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Genome-wide identification of the CONSTANS-LIKE(COL)family and mechanism of fruit senescence regulation by PpCOL8 in sand pear(pyrus pyrifolia)

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Journal of Integrative Agriculture 2024年第4期23卷 1222-1237页

作者： Yue Xu Shurui Song Huiying Wang Xilong Cao Xinran Zhao Wenli Wang Liyue Huo Yawei Li Misganaw Wassie Bin Lu Liang Chen Haiyan Shi College of Horticulture Hebei Agricultural UniversityBaoding 071001China Wuhan Botanical Garden Chinese Academy of SciencesWuhan 430074China

pyrus pyrifolia Nakai‘Whangkeumbae'is a sand pear fruit with excellent nutritional quality and ***,the industrial development of pear fruit is significantly limited by its short shelf *** acid(SA),a well-known phytohormone,can delay fruit senescence and improve shelf ***,the mechanism by which SA regulates CONSTANS-LIKE genes(COLs)during fruit senescence and the role of COL genes in mediating fruit senescence in sand pear are poorly *** this study,22 COL genes were identified in sand pear,including four COLs(Pp COL8,Pp COL9a,Pp COL9b,and Pp COL14)identified via transcriptome analysis and 18 COLs through genome-wide *** COL genes were divided into three subgroups according to the structural domains of the COL *** COL8,with two B-box motifs and one CCT domain,belonged to the first *** contrast,the other three Pp COLs,Pp COL9a,Pp COL9b,and Pp COL14,with similar conserved protein domains and gene structures,were assigned to the third *** four COLs showed different expression patterns in pear tissues and were preferentially expressed at the early stage of fruit ***,the expression of Pp COL8 was inhibited by exogenous SA treatment,while SA up-regulated the expression of Pp COL9a and Pp ***,Pp COL8 interacts with Pp MADS,a MADS-box protein preferentially expressed in fruit,and SA up-regulated its *** the production of ethylene and the content of malondialdehyde(MDA)were increased in Pp COL8-overexpression sand pear fruit,the antioxidant enzyme(POD and SOD)activity and the expression of Pp POD1 and Pp SOD1 in the sand pear fruits were down-regulated,which showed that Pp COL8 promoted sand pear fruit *** contrast,the corresponding changes were the opposite in Pp MADS-overexpression sand pear fruits,suggesting that Pp MADS delayed sand pear fruit *** co-transformation of Pp COL8 and Pp MADS also delayed sand pear fruit *** re

关键词： pyrus pyrifolia CONSTANS-LIKE gene salicylic acid fruit senescence MADS

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Relationship Between Anthocyanin Biosynthesis and Related Enzymes Activity in pyrus pyrifolia Mantianhong and Its Bud Sports Aoguan

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Agricultural Sciences in China 2008年第11期7卷 1318-1323页

作者： FENG Shou-qian CHEN Xue-sen ZHANG Chun-yu LIU Xiao-jing LIU Zun-chun WANG Hai-bo WANG Yan-ling ZHOU Chao-hua State Key Laboratory of Crop Biology Shandong Agricultural University Tai＇an 271018 P.R. China Henan Institute of Science and Technology Xinxiang 453003 P.R.China Liaocheng Forestry Bureau Liaoeheng 252000 P.R.China

The aim of this article is to study the relationship between biosynthesis of anthocyanin and activities of phenylalanine ammonia lyase （PAL）, chalcone ismoerase （CHI） enzymes in pyrus pyrifolia. Changes in the level of anthocyanin and the activities of enzymes of anthocyanin biosynthesis including PAL, CHI were studied in the pericarp of pyrus pyrifolia Aoguan and Mantianhong during the period of pigment formation. Bagging treatment was also carried out to manipulate the synthesis of anthocyanin and the activities of related enzymes during the period of pigment formation. The results demonstrated that the level of anthocyanin of Aoguan was higher than that of Mantianhong. However, the content of anthocyanins has the similar changing trend in Aoguan and Mantianhong, highest anthocyanin concentrations of two varieties appeared in immature fruit and faded toward harvest. Meanwhile, similar changing trends of activities of PAL and CHI were also observed in both varieties. Aoguan has a lower activity of PAL than Mantianhong, whereas activity of CHI in Aoguan was higher than that in Mantianhong. Activity of PAL decreased during the period of pigment formation and was apparently not limiting to color development, whereas CHI activity increased at the same period and was closely related to the synthesis of anthocyanin. The results of bagging treatment showed that bagging treatment inhibited the activity of CHI, as well as the synthesis of anthocyanin, whereas debagging enhanced both the activity of CHI and synthesis of anthocyanin. The activity of CHI in debagging Aoguan pericarp was higher than the untreated Aoguan. However, effect of bagging treatment toward PAL activity was not obvious. Anthocyanin of bagging treated Aoguan decreased toward harvest. The content of anthocyanin of pyruspyrifolia increased at the beginning of fruit coloration period and decreased toward fruit harvest. Activity of PAL was apparently not limiting to color development, whereas CHI activity was

