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赛默飞世尔104亿欧元收购qiagen

食品安全导刊 2020年第10期 17-17页

作者：张聪

本刊讯(记者张聪)3月3日,全球科学服务领导者赛默飞世尔科技联合全球领先的分子诊断和样品制备技术提供商qiagen宣布,双方董事会已一致表决通过,赛默飞以104亿欧元(约115.9亿美元),即每股39欧元现金收购qiagen的提议。该交易预计将于202... 详细信息

本刊讯(记者张聪)3月3日,全球科学服务领导者赛默飞世尔科技联合全球领先的分子诊断和样品制备技术提供商qiagen宣布,双方董事会已一致表决通过,赛默飞以104亿欧元(约115.9亿美元),即每股39欧元现金收购qiagen的提议。该交易预计将于2021年上半年完成。据报道,早在2019年11月,赛默

关键词： qiagen 首席执行官

交流DNA检验心得体会,共享检验成功经验——分享DNA检验成功经验,赢qiagen大奖活动!

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中国法医学杂志 2012年第6期 430-430页

目前,法医DNA检验过程中,遇到痕量样本、污染样本、长时间保存样本等等都会影响到分析检测的准确性和可靠性。针对此类问题,《中国法医学杂志》社与qiagen公司联手举办"疑难检材成功经验的征集和分享活动",并借助《中国法医学杂志》社... 详细信息

目前,法医DNA检验过程中,遇到痕量样本、污染样本、长时间保存样本等等都会影响到分析检测的准确性和可靠性。针对此类问题,《中国法医学杂志》社与qiagen公司联手举办"疑难检材成功经验的征集和分享活动",并借助《中国法医学杂志》社的网络/纸质媒体,给广大法医DNA检验工作者提供一个相

关键词：中国法医学杂志 qiagen 心得体会法医 DNA

Anthropological analyses of 30 insertion/deletion autosomal markers in five major ethnic groups of Pakistan

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Forensic Sciences Research 2022年第2期7卷 202-206页

作者： Muhammad Adnan Shan Julie Mechlenborg Rebecca Roen Claus Bosting Niels Morling Section of Forensic Genetics Department of Forensic MedicineFaculty of Health and Medical SciencesUniversity of CopenhagenCopenhagenDenmark Centre for Applied Molecular Biology(CA MB) University of the PunjabLahorePakistan

We investigated the forensic efficacy of the 30 insertion/deletion(Indel)markers included in the qiagen Investigator■DIPplex kit in 529 Pakistani individuals from five major subpopulations in Pakistan(Punjabi,Pashtun,Sindhi,Saraiki,and Baloch).In the Sindhi population,the distribution of HLD81 and HLD97 alleles deviated from Hardy-Weinberg equilibrium after Bonferroni *** combined match probability ranged from 2.0E-12(Pashtun and Baloch)to 1.0E-12(Sindhi),and the mean paternity exclusion power varied from 0.995(Punjabi,Sindhi,and Saraiki)to 0.996(Pashtun and Baloch).The high combined power of discrimination(0.99999999999997)and low combined match probability(1.7E-12)for all subpopulations studied support the utility of the 30 Indel markers for forensic identification in the studied *** allele frequencies of the Indel markers in the Pakistani subpopulations were compared with those from 18 other *** results show that the populations clustered according to *** subpopulations investigated in this work showed a close genetic relationship with others from Pakistan,as well as with South Central Asian and Middle Eastern *** results suggest that the Investigator■DIPplex kit can be useful as a supplementary tool for human identification in the five Pakistani subpopulations investigated in this study.

关键词： Forensic sciences forensic genetics qiagen investigator■DIPplex kit anthropology population genetics individual identification

不同核酸提取试剂盒对血浆游离DNA提取效果的比较

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国际检验医学杂志 2022年第8期43卷 979-983,989页

作者：方仲表沈伟锋饶焕新章余旋潘志文吴欧浙江树人大学树兰国际医学院浙江杭州310015 中翰盛泰生物技术股份有限公司浙江杭州311100 浙江省肿瘤医院浙江杭州310022

目的比较北京天根生化科技有限公司生产的大体积游离核酸提取试剂盒(磁珠法,货号:DP-710,以下简称天根)与德国qiagen公司生产的Circulating Nucleic Acid kit(硅胶膜吸附柱法,货号:55114,以下简称qiagen)对血浆当中游离DNA(cfDNA)的提... 详细信息

