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Micro rNA-320 family is downregulated in colorectal adenoma and affects tumor proliferation by targeting CDK6

World Journal of Gastrointestinal Oncology 2016年第7期8卷 532-542页

作者： Toshihiro Tadano Yoichi Kakuta Shin Hamada Yosuke Shimodaira Masatake Kuroha Yoko Kawakami Tomoya Kimura Hisashi Shiga Katsuya Endo Atsushi Masamune Seiichi Takahashi Yoshitaka Kinouchi Tooru Shimosegawa Division of Gastroenterology Tohoku University Graduate School of Medicine Community Gastroenterology (Iwaki Kyoritsu Hospital) Tohoku University Graduate School of Medicine Health Administration Center Center for the Advancement of Higher Education Tohoku University

AIM: To investigate the micror NA(mir NA) expression during histological progression from colorectal normal mucosa through adenoma to carcinoma within a lesion. METHODS: Using microarray, the sequential changes in mir NA expression profiles were compared in colonic lesions from matched samples; histologically, nonneoplastic mucosa, adenoma, and submucosal invasive carcinoma were microdissected from a tissue sample. Cell proliferation assay was performed to observe the effect of mir NA, and its target genes were predicted using bioinformatics approaches and the expression profile of SW480 transfected with the mir NA mimics. mr NA and protein levels of the target gene in colon cancer cell lines with a mimic control or mir NA mimics were measured using qr TPCr and Western blotting. The expression levels of mir NA and target gene in colorectal tissue samples were also ***: Microarray analysis identified that the mir-320 family, including mir-320 a, mir-320 b, mir-320 c, mir-320 d and mir-320 e, were differentially expressed in adenomaand submucosal invasive carcinoma. The mi r-320 family, which inhibits cell proliferation, is frequently downregulated in colorectal adenoma and submucosal invasive carcinoma tissues. Seven genes including CDK6 were identified to be common in the results of gene expression array and bioinformatics analyses performed to find the target gene of the mir-320 family. We confirmed that mr NA and protein levels of CDK6 were significantly suppressed in colon cancer cell lines with mir-320 family mimics. CDK6 expression was found to increase from non-neoplastic mucosa through adenoma to submucosal invasive carcinoma tissues and showed an inverse correlation with mir-320 family ***: Mir-320 family affects colorectal tumor proliferation by targeting CDK6, plays important role in its growth, and is considered to be a biomarker for its early detection.

关键词： CDK6 Colorectal cancer Mi r-320 family Colorectal adenoma Laterally spreading type

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微小rNA-320在肿瘤中的研究进展

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肿瘤防治研究 2015年第3期42卷 311-314页

作者：万璐颖邓军张凌项晓军陈俊熊建萍南昌大学第一附属医院肿瘤内科南昌330006

微小rNA(mi rNAs)是一类细胞内源性表达的小分子非编码rNA,具有转录后调控基因表达的作用。近年来,在结肠癌、乳腺癌和胆管癌等多种肿瘤中发现mi r-320表达下调,提示mi r-320可通过抑制细胞增殖和侵袭等方式抑制肿瘤进展。对于mi r-320... 详细信息

微小rNA(mi rNAs)是一类细胞内源性表达的小分子非编码rNA,具有转录后调控基因表达的作用。近年来,在结肠癌、乳腺癌和胆管癌等多种肿瘤中发现mi r-320表达下调,提示mi r-320可通过抑制细胞增殖和侵袭等方式抑制肿瘤进展。对于mi r-320调控的靶基因及相关信号通路的深入研究,有助于阐明肿瘤发生发展的分子机制,并有望为临床肿瘤治疗提供新的靶点。本文就mi r-320在肿瘤中的研究进展作一综述。

关键词： mi rNA mi r-320 肿瘤靶基因

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