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Application of decellularization-recellularization technique in plastic and reconstructive surgery

Chinese Medical Journal 2023年第17期136卷 2017-2027页

作者： Yujia Shang Guanhuier Wang Yonghuan Zhen Na Liu Fangfei Nie Zhenmin Zhao Hua Li Yang An Department of Plastic Surgery Peking University Third HospitalHaidian DistrictBeijing 100191China Wuya College of Innovation Key Laboratory of Structure-Based Drug Design&DiscoveryMinistry of EducationShenyang Pharmaceutical UniversityShenyangLiaoning 110016China

In the field of plastic and reconstructive surgery,the loss of organs or tissues caused by diseases or injuries has resulted in challenges,such as donor shortage and immunosuppression.In recent years,with the development of regenerative medicine,the decellularization-recellularization strategy seems to be a promising and attractive method to resolve these difficulties.The decellularized extracellular matrix contains no cells and genetic materials,while retaining the complex ultrastructure,and it can be used as a scaffold for cell seeding and subsequent transplantation,thereby promoting the regeneration of diseased or damaged tissues and organs.This review provided an overview of decellularization-recellularization technique,and mainly concentrated on the application of decellularization-recellularization technique in the field of plastic and reconstructive surgery,including the remodeling of skin,nose,ears,face,and limbs.Finally,we proposed the challenges in and the direction of future development of decellularization-recellularization technique in plastic surgery.

关键词： Decellularization recellularization Extracellular matrix Plastic surgery Tissue regeneration

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Decellularized extracellular matrix scaffolds:Recent trends and emerging strategies in tissue engineering

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Bioactive Materials 2022年第4期7卷 15-31页

作者： Xuewei Zhang Xi Chen Hua Hong Rubei Hu Jiashang Liu Changsheng Liu Key Laboratory for Ultrafine Materials of Ministry of Education Frontiers Science Center for Materiobiology and Dynamic ChemistryEngineering Research Center for Biomaterials of Ministry of EducationSchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai200237PR China

The application of scaffolding materials is believed to hold enormous potential for tissue regeneration.Despite the widespread application and rapid advance of several tissue-engineered scaffolds such as natural and synthetic polymer-based scaffolds,they have limited repair capacity due to the difficulties in overcoming the immunogenicity,simulating in-vivo microenvironment,and performing mechanical or biochemical properties similar to native organs/tissues.Fortunately,the emergence of decellularized extracellular matrix(dECM)scaffolds provides an attractive way to overcome these hurdles,which mimic an optimal non-immune environment with native three-dimensional structures and various bioactive components.The consequent cell-seeded construct based on dECM scaffolds,especially stem cell-recellularized construct,is considered an ideal choice for regenerating functional organs/tissues.Herein,we review recent developments in dECM scaffolds and put forward perspectives accordingly,with particular focus on the concept and fabrication of decellularized scaffolds,as well as the application of decellularized scaffolds and their combinations with stem cells(recellularized scaffolds)in tissue engineering,including skin,bone,nerve,heart,along with lung,liver and kidney.

关键词： Decellularization ECM 3D scaffolds Tissue regeneration recellularization

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A Novel Low Air Pressure-Assisted Approach for the Construction of Cells-Decellularized Tendon Scaffold Complex

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Current Medical Science 2022年第3期42卷 569-576页

作者： Xin HUANG Zheng-tao LV Peng CHENG An-min CHEN Department of Orthopedics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Objective The goal of this study was to develop a decellularized tendon scaffold(DTS)and repopulate it with adipose-derived stem cells(ADSCs)assisted by low air pressure(LP).Methods The porcine superficial flexor tendons were processed into the DTSs using a combination of physical,chemical,and enzymatic treatments.The effectiveness of decellularization was verified by histological analysis and DNA quantification.The properties of the DTSs were evaluated by quantitative analysis of biochemical characterization,porosimetry,in vitro biocompatibility assessment,and biomechanical testing.Subsequently,the ADSCs-DTS complexes were constructed via cell injection assisted by LP or under atmospheric pressure.The differences in cell distribution,biomechanical properties,and the total DNA content were compared by histological analysis,biomechanical testing,and DNA quantification,respectively.Results Histological analysis confirmed that no cells or condensed nuclear materials were retained within the DTSs with widened interfibrillar space.The decellularization treatment resulted in a significant decrease in the content of DNA and glycosaminoglycans,and a significant increase in the porosity.The DTSs were cytocompatible in vitro and did not show reduced collagen content and inferior biomechanical properties compared with the fresh-frozen tendons.The assistance of LP promoted the broader distribution of cells into the adjacent interfibrillar space and cell proliferation in DTSs.The biomechanical properties of the scaffolds were not significantly affected by the recellularization treatments.Conclusion A novel LP-assisted approach for the construction of cells-DTS complex was established,which could be a methodological foundation for further bioreactor and in vitro studies.

关键词： tendon decellularization adipose-derived stem cells recellularization low air pressure tissue engineering

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Generation of functional organs from stem cells

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Cell Regeneration 2013年第1期2卷 1-6页

作者： Yunying Liu Ru Yang Zuping He Wei-Qiang Gao State Key Laboratory of Oncogenes and Related Genes Stem Cell Research CenterRenji HospitalShanghai Jiaotong University School of MedicineShanghaiChina Med-X Research Institute Shanghai Jiaotong UniversityShanghai 200127China

We are now well entering the exciting era of stem cells.Potential stem cell therapy holds great promise for the treatment of many diseases such as stroke,traumatic brain injury,Alzheimer’s disease,Parkinson’s disease,amyotrophic lateral-sclerosis,myocardial infarction,muscular dystrophy,diabetes,and etc..It is generally believed that transplantation of specific stem cells into the injured tissue to replace the lost cells is an effective way to repair the tissue.In fact,organ transplantation has been successfully practiced in clinics for liver or kidney failure.However,the severe shortage of donor organs has been a major obstacle for the expansion of organ transplantation programs.Toward that direction,generation of transplantable organs using stem cells is a desirable approach for organ replacement and would be of great interest for both basic and clinical scientists.Here we review recent progress in the field of organ generation using various methods including single adult tissue stem cells,a blastocyst complementation system,tissue decellularization/recellularization and a combination of stem cells and tissue engineering.

关键词： Stem cells Functional organs Blastocyst complementation Decellularization recellularization Tissue engineering

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