目的比较北京天根生化科技有限公司生产的大体积游离核酸提取试剂盒(磁珠法,货号:DP-710,以下简称天根)与德国qiagen公司生产的Circulating Nucleic Acid kit(硅胶膜吸附柱法,货号:55114,以下简称qiagen)对血浆当中游离DNA(cfDNA)的提取效果。方法收集qiagen和天根试剂盒的基本情况,采用浙江省肿瘤医院提供的临床血浆样本,用qiagen和天根分别提取血浆样本中的cfDNA。用超微紫外分光光度仪分析两种试剂盒提取得到cfDNA的浓度和纯度。采用人表皮生长因子受体(EGFR)突变基因检测试剂盒(多重荧光PCR法)评价两种试剂盒提取血浆中cfDNA的提取效果,通过扩增结果的Ct值分析两种不同试剂盒对人EGFR基因突变的检出率和重复性的影响。结果在cfDNA提取浓度、纯度方面,qiagen的cfDNA提取浓度明显高于天根,且qiagen提取得到的cfDNA纯度更优;在人EGFR突变基因阳性检出率方面,qiagen阳性检出率为100%,天根阳性检出率为85%;在重复性方面,qiagen试剂盒提取的cfDNA的检出结果Ct值的变异系数(CV)均小于5%,而天根试剂盒有分部分CV超过5%。结论qiagen试剂盒在cfDNA提取浓度、纯度,人EGFR突变基因的阳性检出率、重复性方面的表现均要优于天根试剂盒。

关键词：天根 qiagen 核酸提取游离DNA EGFR突变基因

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Low and High Risk Human Papillomavirus in the Oral Mucosa of Mexican Women with Genital Papillomavirus

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Open Journal of Medical Microbiology 2013年第1期3卷 62-69页

作者： Ana María Fernández Dora Rosete Sergio Pedraza Fidencio Mejía José Delgado María Eugenia Manjarrez Experimental Medicine Department Medicine Faculty National Autonomous University of Mexico (UNAM) Mexico City Mexico Microbiology and Parasitology Department Medicine Faculty National Autonomous University of Mexico (UNAM) Mexico City Mexico Spanish Hospital Mexico City Mexico Virology Research Department National Institute of Respiratory Disease (INER) México City Mexico 3Spanish Hospital Mexico City Mexico

Background: Human papillomaviruses (HPV) are implicated in cervical cancer and, recently in oral cancer. In Mexico, there are few studies on oral cancer, therefore the interest in identifying the HPV frequency of low and high risk in samples of the oral and cervical cavities, and determining some risk factors. Objective: To determine the frequency of high and low risk HPV infection in the oral cavity of women with cervical HPV, and to correlate the infection site with risk factors. Materials and Methods: Eighteen female patients between 24 and 53 years, with antecedents of genital HPV infection were included. Both samples of oral cavity and cervix were obtained. DNA extraction from the epithelial cells was performed using the qiagen kit. PCR was done and the amplicon was observed in 2% agarose gels stained with ethidium bromide. A correlation of HPV infection and risk factors was done. Results: HPV-DNA was detected in the 67% of both samples. The frequency of oral and cervix low risk HPV-DNA was 50%, while high risk HPV-DNA in oral cavity was detected in 17%, and 39% in the cervix. The study of the risk factors involved in HPV infection showed that the participants had the habits of smoking 39%;alcohol drinking 28%;and 78% oral sex. Conclusion: The results showed a high frequency of HPV (67%) infection in the oral and genital mucosas, suggesting that patient’s habits could contribute to the infection;the presence of HPV in the oral mucosa may act as reservoir for new HPV infections.

关键词： Human Papillomaviruses (HPV) HPV-DNA qiagen Kit

AmpFISTR Sinofiler快速PCR扩增初探

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中国法医学杂志 2012年第5期27卷 397-398页

作者：王鸿迪董海成于俊峰邵武本溪市公安局刑侦支队辽宁本溪117000 辽宁省公安厅刑事科学技术研究所辽宁沈阳110032

本文实验选择AmpFISTRSinofiler试剂盒作为研究对象，对如何缩短PCR扩增时间进行了初步探讨。1材料与方法1．

关键词：法医物证学 AmpFISTR Sinofile试剂盒 qiagen Fast Cycling PCR试剂盒快速PCR扩增

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