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Ginsenoside Rk1 suppresses pro-inflammatory responses in lipopolysaccharide-stimulated raw264.7 cells by inhibiting the Jak2/Stat3 pathway

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Chinese Journal of Natural Medicines 2017年第10期15卷 751-757页

作者： YU Qian ZENG Ke-Wu MA Xiao-Li JIANG Yong TU Peng-Fei WANG Xue-Mei Research Studio of Integration of Traditional and Western Medicine First HospitalPeking UniversityBeijing 100034China State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China

The saponin ginsenoside Rk1 is a major compound isolated from *** Rk1 has been reported to have anti-inflammatory and anti-tumor properties and to be involved in the regulation of ***,the effect and mechanism of anti-inflammatory action of ginsenoside Rk1 has not been fully *** investigated whether ginsenoside Rk1 could suppress the inflammatory response in lipopolysaccharide-stimulated raw264.7 macrophages and to explore its mechanism of the ***264.7 cells were treated with LPS(1 μg×mL^(–1))in the absence or the presence of Ginsenoside Rk1(10,20,and 40 μmol×L^(–1)).Then the inflammatory factors were tested with Griess reagents,ELISA,and *** proteins were analyzed by Western *** Rk1 inhibited lipopolysaccharide-induced expression of nitric oxide(NO),interleukin(IL)-6,IL-1β,tumor necrosis factor(TNF)-α,and monocyte chemotactic protein(MCP)-*** Rk1 inhibited the lipopolysaccharide-stimulated phosphorylation of NF-κB and janus kinase(Jak)2 and signal transducer and activator of transcription(Stat)3 at Ser727 and *** data suggested that ginsenoside Rk1 could inhibit expression of inflammatory mediators and suppress inflammation further by blocking activation of NF-κB and the Jak2/Stat3 pathway in LPS-stimulated raw264.7 cells.

关键词： Inflammation raw264.7 cells Ginsenoside Rkl Jak2/Stat3 NF-xB

Pro-inflammatory effects of a litchi protein extract in murine raw264.7 macrophages

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Horticulture Research 2016年第1期3卷 219-225页

作者： Xiaoli Wang Xiaorong Hu Huiqing Yan Zhaocheng Ma Xiuxin Deng Key Laboratory of Horticultural Plant Biology College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan430070China Department of Cardiology Renmin Hospital of Wuhan UniversityWuhan430070China

It has been observed that the consumption of litchi often causes symptoms characterized by itching or sore throat,gum swelling,oral cavity ulcers and even fever and inflammation,which significantly impair the quality of life of a large *** the raw264.7 cell line,a step-by-step strategy was used to screen for the components in litchi fruits that elicited adverse *** adverse reaction fractions were identified by mass spectrometry and analyzed using the SMART program,and a sequence alignment of the homologous proteins was *** tests were used to determine the cytotoxicity of a litchi protein extract in raw264.7 macrophages,and real-time PCR was applied to analyze the expression of inflammatory genes in the raw264.7 cells treated with lipopolysaccharide or the litchi protein *** results showed that the litchi water-soluble protein extract could increase the production of the pro-inflammatory mediators IL-1β,iNOS and COX-2,and the anti-inflammatory mediator HO-1 in the raw264.7 cell *** 14-3-3-like proteins GF14 lambda,GF14 omega and GF14 upsilon were likely the candidate proteins that caused the adverse effects.

