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Measurement of Protein 53 Diffusion Coefficient in Live HeLa Cells Using raster Image Correlation Spectroscopy (RICS)

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Journal of Biomaterials and Nanobiotechnology 2010年第1期1卷 31-36页

作者： Sungmin Hong Ying-Nai Wang Hirohito Yamaguchi Harinibytaraya Sreenivasappa Chao-Kai Chou Pei-Hsiang Tsou Mien-Chie Hung Jun Kameoka Department of Electrical and Computer Engineering Texas A & M University College Station Texas USA Department of Molecular and Cellular Oncology University of Texas M. D. Anderson Cancer Center Houston Texas USA Department of Molecular and Cellular Oncology University of Texas M. D. Anderson Cancer Center Houston Texas USA.

We have applied raster Image Correlation Spectroscopy (RICS) technique to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. HeLa cells expressing Green Fluores-cent Protein (GFP) tagged p53 were incubated with and without DNA damaging agents, cisplatin or eptoposide, which are widely used as chemotherapeutic drugs. Then, the diffusion coefficient of GFP-p53 was determined by RICS and it was significantly reduced after the drug treatment while that of the one without drug treatment was not. It is suggested that the drugs induced the interaction of p53 with either other proteins or DNA. Together, our results demonstrated that RICS is able to detect the protein dynamics which may be associated with protein-protein or protein-DNA interactions in living cells and it may be useful for the drug screening.

关键词： raster Image Correlation Spectroscopy Diffusion Coefficient p53 DNA Damage

Flexural strength of fused filament fabricated (FFF) PLA parts on an open-source 3D printer

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Advances in Manufacturing 2018年第4期6卷 430-441页

作者： Shilpesh R. Rajpurohit Harshit K. Dave Department of Mechanical Engineering SV National Institute of Technology Surat Gujarat 395007 India

Fused filament fabrication (FFF) has been widely used to develop prototypes as well as functional parts owing to its capability for creating parts with complex geometries in a short time without the specific requirement of tooling. The mechanical properties of parts produced by FFF exhibit 70%-80% of the mechanical properties of parts produced by injection molding. The mechanical properties of FFF-produced parts are primarily dependent on the selection of various process variables. The mechanical properties of the part can be enhanced through the proper selection of process variables. In the present experimental investigation, the effects of the process variables, viz. raster angle, layer height, and raster width on the flexural properties of FFF-printed polylactic acid (PLA) is studied. The result shows that flexural strength is primarily influenced by layer height followed by raster angle. The sample printed with 100-μm layer height and 0° raster angle exhibits a higher tensile strength. Further, the microscopic examination of the deformed specimen is performed to understand the mode of failure. Specimens printed at different raster angles show different modes of failure.

关键词： Fused filament fabrication (FFF) Polylactic acid (PLA) raster angle Layer height raster width Flexural strength

Spatial variation of the relationship between transport accessibility and the level of economic development in Qinghai-Tibet Plateau, China

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Journal of Mountain Science 2019年第8期16卷 1883-1900页

作者： YANG Xue-ting QIU Xiao-ping FANG Yi-ping XU Yun ZHU Fu-biao Research Center of Sichuan County Economy Development Mianyang Teachers’CollegeMianyang 621000China The faculty Geography Resource Sciences Sichuan Normal UniversityChengdu 610101China Institute of Mountain Hazards and Environment Chinese Academy of SciencesChengdu 610041China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China School of Economics Hefei University of TechnologyHefei 230009China

