检索结果-南通市图书馆

Establishment and performance analysis of a new multiplex detection method for influenza an and B virus antigen

World Journal of Clinical Cases 2024年第23期12卷 5338-5345页

作者： Cheng-Jing Xia Bao-Hua Li Yan-Ni Guo Xiao-He Zhou Run-Ling Zhang Ying-No Niu Department of Clinical Laboratory West WingShenzhen Hospital(Guangming)of University of Chinese Academy of SciencesShenzhen 518106Guangdong ProvinceChina Laboratory Nanjing Vazyme Biotech Co.LtdNanjing 210033Jiangsu ProvinceChina

BACKGROUND Influenza A and B virus detection is pivotal in epidemiological surveillance and disease *** and accurate diagnostic techniques are crucial for timely clinical intervention and outbreak *** dot-encoded microspheres have been widely used in *** integration of quantum dot-encoded microspheres with flow cytometry is a well-established technique that enables rapid ***,establishing a multiplex detection method for influenza A and B virus antigens based on flow cytometry quantum dot microspheres will help in disease *** To establish a codetection method of influenza A and B virus antigens based on flow cytometry quantum dot-encoded microsphere technology,which forms the foundation for the assays of multiple respiratory virus *** Different quantum dot-encoded microspheres were used to couple the monoclonal antibodies against influenza A and *** known influenza A and B antigens were detected both separately and simultaneously on a flow cytometer,and the detection conditions were optimized to establish the influenza A and B antigen codetection method,which was utilized for their detection in clinical *** results were compared with the fluorescence quantitative polymerase chain reaction(PCR)method to validate the clinical performance of this *** The limits of detection of this method were 26.1 and 10.7 pg/mL for influenza A and B antigens,respectively,which both ranged from 15.6 to 250000 pg/*** the clinical sample evaluation,the proposed method well correlated with the fluorescent quantitative PCR method,with positive,negative,and overall compliance rates of 57.4%,100%,and 71.6%,*** A multiplex assay for quantitative detection of influenza A and B virus antigens has been established,which is characterized by high sensitivity,good specificity,and a wide detection range and is promising for clinical applications.

关键词： Influenza A Influenza B quantum dot microspheres Antigen detection Multiplex detection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：