关键词： raw264.7 inflammatory inflammation

鸡蛋中卵黄高磷蛋白对raw264.7细胞生长及分化的影响

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中国食品学报 2020年第8期20卷 29-40页

作者：介怡琳李晓云纪胜男赵前程马美湖黄茜华中农业大学食品科技学院蛋品加工技术国家地方联合工程中心农业农村部蛋品加工重点实验室武汉430070

研究了从鸡蛋黄中提取的黄高磷蛋白(PV)(0,50,100,200μg/mL和500μg/mL)对raw264.7细胞增殖、周期、凋亡的影响,结果发现低浓度PV对raw264.7细胞有促进增殖和抑制凋亡的作用,当PV质量浓度100μg/mL时效果最好。采用CCK-8法表征增殖活力... 详细信息

研究了从鸡蛋黄中提取的黄高磷蛋白(PV)(0,50,100,200μg/mL和500μg/mL)对raw264.7细胞增殖、周期、凋亡的影响,结果发现低浓度PV对raw264.7细胞有促进增殖和抑制凋亡的作用,当PV质量浓度100μg/mL时效果最好。采用CCK-8法表征增殖活力,结果发现PV质量浓度为100μg/mL时增殖活力高出对照组64%~76%。分析PV作用24 h和48 h后对细胞周期的影响,不同浓度的PV对细胞增殖均有促进作用,与对照组相比低质量浓度PV(50,100、200μg/mL)处理细胞后S期细胞的百分比含量、增殖指数PI值大。凋亡结果表明,50,100,200μg/mL PV组凋亡率显著低于空白对照组,500μg/mL时凋亡率高于空白组。另外,在抗酒石酸酸性磷酸酶(TRAP)染色细胞分化形态学及TRAP活性方面,不同浓度的PV均可显著减少破骨细胞数量,降低TRAP活性(Praw264.7细胞生长而是通过显著降低TRAP的活性来抑制raw264.7细胞分化成为破骨细胞。

关键词：卵黄高磷蛋白 raw264.7 细胞增殖细胞周期细胞凋亡细胞分化

raw264.7细胞中姜黄素抗动脉粥样硬化作用机制的蛋白质组学研究

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中国中药杂志 2011年第9期36卷 1207-1211页

作者：卢德赵林韬琦沃兴德王萍儿周云杨贞浙江中医药大学生命科学学院浙江杭州310053

目的:应用凝胶内差异显示电泳技术和质谱技术研究姜黄素抗动脉粥样硬化的作用机制。方法:raw264.7细胞和经过25μmol.L-1姜黄素处理的raw264.7细胞蛋白质经荧光染料Cy3,Cy5标记,与等量Cy2标记的内标混合后在同一胶中进行电泳分离,经不... 详细信息

目的:应用凝胶内差异显示电泳技术和质谱技术研究姜黄素抗动脉粥样硬化的作用机制。方法:raw264.7细胞和经过25μmol.L-1姜黄素处理的raw264.7细胞蛋白质经荧光染料Cy3,Cy5标记,与等量Cy2标记的内标混合后在同一胶中进行电泳分离,经不同光激发后扫描得到不同样品的蛋白质组图谱。经DeCyder6.5软件进行差异蛋白质组分析,并对差异蛋白质进行质谱鉴定。结果:ATP合成酶,MHC classⅡ,肌球蛋白轻链,细胞色素b5表达量增加,而磷酸二酯酶4D,elF-3,Hnrpf蛋白,波形蛋白,核仁磷蛋白1,Ran结合蛋白1表达量降低。结论:姜黄素抗动脉粥样硬化作用的机制十分复杂,是其增强细胞抗炎、抗氧化能力以及抑制胆固醇转运,降低细胞内胆固醇积累等因素共同作用的结果。另外,姜黄素可能通过调节细胞的分化及凋亡起到抗肿瘤的作用。

关键词：姜黄素动脉粥样硬化蛋白质组 raw264.7

金银花黄酮对过氧化氢诱导raw264.7巨噬细胞损伤的保护作用

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中国食品学报 2019年第5期19卷 18-25页

作者：罗磊张冰洁韦倩倩樊金玲马丽苹关宁宁朱文学河南科技大学食品与生物工程学院河南省农产品干燥装备工程技术研究中心河南洛阳471023

探讨金银花黄酮对raw264.7巨噬细胞抗氧化活性的影响。通过过氧化氢诱导raw264.7细胞损伤,建立细胞氧化损伤模型,研究金银花黄酮低、中、高剂量组(62.5,125,250 mg/mL)对损伤细胞的保护作用。研究结果表明:质量浓度62.5～250 mg/mL范围... 详细信息