As an important component of China’ transportation systems, for a long time, the insufficient performance of transport in QinghaiTibet Plateau(QTP) was a bottleneck restricting the economic growth and social development in this area. Nevertheless, the implementation of the western development strategy has accelerated the preliminary construction of comprehensive transport network since 2000. Due to the large area and significant geographical heterogeneity, there is a growing need to understand the relationship between transportation and economic development based on the perspective of spatial difference. By using GIS-based raster analysis and Geographically Weighted Regression(GWR) model, we investigated the spatial-temporal distribution of highway, railway and airport accessibility, respectively, and estimated the correlation and heterogeneity between transport accessibility and the level of economic development. Results revealed that:(1) Transport accessibility in the QTP improved by 53.38% in the past 15 years, which is specifically embodied in the improvement of both highway and railway.(2) Accessibility presented prominent differentiation in the space, increasing from west to east and reducing with the rise of elevation, specifically, the best accessibility area of the highway is below 4000 m above sea level, while the area with an altitude of over 4000 m has the lowest aviation time cost.(3) In general, the long weighted average time cost to critical transport facilities posed significantly negative effect on county economic growth in QTP, more positively, the adverse effect gradually weakened over time.(4) Obvious heterogeneity exists at the influence of different transport accessibility factors on the level of economic development, reflecting both in the horizontal space and altitudinal belt. Therefore, region-specific policies should be addressed for the sustainable development of transport facilities as well as economy in the west mountain areas.

关键词： Accessibility raster cost weighted Geographically weighted regression Economic effect Spatial variations

Optimized cellular automaton for stand delineation

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Journal of Forestry Research 2019年第1期30卷 107-119页

作者： Timo Pukkala University of Eastern Finland

Forest inventories based on remote sensing often interpret stand characteristics for small raster cells instead of traditional stand compartments. This is the case for instance in the Lidar-based and multi-source forest inventories of Finland where the interpretation units are 16 m 9 16 m grid cells. Using these cells as simulation units in forest planning would lead to very large planning problems. This difficulty could be alleviated by aggregating the grid cells into larger homogeneous segments before planning calculations. This study developed a cellular automaton(CA) for aggregating grid cells into larger calculation units, which in this study were called stands. The criteria used in stand delineation were the shape and size of the stands, and homogeneity of stand attributes within the stand. The stand attributes were: main site type(upland or peatland forest), site fertility, mean tree diameter, mean tree height and stand basal area. In the CA, each cell was joined to one of its adjacent stands for several iterations,until the cells formed a compact layout of homogeneous stands. The CA had several parameters. Due to high number possible parameter combinations, particle swarm optimization was used to find the optimal set of parameter values. Parameter optimization aimed at minimizing within-stand variation and maximizing between-stand variation in stand attributes. When the CA was optimized without any restrictions for its parameters, the resulting stand delineation consisted of small and irregular stands. A clean layout of larger and compact stands was obtained when the CA parameters were optimized with constrained parameter values and so that the layout was penalized as a function of the number of small stands(<0.1 ha). However, there was within-stand variation in fertility class due to small-scale variation in the data. The stands delineated by the CA explained 66–87% of variation in stand basal area, mean tree height and mean diameter, and 41–92% of variation in the fertility class of the site. It was con

关键词： Forest planning Particle swarm optimization raster data Segmentation Spatial optimization

raster flite视景系统数据库建模分析

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中国民航飞行学院学报 2009年第6期20卷 16-19页

作者：陈又军苏彬米祖强中国民航飞行学院四川广汉618307

视景系统为飞行员提供虚拟的外部世界,它是模拟飞行仿真的关键技术之一。视景系统是否真实取决于机场视景数据库的建模逼真度。本文分析了raster flite视景系统数据库的特点、开发过程和在全任务飞行模拟机上的安装、调试方法。其主要... 详细信息

视景系统为飞行员提供虚拟的外部世界,它是模拟飞行仿真的关键技术之一。视景系统是否真实取决于机场视景数据库的建模逼真度。本文分析了raster flite视景系统数据库的特点、开发过程和在全任务飞行模拟机上的安装、调试方法。其主要开发工具采用Multigen Creator、Photoshop和raster flite视景数据库开发软件包。

关键词： raster flite 视景系统机场视景数据库模拟机飞行仿真

AN INTEGRATED DATA MODEL IN THREE DIMENSIONAL GIS

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Geo-Spatial Information Science 1999年第1期2卷 1-8,15页

作者： Gong Jianya Xia Zongguo National Laboratory for Information Engineering in Surveying Mapping and Remote Sensing WTUSM Wuhan China

The current GIS can only deal with 2-D or 2.5-D information on the earth surface. A new 3-D data structure and data model need to be designed for the 3-D GIS. This paper analyzes diverse 3-D spatial phenomena from mine to geology and their complicated relations, and proposes several new kinds of spatial objects including cross-section, column body and digital surface model to represent some special spatial phenomena like tunnels and irregular surfaces of an ore body. An integrated data structure including vector, raster and object-oriented data models is used to represent various 3-D spatial objects and their relations. The integrated data structure and object-oriented data model can be used as bases to design and realize a 3-D geographic information system.

