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Target Recycling transcription of Lighting-Up rna Aptamers for Highly Sensitive and Label-Free Detection of ATP

Journal of Analysis and Testing 2021年第2期5卷 174-180页

作者： Jia Lun He Bing Ying Jiang Wen Jiao Zhou Ruo Yuan Yun Xiang School of Chemistry and Chemical Engineering Chongqing University of TechnologyChongqing 400054China Key Laboratory of Luminescence Analysis and Molecular Sensing Ministry of EducationSchool of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China

We describe here a target recycling transcription of lighting-up aptamer strategy for detecting ATP in human serums in a label-free means with high *** molecules specifically recognize the binding aptamer and result in the structure switching of the DNA assembly probes to imitate the target ATP molecule recycling cycles through the toehold-mediated strand displacement reaction,which causes the formation of many dsDNAs containing the rna promoter sequences for subsequent transcription generation of large amounts of lighting-up *** organic dye,malachite green,then associates with these lighting-up aptamers to produce significantly enhanced fluorescence signals,which can sensitively detect ATP within a dynamic range from 10 to 500 nM in a label-free *** sensing approach shows a detection limit of 7.3 nM and also has an excellent selectivity for ATP analogue *** addition,this method can detect ATP molecules in diluted human serum samples sensitively,which proves the promising potential to diagnose ATP-related diseases.

关键词： ATP Toehold strand displacement reaction Lighting-up aptamer rna transcription Recycling amplification

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How to make the end of a gene,the simple way

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BIOCELL 2022年第6期46卷 1453-1457页

作者： KAREL H.M.VAN WELY Centro Nacional de Biotecnología/CSIC Madrid28049Spain

transcription termination of nearly all protein-coding genes in mammals requires 3’end processing by a multiprotein complex that will cleave and polyadenylate the messenger rna *** a variety of enzyme complexes intervene,3’end processing was thought to be fundamentally complex and subject to a multitude of regulatory *** possibility to select just one out of several polyadenylation sites,in particular,has caused much questioning and *** appear to be separate mechanisms however can be combined into a defined set of rules,allowing for a relatively simple interpretation of 3’end ***,readiness of the terminal exon splice site determines when a transcript reaches the maturity to select a nearby polyadenylation *** pausing then acts in concert,extending the timeframe during which the transcription complex is close to polyadenylation *** rna polymerase pausing is governed by the same type of sequences in bacteria and metazoans,mammalian transcription termination resembles its prokaryote counterpart more than generally thought.

关键词： rna transcription rna polymerase II pausing Splicing Termination Polyadenylation

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柑橘黄龙病菌(Candidatus Liberibacter asiaticus)分泌蛋白基因DNA含量及其rna表达量关系分析（英文）

柑橘黄龙病菌(Candidatus Liberibacter asiaticus)分泌蛋白基因D...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者：李宾宾 Yang Yi Liu Zhixin Wang Jianhua Yu Naitong Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences 海南大学 Environment and Plant Protection Institute Chinese Academy of Tropical Agricultural Sciences

Huanglongbing(HLB) is the most destructive disease of citrus *** disease is caused by Candidatus Iiberibacter spp.,which vectored by the psyllids Diaphorina citri Kuwayama and Trioza *** proteins are important in bacterial pathogenesis and structure *** of them express at high *** obtain at least one high-level expression of secretory protein genes(SPGs),ten candidated SPGs were chosen from the Candidatus Iiberibacter asiaticus by bioinformatic *** SPGs were tested by PCR,qPCR and RT-qPCR method,*** result showed that two SPGs,408 and pap,are highly *** 408 and pap genes were further constructed into the plant expression vector pCAMBIA 1300(GV1300:GFP) and expressed in tobacco leaf epidermal cells for subcellular localization *** transient expression results indicated that 408 protein is located in the nucleus and cytoplasm of tobacco leaf ***,pap protein was located in the radially arranged micro-fibrils of tobacco guard cells that may relate to help pathogen invade into plant *** this study,408 and pap secretory protein genes of Candidatus Iiberibacter asiaticus were obtained and the subcellular localization of these two genes in tobacco leaves was *** research laies an important foundation for the preparation of the antiserum against the Candidatus Iiberibacter asiaticus and the early detection and prevention of citrus HLB.

关键词： Candidatus Liberibacter asiaticus Secretory protein DNA amount rna transcription Subcellular localization

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