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qcm Aptasensor for Rapid and Specific Detection of Avian Influenza Virus

Open Journal of Applied Biosensor 2013年第4期2卷 97-103页

作者： Luke Brockman Ronghui Wang Jacob Lum Yanbin Li Cell and Molecular Biology Program University of Arkansas Fayetteville USA Center of Excellence for Poultry Science University of Arkansas Fayetteville USA Department of Biological and Agricultural Engineering University of Arkansas Fayetteville USA

There has been a need for rapid detection of Avian Influenza virus (AIV) H5N1 due to it being a potential pandemic threat. Most of the current methods, including culture isolation and PCR, are very sensitive and specific but require specialized laboratories and trained personnel in order to complete the tests and are time-consuming. The goal of this study was to design a biosensor that would be able to rapidly detect AIV H5N1 using aptamers as biosensing material and a quartz crystal microbalance (qcm) for transducing method. Specific DNA aptamers against AIV H5N1 were immobilized, through biotin and streptavidin conjugation, onto the gold surface of qcm sensor to capture the target virus. Magnetic nanobeads (150 nm in diameter) were then added as amplifiers considering its large surface/volume ratio which allows for faster movement and a higher target molecule binding rate. The result showed that the captured AIV caused frequency change, and more change was observed when the AIV concentration increased. The nanobead amplification was effective at the lower concentrations of AIV, however, it was not significant when the AIV concentration was 1 HA or higher. The detection limit of the aptasensor was 1 HAU with a detection time of 1 h. The capture of the target virus on to the surface of qcm sensor and the binding of magnetic nanobeads with the virus was confirmed with electron microscopy. Aptamers have unlimited shelf life and are temperature stable which allows this aptasensor to give much more consistent results specifically for in field applications.

关键词： Aptasensor Avian Influenza qcm Aptamer Nanobeads

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Development of Non-Labeled qcm Biosensor for the Detection of <i>β</i>-Galactosidase: A Comparative Study of Gold and Polystyrene Nanoparticles

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Advances in Nanoparticles 2013年第2期2卷 182-190页

作者： Krishna Pal Singh Manav Kumar Choudhary Iva Chianella Prashant Singh Cranfield Biotechnology Centre Cranfield Health Cranfield University Bedfordshire England Department of Chemistry D.A.V. (PG) College Dehradun H.N.B. Garhwal University Srinagar India Membrane Biophysics & Nanobiosensor Research Laboratory C.B.S H G. B. Pant University of Agriculture& Technology Pantnagar India

The performance of gold and polystyrene nanoparticles was investigated using quartz crystal microbalance (qcm) as sensor platform;β-Galactosidase antibody with corresponding antigen was utilized for the immunoreaction. The development of the immunosensor included: 1) formation of self assembled monolayers on quartz crystals;2a) immobilization of p-aminothiophenol functionalized gold nanoparticles on carboxyl-terminated self assembled monolayer, or 2b) immobilization of polystyrene nanoparticles on crystals modified with p-aminothiophenol self assembled monolayer;3) attachment of monoclonal anti β-Gal on nanoparticles;and 4) detection of target analyte. The nanoparticles used were synthesized in house and characterized by transmission electron microscopy and infrared spectroscopy. The results revealed that antibodies were strongly attached to functionalized gold nanoparticles;the weaker immobilization of antibodies to polystyrene nanoparticles provoked their detachment during antigen detection. When cross reactivity of polystyrene nanoparticles was checked using a different antigen (Brucella), displacement of antibody was not recorded, demonstrating specificity of the reaction. To the best of our knowledge this is the first direct comparison between behaviors of biosensors developed with two commonly used nanoparticles. The results showed that both nanoparticles produced biosensors capable to detect β-Gal. Nevertheless biosensors developed using polystyrene nanoparticles are simpler, cheaper and more eco-friendly than those developed using gold nanoparticles.

