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Whole-genome rnai screen identifies methylation-related genes influencing lipid metabolism in Caenorhabditis elegans

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Journal of Genetics and Genomics 2018年第5期45卷 259-272页

作者： Xiaotong Zhu Yangli Liu Hong Zhang Pingsheng Liu National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Lipid droplets （LDs） are highly conserved multifunctional cellular organelles and aberrant lipid storage in LDs can lead to many metabolic diseases. However, the molecular mechanisms governing lipid dynamic changes remain elusive, and the high-throughput screen of genes influencing LD morphology was limited by lacking specific LD marker proteins in the powerful genetic tool Caenorhabditis elegons. In this study, we established a new method to conduct whole-genome rnai screen using LD resident protein DHS-3 as a LD marker, and identified 78 genes involved in significant LD morphologic changes. Among them, mrhf-1, as well as a series of methylation-related genes, was found dramatically influencing lipid metabolism. SREBP-1 and SCD1 homologs in C. elegans were involved in the lipid metabolic change of rnthf-1（rnai） worms, and the regulation of ATGL-1 also contributed to it by decreasing triacylglycerol （TAG） hydrolysis. Overall, this study not only identified important genes involved in LD dynamics, but also provided a new tool for LD study using C. elegans, with implications for the study of lipid metabolic diseases.

关键词： Lipid droplet C.elegans rnai screen Lipid metabolism One-carbon metabolism

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A whole-genome rnai screen identifies the genes required for synaptic vesicle and dense-core vesicles cycle

A whole-genome RNAi screen identifies the genes required for...

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The First International Symposium on Membrane Biology--The Tenth National Symposium on Membrane Biology

＜正＞Synaptic vesicles（SV）and dense-core vesicles（DCV）play an important role in the exocytosis of neurotransmitter,hormone and *** exact mechanism of exocytosis and endocytosis for these vesicles,however,remains *** order to get a clear picture of these processes,we aimed to discover novel genes required for the recycle of SV and DCV via

关键词： rnai screen synaptic vesicle dense-core vesicles membrane recycle

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Focused piRNA pathway screens identified multiple pathways involved in heterochromatin formation

Focused piRNA pathway screens identified multiple pathways i...

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中国生物化学与分子生物学会2019年全国学术会议暨学会...

作者： Na Miao Ming Yang Peng Zhou Xin Lu Yang Yu Institute of Biophysics Key Laboratory of RNA Biology Chinese Academy of Sciences University of Chinese Academy of Sciences

Piwi-interacting RNA(piRNA) pathway is a small RNA based "innate immune"" system to defend transposons in animal germlines. About 200～300 genes have been identified as piRNA pathway candidate genes in Drosophila. Panoramix(Panx) links Piwi:piRNA complexes and general heterochromatin machinery. However, the mechanism of Panx-mediated heterochromatin formation is unclear. Here, we report a small scale rnai screen to identify factors required for Panx induced heterochromatin formation. We identified ～40 genes that act downstream of Panx. dNxf2 is one of top candidates in the screen. It was found that dNxf2 functions to silence piRNA guided transposon as a complex with Panx and dNxt1. CG14438 is another top candidates whose function in heterochromatin formation is unknown, although it has been copurified with HP1 a. Loss of CG14438 leads to animal sterility and transposon overexpression, similar to other core piRNA pathway components. Further detailed dissection about the mechanism of CG14438 mediated heterochromatin formation will be presented.

关键词： piRNA rnai screen Heterochromatin

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