检索结果-南通市图书馆

miR-16-5p enhances sensitivity to rg7388 through targeting PPM1D expression(WIP1)in Childhood Acute Lymphoblastic Leukemia

引用

Cancer Drug Resistance 2023年第2期6卷 242-256页

作者： Maryam Zanjirband Soheila Rahgozar Narges Aberuyi Department of Cell and Molecular Biology&Microbiology Faculty of Biological Science and TechnologyUniversity of IsfahanIsfahan 15100Iran.

Aim:Given the encouraging results of the p53-Mdm2 inhibitor rg7388 in clinical trials and the vital function of miR-16-5p in suppressing cell proliferation,the aim of the present study was to investigate the combined impact of rg7388 and miR-16-5p overexpression on the childhood acute lymphoblastic leukemia(chALL).Methods:miRTarBase and miRDB,along with KEGG and STRING databases,were used to predict miR-16-5p target genes and explore protein-protein interaction networks,respectively.B-and T-lymphoblastic cell lines,in addition to patient primary cells,were treated with *** overexpression of miR-16-5p in Nalm6 cell line was induced through cell electroporation and transfection of microRNA mimics was confirmed by *** viability was evaluated using the MTT *** blot analyses were performed to evaluate the effects of rg7388 and miR-16-5p upregulation on the protein levels of p53 and its downstream target genes in chALL *** sample t-test was employed for statistical ***:MTT assay showed rg7388-induced cytotoxicity in wild-type p53 Nalm6 cell line and p53 functional patient primary ***,CCRF-CEM and p53 non-functional leukemic cells indicated drug *** blot analyses validated the bioinformatics results,confirming the downregulation of WIP1,p53 stabilization,as well as overexpression of p21WAF1 and Mdm2 proteins in Nalm6 cells transfected with ***,enhanced sensitivity to rg7388 was observed in the transfected ***:This is the first study indicating the mechanistic importance of miR-16-5p overexpression in chALL and its inhibitory role in leukemia treatment when combined with the p53-Mdm2 antagonist,*** findings might be useful for researchers and clinicians to pave the way for better management of chALL.

关键词： Pediatric ALL miR-16-5p rg7388 PPM1D p53

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：