关键词： qcm Biosensor β-Galactosidase Gold Nanoparticle Polystyrene Nanoparticle

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沸石分子筛修饰的qcm类神经毒气传感器

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传感技术学报 2006年第5B期19卷 2111-2113,2118页

作者：杨涓涓任楠周嘉张亚红刘全黄宜平唐颐复旦大学微电子学系专用集成电路及系统国家重点实验室上海200433 复旦大学化学系上海200433

研究了Cu2+-Beta型纳米分子筛对类神经毒剂DMMP有机气体的敏感特性,并结合高灵敏的石英谐振微天平(qcm)研制了DMMP气体的传感器.研究结果表明,选择Cu2+-Beta纳米分子筛作为敏感膜对DMMP气体的检测灵敏度大大提高,达到14.4811Hz/lg(C/pp... 详细信息

研究了Cu2+-Beta型纳米分子筛对类神经毒剂DMMP有机气体的敏感特性,并结合高灵敏的石英谐振微天平(qcm)研制了DMMP气体的传感器.研究结果表明,选择Cu2+-Beta纳米分子筛作为敏感膜对DMMP气体的检测灵敏度大大提高,达到14.4811Hz/lg(C/ppm).在0.2ppmDMMP气体浓度下,传感器的响应时间和恢复时间分别为40s和100s,响应达到103Hz.同时经过高温和水汽吹扫脱附处理,该传感器表现了较好的重复性.

关键词： Cu^2+-Beta分子筛 qcm DMMP 气敏传感器

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基于相关性原理qcm频谱分析系统的设计

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传感技术学报 2007年第6期20卷 1298-1302页

作者：蒋海峰张培仁中国科学技术大学自动化系合肥230027

介绍了一种新型的qcm(Quartz Crystal Microbalance)频谱分析系统的设计,它具有结构简单,实时性好,抗干扰性强,应用领域广,精度高等特点.文中对最小阻抗频率等同谐振频率的合理性做了分析,提出了基于相关性原理采集qcm微弱有用信号的方... 详细信息

介绍了一种新型的qcm(Quartz Crystal Microbalance)频谱分析系统的设计,它具有结构简单,实时性好,抗干扰性强,应用领域广,精度高等特点.文中对最小阻抗频率等同谐振频率的合理性做了分析,提出了基于相关性原理采集qcm微弱有用信号的方法,设计了qcm频谱分析系统的结构.最后进行了液体测量实验,实验结果与理论值基本一致,从而证实了此设计的正确性.

关键词： qcm 最小阻抗频率频谱分析相关性原理

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磺胺功能化SBA-15修饰的qcm气体传感器检测甲醛的研究

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黑龙江大学自然科学学报 2016年第6期33卷 783-788页

作者：袁宇陈杨徐甲强程知萱上海大学材料科学与工程学院上海200444 上海大学化学系新能源材料科学与技术实验室上海200444

采用后合成方法设计合成了一种具有规则六方形貌、高度有序结构的磺胺功能化SBA-15气敏材料，对合成材料进行表征发现：磺胺功能化SBA-15保持着主体材料SBA-15的规则形貌，并制备成敏感膜涂覆于石英晶体微天平（Quartz crystal microbal... 详细信息

采用后合成方法设计合成了一种具有规则六方形貌、高度有序结构的磺胺功能化SBA-15气敏材料，对合成材料进行表征发现：磺胺功能化SBA-15保持着主体材料SBA-15的规则形貌，并制备成敏感膜涂覆于石英晶体微天平（Quartz crystal microbalance，qcm）表面构成qcm甲醛传感器。实验表明，由磺胺功能化SBA-15敏感膜修饰的qcm传感器对甲醛气体有快速可逆的响应（响应时间为5-10s，恢复时间为10-15s）、较好的重现性、较长的使用寿命，相对于甲醇、乙醇等VOCs，显示出较好的选择性。

关键词：有机-无机杂化材料介孔材料磺胺功能化 qcm 甲醛传感器

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Application of a Quartz Crystal Microbalance (qcm) System Coated with Chromatographic Adsorbents for the Detection of Olive Oil Volatile Compounds

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Journal of Sensor Technology 2011年第1期1卷 1-8页

作者： María E. Escuderos Sebastián Sánchez Antonio Jiménez 不详

A sensor based on the technique of a piezoelectric quartz crystal microbalance (qcm) is analyzed for the detection of six organic volatile compounds with high olive oil sensory significance, such as hexanal, acetic acid, Z-3-hexenyl acetate, undecane, 1-octen-3-ol and 2-butanone. Four sample concentrations have been exposed to each qcm sensor constructed. The detection system is based on the sample adsorption on the forty sensing films coated at the surfaces of forty AT-cut gold-coated quartz crystals. Each sensing film has been prepared with different solution concentrations of ten materials, usually used as chromatographic sta-tionary phases. Sensing film coating process shows excellent repeatability, with coefficient values less than 0.50%. The frequency shifts of the piezoelectric crystals due to the adsorption of the volatile compounds have been measured as sensor responses, using a static measurement system. The results show that only five qcm sensors, with high sensitivity values, are enough to the detection of the volatile compounds studied. Therefore, the developed detection system presented herein provides a rapid identification of organic volatile compounds with elevated olive oil sensory connotation and it could be a substitute technique to the analytical methods normally used for the analysis of the olive oil flavor.