探讨金银花黄酮对raw264.7巨噬细胞抗氧化活性的影响。通过过氧化氢诱导raw264.7细胞损伤,建立细胞氧化损伤模型,研究金银花黄酮低、中、高剂量组(62.5,125,250 mg/mL)对损伤细胞的保护作用。研究结果表明:质量浓度62.5～250 mg/mL范围的金银花黄酮可显著促进raw264.7细胞的增殖(Praw264.7细胞的增殖,并且对过氧化氢诱导的raw264.7细胞损伤有良好的保护作用,存在剂量依赖效应。本研究为金银花功能性食品及保健品的开发提供理论依据。

关键词：金银花黄酮 raw264.7 过氧化氢抗氧化

raw264.7 Cells Secrete Fibroblast Growth Stimulating Activity after Differentiation to Macrophages by Stimulation with Lipopolysaccharide

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CellBio 2014年第3期3卷 87-95页

作者： Jing-Yang Lai Chung-Li Shu Kazuhiro Morishita Tomonaga Ichikawa Yasuhisa Fukui Division of Tumor and Cellular Biochemistry Department of Medical Sciences Faculty of Medicine University of Miyazaki Miyazaki Japan Institute of Cellular and System Medicine National Health Research Institute Miaoli County Taiwan

raw264.7 cells are monocytic cells that can differentiate to activated macrophages after lipopoly-saccharide (LPS) stimulation. Here, we analyzed the factors secreted by raw264.7 cells in response to LPS. The culture media of LPS-treated raw264.7 cells was able to stimulate growth in MEF1F2 and NIH3T3 mouse fibroblast cell lines. We identified five secreted and LPS-induced chemokines, CCL2, CCL5, CCL12, CxCL2, and CxCL10, by microarray analysis and tested their stimulatory activity. We used commercially available bacterially expressed proteins, and found only CCL12, CxCL2 and CxCL10 stimulated growth in MEF1F2 and NIH3T3 cells. The saturation density of the cells was also increased. They were not able to stimulate growth in v-Src transformed MEF1F2 or SWAP-70 transformed NIH3T3 cells. We examined signaling pathways activated by these three factors. We found that ERK and p38 MAP kinase were activated and were required for the activity to stimulate the cell growth. Other pathways including phosophatidylinositol-3 kinase (PI3K), NFκB pathways were not activated. These results suggest that raw264.7 cells secretes growth stimulation factors for fibroblasts when differentiated to macrophages implicating that fast growth of them is related to inflamation although the reason is still unclear.

关键词： raw264.7 Cells Chemokine Fibroblasts Macrophage

稳定表达结核分枝杆菌ESAT-6蛋白的raw264.7细胞系的建立

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生物工程学报 2011年第9期27卷 1390-1396页

作者：李浩殷瑛董大勇张军付玲蔡晨光王猛徐俊杰陈薇军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室北京100071

为研究结核分枝杆菌Mycobacterium tuberculosis分泌蛋白ESAT-6(Early secreted antigenic target of 6 kDa)对巨噬细胞相关功能的影响,将正确构建的重组质粒pEGFP-C1-ESAT-6和空载体pEGFP-C1以脂质体介导的方法转染至小鼠巨噬细胞raw264.7中,经过G418筛选后建立稳定表达EGFP-ESAT6融合蛋白以及EGFP的细胞系,并通过RT-PCR、荧光显微镜及Western blotting方法,在基因和蛋白两个水平对所建立的稳转细胞系进行鉴定。结果证实EGFP-ESAT6融合基因成功整合入raw264.7细胞基因组并能够稳定表达,为后续的ESAT-6调控巨噬细胞机理研究提供了平台。

