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Profiling of the assembly of reca nucleofilaments implies a potential target for environmental factors to disturb DNA repair

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Journal of Environmental Sciences 2021年第4期33卷 283-290页

作者： Zheng Yuan Fangzhi Yu Dapeng Zhang Hailin Wang Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China Institute of Environment and Health Jianghan UniversityWuhan 430056China Institute of Environment and Health Hangzhou Institute for Advanced StudyUCASHangzhou 430056China

Double-strand breaks(DSBs),one class of the most harmful DNA damage forms that bring elevated health risks,need to be repaired timely and ***,an increasing number of environmental pollutants have been identified to impair DSB repair from various *** previous work indicated that the formation of unsaturated Rec A nucleofilaments plays an essential role in homology recombination(HR) pathway which can accurately repair *** this study,by developing a benzonase cutting protection assay and combining it with traditional electrophoretic mobility shift assay(EMSA) analysis,we further investigated the assembly patterns of four Rec A mutants that display differential DSB repair ability and ATPase *** observed that the mutants(G204S and S69G) possessing both ATP hydrolysis and DSB repair activities form unsaturated nucleofilaments similar to that formed by the wild type Rec A,whereas the other two ATP hydrolysis-deficient mutants(K72R and E96D) that fail to mediate HR form more compacted nucleofilaments in the presence of *** results establish a coupling of ATPase activity and effective DSB repair ability via the assembly status of Rec A *** linkage provides a potential target for environmental factors to disturb the essential HR pathway for DSB repair by suppressing the ATPase activity and altering the assembly pattern of nucleofilaments.

关键词： DNA double-strand breaks(DSBs) reca assembly Homology recombination(HR) ATP hydrolysis DNA damage repair Environmental health

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