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Differential RNA Editing of Mitochondrial Genes in WA-Cytoplasmic Based Male Sterile Line Pusa 6A, and Its Maintainer and Restorer Lines

引用

Rice science 2019年第5期26卷 282-289页

作者： Umakanta NGANGKHAM Swarup Kumar PARIDA Ashok Kumar SINGH Trilochan MOHAPATRA National Research Centre on Plant Biotechnology New Delhi 110012India Indian Council of Agricultural Research-Research Complex for North East Hill Region Indian Council of Agricultural ResearchUmiam 793103India National Institute of Plant Genome Research New Delhi 110067India Division of Genetics Indian Agricultural Research InstituteNew Delhi 110012India Indian Council of Agricultural Research New Delhi 110001India

RNA editing changes the nucleotides at the transcript level of mitochondrial genes which results in synthesis of functional *** study was designed to find the editing sites which could be implicated in male fertility restoration and to develop editing based markers for differentiation of cytoplasmic male sterility and maintainer lines from each *** and RNA from young panicles were isolated from three-line system of hybrid rice PRH10,wild abortive(WA)cytoplasm based male sterile(A line Pusa 6A),maintainer(B line Pusa 6B)and restorer(R line PRR78)*** 6A and PRR78 having the same WA cytoplasm are allo-nuclear and iso-cytpolasmic *** genomic and cDNA amplicons for eight mitochondrial genes(18SrRNA,atp6,atp9,cobII,coxI,coxIII,nadI and rps3)were sequenced and *** in genomic and cDNA sequences were considered as *** hundred and thirty editing sites having base substitution or insertion/deletion were identified with the highest in 18SrRNA(5.74%)and the lowest in coxI(0.60%).The highest editing sites were observed in fertile maintainer Pusa 6B followed by PRR78 and Pusa 6A,of which random five editing sites in five different rice mitochondrial transcripts namely atp9,cobII,coxIII,rps3 and 18SrRNA were chosen and validated through cleaved amplified polymorphism sequence(CAPS)analysis and found to be partially edited in four *** identical editing sites of different mitochondrial genes from maintainer and restorer lines might reflect their possible contribution to fertility restoration of sterile WA cytoplasm.

关键词： cleaved amplified polymorphism sequence cytoplasmic male sterility hybrid rice rnaediting mitochondrial gene wild abortive nucleotide

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