关键词： ESAT-6 raw264.7 稳定转染表达

Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on raw264.7 macrophages

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第1期23卷 84-88页

作者： Feifei LI Kehai LIU Kewu LIU College of Food Science and Technology Shanghai Ocean UniversityShanghai201306China Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation Shanghai201306China Heilongjiang Forest By-product and Speciality Institute Mudanjiang157011China

This study aimed to characterize the chemical composition of a new sulfated polysaccharide from the red alga Gracilaria chouae and evaluate its activation effects on raw264.7 *** showed that the obtained *** polysacch... 详细信息

关键词： activation raw264.7 characterization

草苁蓉多糖对脂多糖诱导的raw264.7巨噬细胞炎症反应的影响

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中国药学杂志 2021年第18期56卷 1479-1485页

作者：刘莉园张钊葛乃嘉全吉淑延边大学医学院吉林延吉133002

目的探讨草苁蓉多糖(Boschniakia rossica polysaccharides, BRPS)对脂多糖(lipopolysaccharide, LPS)诱导的raw264.7巨噬细胞炎症反应的影响。方法体外利用LPS刺激raw264.7细胞,建立巨噬细胞炎症模型。将巨噬细胞随机分为正常组、模型... 详细信息

目的探讨草苁蓉多糖(Boschniakia rossica polysaccharides, BRPS)对脂多糖(lipopolysaccharide, LPS)诱导的raw264.7巨噬细胞炎症反应的影响。方法体外利用LPS刺激raw264.7细胞,建立巨噬细胞炎症模型。将巨噬细胞随机分为正常组、模型组、BRPS低、中、高剂量组(BRPS质量浓度分别为25、50、100 mg·L^(-1))。采用细胞增殖检测试剂盒(cell counting kit-8,CCK-8)检测细胞活力;酶联免疫吸附法(enzyme-linked immunosorbent assay, ELISA)检测白介素-1β(interleukin-1β,IL^(-1)β)、白介素-6(interleukin-6,IL-6)以及肿瘤坏死因子-α(tumor necrosis factor alpha, TNF-α)的分泌;采用Western blot法检测环氧合酶-2(cyclooxygenase-2,COX-2)、髓样分化因子88(myeloid differentiation factor 88,MyD88)、白介素-1受体相关激酶1(interleukin-1 receptor-associated kinase1,IRAK1)蛋白的表达以及核转录因子-κB(nuclear factor-кB,NF-кB)、丝裂原活化蛋白激酶(mitogen activated protein kinases, MAPK)、信号传导与转录激活因子-3(signal transducers and activators of transcription-3,STAT3)蛋白的活化水平。结果 LPS在100~2 000μg·L^(-1)内对raw264.7细胞活力无影响;但能够显著上调raw264.7细胞COX-2蛋白的表达,提示巨噬细胞炎症模型构建成功。BRPS在0~100 mg·L^(-1)内对raw264.7细胞活力无影响,不显示细胞毒性;但能够下调LPS诱导的raw264.7细胞IL^(-1)β、IL-6、TNF-α的分泌。与模型组比较,BRPS低、中、高剂量组细胞对IL^(-1)β分泌的抑制率分别为14.2%、47.6%、72.4%,对IL-6分泌的抑制率分别为5.5%、22.2%、45.1%,对TNF-α分泌的抑制率分别为9.5%、29.1%、55.3%。同时,BRPS能够抑制LPS诱导的COX-2蛋白表达,抑制MyD88、IRAK1蛋白表达,抑制NF-кB p65的磷酸化和核内转移,抑制ERK、JNK、p38磷酸化以及STAT3磷酸化。结论 BRPS对LPS诱导的raw264.7巨噬细胞炎症反应具有抑制作用,其作用可能与其抑制NF-кB、MAPK、STAT3活化有关。

关键词：草苁蓉多糖脂多糖 raw264.7 炎症因子