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Engineering Multipedal DNA Walker on Paper for Sensitive Electrochemical Detection of Plant Microrna

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Chinese Journal of Chemistry 2022年第23期40卷 2808-2814页

作者： Xiaojuan Liu Junzhu Xiang Hao Cheng Yuying Wang Feng Li College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural UniversityQingdaoShandong 266109China College of Plant Health&Medicine Qingdao Agricultural UniversityQingdaoShandong 266109China

Micrornas (mirnas) are vital regulators in both plants and animals. Therefore, it is highly desirable to develop portable and user-friendly biosensors for convenient and sensitive detection of mirnas. Herein, a novel paper-based electrochemical biosensor was intelligently engineered for the detection of plant mirna based on a smart DNA walking machine and λ-exonuclease (λ-Exo)-assisted target recycling amplification. Using TaMIR5086 as a target plant mirna, the presence of TaMIR5086 could initiate the target recycling process and activate the DNA walker to move along the track on the paper. Then, numerous electroactive molecules-labeled single-strand DNA (ssDNA) would be released and adsorbed onto the surface of screen-printed electrode, generating a remarkably increased electrochemical signal. Benefitting from the dual amplification, the developed biosensor exhibits excellent analytical performance toward TaMIR5086 with a detection limit down to 0.37 pmol/L. Furthermore, the paper-based biosensor could be applied for the analysis of target mirna in complex biological samples, which found great potential in the fields of mirna analysis and plant biology research.

关键词： rna recognition Electrochemistry Sensor DNA walker on paper Target recycling amplification

rna Imaging in Live Cells

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Chinese Journal of Chemistry 2023年第19期41卷 2531-2535页

作者： Jingfang Zhao Limin Xiang College of Chemistry and Molecular Sciences&TaiKang Center for Life and Medical Sciences Wuhan UniversityWuhanHubei430072 China

Traditional molecular biology tools have elucidated the identities and functions of rna molecules,which are essential to the understanding of gene transcription and protein translation.Deepening this research field would further require the direct visualization of rna dynamics such as the DNA-rna interactions and rna-protein interactions.Towards this goal,the rise of rna imaging tools over the past 15 years has reformed how we looked at these processes.In this Emerging Topic,we first highlighted recent advances on three main rna imaging tools based on the species of interacting molecules:rna-rna pairing,rna-protein binding,and small molecule-rna complex.We introduced the advantages of these tools from a technical viewpoint,including binding affinity,fluorescent turn-on ratio,stability,and impacts on targeted rna.Next,we discussed new rising opportunities and future directions,echoing the state-of-the-art imaging tools in the fields of fluorescent proteins and small fluorescent molecules.Together,we believe this emerging field will bring new insights on how we study rna biology in living systems.

关键词： Fluorescent imaging rna imaging Fluorogenic rna rna Fluorescent probes rna recognition

How Complex Are the Editosomes in Plant Organelles?

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Molecular Plant 2014年第4期7卷 582-585页

作者： Mizuki Takenaka Molekulare Botanik Universitat Ulm 89069 Ulm Germany

In chloroplasts and mitochondria of most land plants, rna editing modifies specific nucleotides. The individual nucleotides are identified in the complex rna molecule population of either organelle by their sequence context. The biochemical reaction is in effect a C-to-U deamination in flowering plants, in addition a U-to-C amination reaction occurs in ferns and hornworts.

关键词： STRUCTURAL BASIS rna recognition PPR PROTEINS ARABIDOPSIS MITOCHONDRIA PLASTIDS

Developing rna Duplex-Binding Peptide Nucleic Acids for Biotechnological Applications

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Developing RNA Duplex-Binding Peptide Nucleic Acids for Biot...