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Translation landscape of SARS-CoV-2 noncanonical subgenomic rnas

Virologica Sinica 2022年第6期37卷 813-822页

作者： Kai Wu Dehe Wang Junhao Wang Yu Zhou State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan430072China TaiKang Center for Life and Medical Sciences RNA InstituteWuhan UniversityWuhan430072China Institute for Advanced Studies Wuhan UniversityWuhan430072China Frontier Science Center for Immunology and Metabolism Wuhan UniversityWuhan430072China

The ongoing COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)with a positive-stranded rna genome. Current proteomic studies of SARS-CoV-2 mainly focus on the proteins encoded by its genomic rna(grna) or canonical subgenomic rnas(sgrnas). Here, we systematically investigated the translation landscape of SARS-CoV-2, especially its noncanonical sgrnas. We first constructed a strict pipeline, named vipep, for identifying reliable peptides derived from rna viruses using rna-seq and mass spectrometry data. We applied vipep to analyze 24 sets of mass spectrometry data related to SARS-CoV-2infection. In addition to known canonical proteins, we identified many noncanonical sgrna-derived peptides,which stably increase after viral infection. Furthermore, we explored the potential functions of those proteins encoded by noncanonical sgrnas and found that they can bind to viral rnas and may have immunogenic activity. The generalized vipep pipeline is applicable to any rna viruses and these results have expanded the SARSCoV-2 translation map, providing new insights for understanding the functions of SARS-CoV-2 sgrnas.

关键词： SARS-CoV-2 Subgenomic rna(sgrna) Mass spectrometry Translation rna binding

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Design and Synthesis of Paromomycin Related Heterocyclic Aminogly coside Mimetics Based on the Mass Spectroscopy rna binding Assay

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有机化学 2003年第Z1期23卷 4-4页

作者： DING Yi-Li Ribapharm Inc. 3300 Hyland Avenue Costa Mesa CA 92626 USA

　　Aminoglycoside antibiotics are known to interact with a variety of rna molecules, including ribosomal rna, and the hammerhead ribozyme. These low molecular weight antibiotics have also been found to block the binding of HIV-1 Rev protein to its viral rna recognition site, thereby inhibiting the production of the virus. But due to their toxicity, poor bioavailability, cellular penetration, and instability, they can not be used as an inhibitory drug direct ly. Therefore, it is highly desirable to synthesize aminoglycoside mimetics, which may be less toxic and more active than original aminoglycosides.……

关键词： rna binding low molecular weight hammerhead ribozyme

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