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Screening strategies for quorum sensing inhibitors in combating bacterial infections

Journal of Pharmaceutical Analysis 2022年第1期12卷 1-14页

作者： Lan Lu Mingxing Li Guojuan Yi Li Liao Qiang Cheng Jie Zhu Bin Zhang Yingying Wang Yong Chen Ming Zeng Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province Sichuan Industrial Institute of AntibioticsSchool of PharmacyChengdu UniversityChengdu610000China Laboratory of Molecular Pharmacology Department of PharmacologySchool of PharmacySouthwest Medical UniversityLuzhou646000SichuanChina

Interference with quorum sensing(QS)represents an antivirulence strategy with a significant promise for the treatment of bacterial infections and a new approach to restoring antibiotic *** the past two decades,a novel series of studies have reported that quorum quenching approaches and the discovery of quorum sensing inhibitors(QSIs)have a strong impact on the discovery of anti-infective drugs against various types of *** discovery of QSI was demonstrated to be an appropriate strategy to expand the anti-infective therapeutic approaches to complement classical antibiotics and antimicrobial *** the discovery of QSIs,diverse approaches exist and develop in-step with the scale of screening as well as specific QS *** review highlights the latest findings in strategies and methodologies for QSI screening,involving activity-based screening with bioassays,chemical methods to seek bacterial QS pathways for QSI discovery,virtual screening for QSI screening,and other potential tools for interpreting QS signaling,which are innovative routes for future efforts to discover additional QSIs to combat bacterial infections.

关键词： quorum sensing inhibitor quorum quenching Anti-infective agent Screening strategies

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Bioaugmentation and quorum sensing disruption as solutions to increase nitrate removal in sequencing batch reactors treating nitrate-rich wastewater

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Journal of Environmental Sciences 2020年第12期32卷 179-185页

作者： Xinyi Ruan Jun Yin Xueyan Cui Na Li Dongsheng Shen School of Environmental Science and Engineering Zhejiang Gongshang UniversityHangzhou 310012PR China Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling Hangzhou 310012PR China

Bioaugmentation of denitrifying bacteria can serve as a promising technique to improve nutrient removal during wastewater treatment. While denitrification inhibition by bacterial quorum sensing(QS) in Pseudomonas aeruginosa has been indicated, the application of bacterial QS disruption to improve nitrate removal from wastewater has not been investigated. In this study, the effect of bioaugmentation of P. aeruginosa SD-1 on nitrate removal in sequencing batch reactors that treat nitrate rich wastewater was assessed. Additionally, the potential of a quorum sensing inhibitor(QSI) to improve denitrification following bacterial bioaugmentation was evaluated. Curcumin, a natural plant extract, was used as a QSI. The chemical oxygen demand(COD) and initial nitrate concentration of the influent were 700 ±20 mg/L and 200 ±10 mg/L respectively, and their respective concentrations in the effluent were 56.9 ±3.2 mg/L and 9.0 ±3.2 mg/L. Thus, the results revealed that bioaugmentation of P. aeruginosa SD-1 resulted in an increased nitrate removal to 82% ±1%. Further, nitrate was almost completely removed following the addition of the QSI, and activities of nitrate reductase and nitrite reductase increased by 88% ±2% and 74% ±2% respectively. The nitrogen mass balance indicated that aerobic denitrification was employed as the main pathway for nitrogen removal in the reactors. The results imply that bioaugmentation and modulation of QS in denitrifying bacteria, through the use of a QSI, can enhance nitrate removal during wastewater treatment.

关键词： Bioaugmentation Denitrification Nitrate removal quorum sensing inhibitor Curcumin

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Degradation of N-Acyl Homoserine Lactone quorum sensing Signals by Bacillus thuringiensis AHL Lactonase

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Advances in Microbiology 2023年第11期13卷 526-538页

作者： Waeel H. Alramadhan Anthony Ejiofor Terrance Johnson Department of Biological Sciences Tennessee State University Nashville Tennessee USA

