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A major and stable qtl for wheat spikelet number per spike validated in different genetic backgrounds

Journal of Integrative Agriculture 2022年第6期21卷 1551-1562页

作者： DING Pu-yang MO Zi-qiang TANG Hua-ping MU Yang DENG Mei JIANG Qian-tao LIU Ya-xi CHEN Guang-deng CHEN Guo-yue WANG Ji-rui LI Wei QI Peng-fei JIANG Yun-feng KANG Hou-yang YAN Gui-jun WEI Yu-ming ZHENG You-liang LAN Xiu-jin MA Jian State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Ministry of Science and Technology/Triticeae Research InstituteSichuan Agricultural UniversityChengdu 611130P.R.China College of Resources Sichuan Agricultural UniversityChengdu 611130P.R.China College of Agronomy Sichuan Agricultural UniversityChengdu 611130P.R.China UWA School of Agriculture and Environment and The UWA Institute of Agriculture Faculty of ScienceUniversity of Western AustraliaCrawley 6009Australia

The spikelet number per spike(SNS)contributes greatly to grain yield in *** various genes that control wheat SNS is vital for yield *** study used a recombinant inbred line population genotyped by the Wheat55K single-nucleotide polymorphism array to identify two major and stably expressed quantitative trait loci(qtls)for *** of them(***-2SY-2D.1)was reported previously,while the other(***-2SY-7A)was newly detected and further analyzed in this ***-2SY-7A had a high LOD value ranging from 4.46 to 16.00 and explained 10.21-40.78%of the phenotypic ***-2SY-7A was flanked by the markers AX-110518554 and AX-110094527 in a 4.75-cM interval on chromosome arm *** contributions and interactions of both major qtls were further analyzed and *** effect of ***-2SY-7A was successfully validated by developing a tightly linked kompetitive allele specific PCR marker in an F_(2:3) population and a panel of 101 high-generation breeding wheat ***,several genes including the previously reported WHEAT ORTHOLOG OF APO1(WAPO1),an ortholog of the rice gene ABERRANT PANICLE ORGANIZATION 1(APO1)related to SNS,were predicted in the interval of *** summary,these results revealed the genetic basis of the multi-spikelet genotype of wheat line 20828 and will facilitate subsequent fine mapping and breeding utilization of the major qtls.

关键词： yield potential qtl detection qtl validation predicted genes tightly linked KASP marker

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Identification of Putative qtls Associated with Maize Kernel Length Using Double Haploid Lines

Identification of Putative Qtls Associated with Maize Kernel...

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作者： GHULAM AISHIA 华中农业大学

学位级别：硕士

Maize(Zea mays L.) is one of the most prominent cereal crops playing a crucial role in sustaining global food security. In breeding programs, grain size characters including kernel length(KL), kernel width and kernel thickness are importantly considered for the evaluation of kernel weight. The KL was reported to be significantly and positively correlated with the kernel size or/and kernel weight, as well as the final grain yield of maize. In the present study, the haploid lines derived from the intermated B73×Mo17 Syn 10 doubled haploid(IBM Syn10) mapping population were used to identify the putative qtls for KL in different agroecological environments and to understand the stability of qtl across environment. The linkage map, consists of 6540 markers, with an average chromosome length of 1113.6 came were used for qtl mapping. The major findings of the present experiment are summarized below.1. Phenotypic data revealed that maize KL varied significantly under the main effect as well as the interaction of different environments and DH lines. Under all the environments, the average KL of DH lines was quantitatively higher than their parents, suggesting the efficacy of intermating for ten generations. Higher broad sense heritability(0.89) and lower kurtosis as well as scenes values(qtl analysis of IBM Syn10 DH lines revealed thirty six putative qtls(LOD>2.5) associated with KL, among these 12 and 24 qtls were specifically identified in Heilongjiang and Yunnan environments, respectively. All the identified qtls were well distributed over all the 10 maize chromosomes. Seven putative qtls were found on chromosome 1 and 8, six qtls on chromosome 2, and three qtls for each on chromosome 6, 9 and 10. Only one qtl was observed on chromosome 4 as well as chromosome 5, while two

关键词： double haploid maize kernel length qtl detection SNP markers

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