116044 China Dermatology of The First Affiliated Hospitalof Dalian Medical University Dalian 116011 China Jiang Yu Jinsong Gu College of Cosmetology Medicine of Dalian Medical University Dalian 116044 China

OBJECTIVES:To investigate the influence of catgut implantation at acupoints on leptin resistance(LR) and insulin resistance(IR) in the simple obesity ***:Rats were made obese with high-fat diets,after which surgical catgut was implanted at Zusanli(ST 36) and Neiting(ST 44) acupoints once a week for 4 weeks(implantation group).Rats from the implantation group were compared with normal rats and unoperated obese rats(control group).Gene expression of the leptin receptor(OB-R) was evaluated using RT-PCR and northern *** and hypothalamus leptin and insulin(INS) levels were determined by *** weight,Lee's index,body fat,serum and hypothalamus leptin and INS levels,and hypothalamic OB-R gene expression were determined before and after ***:Body weights,Lee's index,body fat,and serum leptin and INS levels were significantly higher in obese than in normal *** leptin and INS levels and OB-R gene expression were significantly lower in obese *** implantation at acupoint promoted weight loss and decreased serum leptin and INS *** leptin and INS levels and OB-R gene expression increased ***:Catgut implantation at acupoint adjusts central and peripheral leptin and promotes hypothalamic OB-R gene *** may be an important method for regulation of LR,IR and abnormal endocrinology and metabolism.

关键词： Thread-embedding acupoint ligation Obesity leptin resistance Insulin resistance receptors,leptin Blood-cerebral barrier

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