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ratiometric fluorescence probe for accurate detection of Concanavalin A by coupling fluorescent microsphere with boric acid functionalized carbon dots

引用

Chinese Chemical Letters 2024年第2期35卷 460-463页

作者： Mingyue Xie Juan Chen Yufei Wang Bojun Liu Rong-Bin Song Hong-Min Meng Zhaohui Li College of Chemistry Institute of Analytical Chemistry for Life ScienceHenan Joint International Research Laboratory of Green Construction of Functional Molecules and their Bioanalytical ApplicationsZhengzhou UniversityZhengzhou 450001China Zhengzhou Key Laboratory of Forensic Science and Technology Railway Police CollegeZhengzhou 450053China School of Ecology and Environment Zhengzhou UniversityZhengzhou 450001China

Accurate and sensitive strategies for Concanavalin A(Con A)sensing are conducive to the better cognition of various important biological and physiological ***,by designing dextran-functionalized fluorescent microspheres(DxFMs)and boric acid-modified carbon dots(BCDs)as recognition unit and built-in signal reference respectively,a ratiometric fluorescent detection platform was proposed for Con A detection with high *** this protocol,the BCDs/DxFMs precipitation was formed due to the covalent interactions between cis-diol of DxFMs and boronic acid groups of BCDs,thus only fluorescence of BCDs could be detected in the *** Con A was presented,it could bind to DxFMs through its carbohydrate recognition ability and suppress the subsequent assembly between DxFMs and BCDs,leading to the simultaneous capture of DxFMs and BCDs fluorescence in the *** the BCDs content was superfluous,their fluorescence intensities were basically constant in all *** on the unchanged BCDs fluorescence signal and target-dependent DxFMs fluorescence signal in supernatant,the ratiometric detection of Con A was *** optimized conditions,this ratiometric fluorescent platform displayed a linear detection range from 0.125μg/mL to 12.5μg/mL with a detection limit of 0.089μg/***,satisfied analytical outcomes for Con A detection in serum samples were obtained,manifesting huge application potential of this ratiometric fluorescent platform in clinical diagnosis.

关键词： Concanavalin A fluorescent microspheres Carbon dots ratiometric fluorescent assay Reliability

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