关键词： qcm Gas Sensor Chromatographic Adsorbents Olive Oil Volatile Compounds Sensory Connotations Electronic Nose

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The study of gold nanorings used in LSPR sensor combined with qcm

The study of gold nanorings used in LSPR sensor combined wit...

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中国真空学会2014学术年会

作者： Jin Zhu Xiaoqing Zhang Shun Huang Guohua Liu College of Electronic Information and Optic Engineering Nankai University

The LSPR is an optical phenomenon generated by collective oscillations of the electron gas inmetal nanostructures surrounded by a *** LSPR spectral position is highly dependent on the size or shape of *** gold nanoring arrays are used in LSPR sensors due to their near-infrared resonance wavelength demonstrated with high *** use the discrete dipole approximation method to study the extinction spectra of different shape of gold nanorings and the relationship between the extinction peak redshift and the parameter of gold *** qcm is used to measure the amount of added mass to the electrodes which is linearly related to the resulting shift in resonant *** develop a new kind of sensor combined with LSPR and qcm *** sensor presents a good sensitivity in liquids of different dielectric constant.

关键词： nanotechnology nanorings LSPR qcm extinction spectra

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Finite Element Analysis on Electrode Structure of qcm

Finite Element Analysis on Electrode Structure of QCM

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2009中国控制与决策会议

作者： Haifeng Jiang Institute of Automation,NanJing University of Science and Technology

＜正＞Because quartz crystal is complex at high frequency vibration,the only vibration mode of qcm will be destroyed at some designs of electrode structures and couplings with other vibration modes will happen *** paper makes a 3D model for qcm and analyzes directly the coupled phenomenon of the structural and electric fields of qcm with finite element *** analyzing the convergence of the resonant frequency of qcm,the correctness of finite element analysis is *** the influence of different sizes of electrode on vibration of qcm and an optimal size of electrode is *** design of mesa on crystal plate is provided to solve the question that energy trapping is not good *** design proves valid based on finite element calculation and the optimal height of mesa is given.

关键词： qcm coupling finite element energy trapping

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Effect of Electrode Surface Modification by Sulfide on qcm Based Protein Biosensor

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Optics and Photonics Journal 2013年第2期3卷 305-307页

作者： Yu-Cheng Lin Yi-Chi Chen Liang-Yu Chen Department and Graduate Institute of Biological Technol Ming Chung University Taoyuan Chinese Taipei Department and Graduate Institute of Electronic Eng Ming Chung University Taoyuan Chinese Taipei

The rapid development of surface sensitive biosensor technologies requires optimum control of surface modification to provide reliable and reproducible results. With the aim to assemble a quartz crystal microbalance (qcm)-based protein biosensor, we focus our attention on sulfide receptor and its integration with the surface of the electrode. Here, we present different surface modification processing time to allow sulfide molecules to be immobilized to gold coated sensor for qcm sensing. The optimum surface modification processing time is also obtained by bovine serum albumin (BSA) binding measurement.

关键词： Biosensor qcm Protein Binding Surface Modification

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qcm传感器性能的有限元仿真分析

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东北师大学报（自然科学版） 2018年第3期50卷 100-104页

作者：王娇杨王连明东北师范大学物理学院吉林长春130024

由于qcm传感器频率高、振幅小的振动难以观察,无法分析电极对传感器性能的影响.针对这一问题,通过引入了通用有限元分析软件ANSYS,在Workbench平台上建立石英晶片厚度剪切振动模式模型,分析了不同尺寸电极对qcm传感器性能的影响.仿真结... 详细信息

由于qcm传感器频率高、振幅小的振动难以观察,无法分析电极对传感器性能的影响.针对这一问题,通过引入了通用有限元分析软件ANSYS,在Workbench平台上建立石英晶片厚度剪切振动模式模型,分析了不同尺寸电极对qcm传感器性能的影响.仿真结果表明:电极直径增加会导致石英晶体的谐振频率下降;电极尺寸过大过小都容易导致寄生模干扰加强.

关键词：压电效应 qcm 厚度剪切振动 ANSYS

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