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Optimization of the quenching method for metabolomics analysis of Lactobacillus bulgaricus

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第4期15卷 333-342页

作者： Ming-ming CHEN Ai-li LI Mao-cheng SUN Zhen FENG Xiang-chen MENG Ying WANG MOE Key Laboratory of Dairy Science College of Food ScienceNortheast Agricultural University School of Public Health Jilin Medical College

This study proposed a quenching protocol for metabolite analysis of Lactobacillus delbrueckii *** cells were quenched with 60% methanol/water,80% methanol/glycerol,or 80% methanol/*** effect of the quenching process was assessed by the optical density（OD）-based method,flow cytometry,and gas chromatography-mass spectrometry（GC-MS）.The principal component analysis（PCA） and orthogonal partial least squares-discriminant analysis（OPLS-DA） were employed for metabolite *** results indicated that quenching with 80% methanol/water solution led to less damage to the *** cells,characterized by the lower relative fraction of prodium iodide（PI）-labeled cells and the higher OD recovery *** GC-MS analysis,higher levels of intracellular metabolites（including focal glutamic acid,aspartic acid,alanine,and AMP） and a lower leakage rate were detected in the sample quenched with 80% methanol/water compared with the *** conclusion,we suggested a higher concentration of cold methanol quenching for *** metabolomics due to its decreasing metabolite leakage.

关键词： Metabolomics quenching method Lactobacillus bulgaricus Leakage

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Effect of different quenching processes following solid-solution treatment on properties and precipitation behaviors of 7050 alloy

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Transactions of Nonferrous Metals Society of China 2018年第11期28卷 2163-2173页

作者： Lei KANG Gang ZHAO Guang-dong WANG Kun LIU Ni TIAN Key Laboratory for Anisotropy and Texture of Materials Ministry of EducationSchool of Materials Science and EngineeringNortheastern UniversityShenyang 110819China Department of Applied Science University of Quebec at ChicoutimiQuebecG7H 2B1Canada

The effects of quenching in different ways following solid-solution treatment on properties and precipitation behaviors of7050 alloy were investigated by transmission electron microscopy （TEM）, scanning electron microscopy （SEM）, selected areaelectron diffraction （SAED）, hardness and electrical conductivity tests. Results show that after quenching in different ways, electricalconductivity of the alloy decreases rapidly in the first 48 h of natural aging. The electrical conductivity of 7050 alloy in natural agingstate is determined by the size and density of GP zones, and the size of GP zones is the main factor. After natural aging for 70 d, thesize of GP zones is 1.8-2.6 nm in matrix of the immersion quenched sample and it is 1.4-1.8 nm in matrix of both water mist andforced air quenched samples. After natural and artificial peak aging, the hardness of the water mist quenched sample is HV 193.6 andits electrical conductivity is 30.5% （IACS） which are both higher than those of the immersion quenched sample. Therefore, watermist quenching is an ideal quenching method for 7050 alloy sheets after solid-solution treatment.

关键词： 7050 aluminum alloy quenching method precipitation behavior electrical conductivity nucleation site

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