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Identification of quinolones/fluoroquinolones Resistance Genes from Staphylococci Strains Isolated at the University Hospital of Brazzaville, Republic of the Congo

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Journal of Biosciences and Medicines 2023年第2期11卷 30-52页

作者： Léa Gwladys Gangoue Faust René Okamba Ondzia Stech Anomene Eckzechel Nzaou Fils Landry Mpele Tarcisse Baloki Ngoulou Fabien Rock Niama Rachel Moyen Etienne Nguimbi Molecular and Cellular Laboratory Faculty of Sciences and Techniques Marien Ngouabi University Brazzaville Republic of the Congo Unit of Molecular Microbiology and Bioinformatics Faculty of Science and Techniques Marien Ngouabi University Brazzaville Republic of the Congo National Public Health Laboratory Brazzaville Republic of the Congo Faculty of Health Sciences Marien Ngouabi University Brazzaville Republic of the Congo National Institute for Research in Natural Sciences (IRSEN) Brazzaville Republic of the Congo

Staphylococci strains, like the majority of bacterial strains, have developed the resistance to several antibiotics, including quinolones and Fluoroquin-olones In the Republic of the Congo, cases of resistance leading to treat-ment failures have been observed during the treatment of staphylococcal infections with antibiotics in hospitals. The objective of this study was to identify the Quinolone/Fluoroquinolone resistance genes from staphylo-cocci strains isolated in hospitals. A total of 51 strains of Staphylococci were isolated, including 16 (31.37%) community strains, and 35 (68.62%) clinical strains. 46 strains of Staphylococcus aureus (S. aureus) and 5 SCNs were identified. A total of 34 DNA fragments from different strains resistant to quinolones/fluoroquinolones, including 21 (61.67%) DNA fragments from clinical S. aureus and 13 (38.23%) from community SCN strains were analyzed by the molecular method (genotypic detection) by PCR. The genotypic results made it possible to identify the gyrA, grLA and norA genes and to show that these genes are involved in the resistance of the strains to the various antibiotics used. The grLA gene was the most identified gene with a frequency of 75%. The gyrA and grLA genes have been identified in Staphylococcus aureus and Coagulase Negative Staphy-lococci. The norA gene, on the other hand, has only been identified in Staphylococcus aureus. Two mechanisms are essentially involved in the resistance of Staphylococci to quinolones/fluoroquinolones, the mecha-nism of resistance by efflux, which takes place thanks to a transmembrane protein coded by the norA gene and by point mutations (substitution and deletion of acids or nucleotides) observed within the protein and nucleic sequences of the chromosomal gyrA and grLA genes.

关键词： Genes Resistance quinolones/fluoroquinolones Staphylococci

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