关键词： 3-D geographic information system object-oriented data model vector raster

4D DATA FUSION TECHNIQUE IN URBAN WATERLOG-DRAINING DECISION SUPPORT SYSTEM

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Geo-Spatial Information Science 2000年第3期3卷 42-46页

作者： Li Jun Bian Fuling School of Information Engineering WTUSM Wuhan China

This paper studies urban waterlog_draining decision support system based on the 4D data fusion technique.4D data includes DEM,DOQ,DLG and DRG.It supplies entire databases for waterlog forecast and analysis together with non_spatial fundamental database.Data composition and reasoning are two key steps of 4D data fusion.Finally,this paper gives a real case: Ezhou Waterlog_Draining Decision Support System (EWDSS) with two application models,i.e.,DEM application model,water generating and draining model.

关键词： 4D data fusion raster vector

HUBEX强化观测期雷达测雨在水文过程模拟中的应用(英)

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Advances in Atmospheric Sciences 2003年第2期20卷 205-212页

作者：任立良李春红王美荣 Department of Hydrology and Water Resources Hohai University

On the basis of Digital Elevation Model data, the raster flow vectors, watershed delineation, and spatial topological relationship are generated by the Martz and Garbrecht method for the upper area of Huangnizhuang station in the Shihe Catchment with 805 km2 of area, an intensified observation field for the HUBEX/GAME Project. Then, the Xin’anjiang Model is applied for runoff production in each grid element where rain data measured by radar at Fuyang station is utilized as the input of the hydrological model. The elements are connected by flow vectors to the outlet of the drainage catchment where runoff is routed by the Muskingum method from each grid element to the outlet according to the length between each grid and the outlet. The Nash-Sutcliffe model efficiency coefficient is 92.41% from 31 May to 3 August 1998, and 85.64%, 86.62%, 92.57%, and 83.91%, respectively for the 1st, 2nd, 3rd, and 4th flood events during the whole computational period. As compared with the case where rain-gauge data are used in simulating the hourly hydrograph at Huangnizhuang station in the Shihe Catchment, the index of model efficiency improvement is positive, ranging from 27.56% to 69.39%. This justifies the claim that radar-measured data are superior to rain-gauge data as inputs to hydrological modeling. As a result, the grid-based hydrological model provides a good platform for runoff computation when radar-measured rain data with highly spatiotemporal resolution are taken as the input of the hydrological model.

关键词： digital elevation model raster catchment radar hydrological processes modeling

The Multiresolution Image Format

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Journal of Geographic Information System 2013年第1期5卷 87-95页

作者： Titusz Bugya ákos Halmai University of Pécs Doctoral School of Earth Sciences Pécs Hungary University of Pécs Institute of Geography Pécs Hungary

In this paper we present a new method and file format which allows to create, store and manipulate multiresolution raster images. “Classic” raster images are built up by rasters (pixels) where in the same image each raster (pixel) has the same size. Multiresolution raster image (mri) are unlike the “classic” raster images. An mri file is a regular text file, conformed xml 1.0 standard and allows: 1) to store raster based images with different resolution in the same file and in the same time (i.e., allows to contain rasters with different size in the same image);2) to export easily any part of the image as a separate raster image and to import an image as a part of a raster;3) to define geographical reference for a raster image and use this image as a raster based GIS model;4) to perform computations (i.e., image manipulation) on the content of the file. It is an important characteristic of the mri format that mathematical operations could be performed always in the same resolution as the current resolution of the given part of the image. To confirm this statement we present how to perform a convolution on an mri file.

关键词： Multiresolution Image Storing raster GIS