Bacterial cells rely on signaling molecules to communicate with others from the same species and induce certain genes in a process known as quorum sensing (QS). A common molecule is N-acyl homoserine lactone (AHL) which is responsible for the expression of virulence and other factors that allow the organisms to compete in a given environment. On the other hand, other bacteria produce certain enzymes such as AHL-lactonase that break down AHL molecules and prevent gene expression of these factors. The aim of this work was to examine the level of degradation of AHL molecules by AHL-lactonase in 62 Bacillus thuringiensis (Bt) strains isolated from Middle Tennessee, Mississippi, and Alabama. N-hexanoyl-homoserine lactone (C6-HSL) and N-3-oxo-hexanoyl homoserine lactone (3-oxo-C6-HSL), which cause Chromobacterium violaceum (CV026) to produce a purple pigment were tested at different concentrations to view the Bt lactonase activity. In addition, PCR was used to test for the presence of the lactonase gene. The results showed that among the 62 Bt strains, there were 58 that possessed the AHL-lactonase (aiiA) gene and 48 strains were able to degrade C6-HSL. At high concentrations of AHL, only 13 strains were able to completely degrade C6-HSL. In addition, degradation of 3-oxo-C6-HSL was weak compared to C6-HSL. The results also revealed that AHL lactonase was thermostable, and it was concluded that the level of degradation varies in Bt strains. Only 13 of the strains studied have potent inhibitory activity against C6-HSL, which may be good to be used in field applications to control agricultural pest.

关键词： quorum sensing quorum sensing inhibitor N-Acyl Homoserine Lactone AHL Lactonase Bacillus thuringiensis CV026

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Catechol-derived inhibitors against quorum sensing from Retiboletus kauffmanii

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Medicinal Plant 2021年第1期12卷 68-70页

作者： Liman ZHOU Lin WANG Fandong KONG Qingyun MA Qingyi XIE Haofu DAI Youxing ZHAO Haikou Key Laboratory for Research and Utilization of Tropical Natural Products Institute of Tropical Bioscience and BiotechnologyCATASHaikou 571101China Hainan Academy of Tropical Agricultural Resource CATASHaikou 571101China

[Objectives]This study aimed to screen anti-quorum sensing(QS)activity from the constituent of Retiboletus kauffmanii fruit body.[Methods]Chromatographic technology was used to isolate compounds,while well diffusion assay was applied to screen anti-quorum sensing activity.[Results]12 phenolic compounds were purified from extracts,three catechol-derived compounds at sub-inhibitory concentrations were found to inhibit the production of violacein in Chromobacterium violaceum for the first time.[Conclusions]Three catechol analogs isolated from *** fruit body extract were indicated as quorum sensing inhibitors against ***.

关键词： Chromobacterium violaceum quorum sensing inhibitor Retiboletus kauffmanii Virulence factors

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Chlorogenic acid attenuates virulence factors and pathogenicity of Pseudomonas aeruginosa by regulating quorum sensing

Chlorogenic acid attenuates virulence factors and pathogenic...

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华东六省一市生物化学与分子生物学会联盟2019年学术与...

作者： Hong Wang Weihua Chu Chao Ye Bruno Gaeta Huimin Tao Min Wang Zheng Qiu

quorum sensing(QS) is a cell-to-cell communication that is used by bacteria to regulate collective *** sensing controls virulence factor production in many bacterial species and it is regarded as an attractive target to combat bacterial pathogenicity,especially against antibiotic-resistant *** acid(CA),abundant in fruits,vegetables,and Chinese herbs,processes multiple *** this research,we explored its quorum sensing quenching *** Pseudomonas aeruginosa,CA significantly inhibited the formation of biofilm,the ability of swarming,and virulence factors including protease and elastase activities and rhamnolipid and pyocyanin *** showed similar inhibitory effects in Chromobacterium violaceum on its biofilm formation,swarming motility,chitinolytic activity and violacein *** examined the expression of QS-related genes in *** and found these genes were all down regulated by CA *** modeling revealed that CA can form hydrogen bonds with all three QS *** elegans and mouse infection models were employed to explore the anti-virulence ability of CA and its effect on pathogenesi sprocess in *** extended the survival period and reduced the quantity of *** in nematode gut,showing a moderate protective effect on *** mice wound model,CA-treated groups showed an accelerating healing rate and the bacteria number in wound area was also decreased by CA *** is suggested by our research that CA has potential to be used as an anti-virulence factor in *** infection.

关键词： chlorogenic acid quorum sensing inhibitor Pseudomonas aeruginosa anti-virulence

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