关键词： pyrus pyrifolia anthocyanin PAL CHI

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Screening for resistance to Alternaria alternata,the causal agent of black spot,in pyrus pyrifolia

Screening for resistance to Alternaria alternata,the causal ...

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Yang Xiaoping He Xiujuan Chen Qiliang Zhang Jingguo Fan Jing Hu Hongju Liu Pu Research Institute of Fruit and Tea Hubei Academy of Agricultural Science Hubei Laboratory of Crop Diseases Insect Pests and Weeds Control College of Horticulture Anhui Agricultural University

Black spot disease（BSD）,caused by Alternaria alternata,is one of the most serious fungal disease in sand pear（pyrus pyrifolia）,reducing the productivity and quality of *** is characterized by necrosis on leaves,twigs and *** this study,331 sand pear germplasm including72 improved cultivars,18 breeding lines and 241 landraces from the germplasm bank of WCSPGNFG were evaluated for their resistance to *** under both natural infection and artificial inoculation conditions in three consecutive *** showed that,nine sand pear varieties were highly resistant to ***;Whereas,71 were resistant,165 were moderately resistant,68 were susceptible and 18 were highly susceptible to *** linear stepwise regression analysis indicated that susceptibility of improved cultivars,breeding line and landraces of sand pear to *** was related to five main botanical and biological characteristics:stalk length,branching ability,days of fruit development,weight per fruit,and soluble solids *** results will be helpful for genetic improvement to develop cultivars resistant to pear black spot.

关键词： pyrus pyrifolia Pear black spot Inoculation Resistance

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利用PCR-RFLP技术鉴定部分沙梨（pyrus pyrifolia Nakai）品种S基因型

利用PCR-RFLP技术鉴定部分沙梨（Pyrus Pyrifolia Nakai）品种S基...

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作者：辜青青华中农业大学

学位级别：硕士

梨是世界及我国主栽果树之一，绝大多数品种自花授粉不能结实，相同S基因型品种间异花授粉也不能结实，在生产上必须合理配置授粉树才能获得目标产量和果实品质。自交不亲和S基因型的确定，对于沙梨授粉品种选择及其育种亲本选配具有重... 详细信息

梨是世界及我国主栽果树之一，绝大多数品种自花授粉不能结实，相同S基因型品种间异花授粉也不能结实，在生产上必须合理配置授粉树才能获得目标产量和果实品质。自交不亲和S基因型的确定，对于沙梨授粉品种选择及其育种亲本选配具有重要意义。然而，中国沙梨品种的S基因型鉴定却很少报道。利用S基因特异性PCR-RFLP技术，对23个沙梨品种的S基因型进行了鉴定。结果表明： 1．采用CTAB法提取的沙梨基因组总DNA，大部分OD／OD在1.8左右，产率在200μg／g鲜重叶片以上，RNA去除干净，DNA无降解，能够用于本试验的后续分子生物学实验分析;2．5个S基因型已知的日本沙梨品种，其鉴定结果与已知相符;3．采用S基因特异性PCR-RFLP技术鉴定了18个未知S基因型沙梨品种的S基因型，其结果如下：金水1号(SS)、金水2号(SS)、江岛(SS)、华梨1号(SS)、湘南(S1)、翠冠(SS4S)、杭青(S3)、西子绿(SS)、新杭(SS)、清香(SS)、三花(SS)、黄蜜(今村夏)(SS)、黄花(SS)、二宫白(SS)、华梨2号(SS)、宝珠梨(SS)、苍溪雪梨(SS)、灌阳雪梨(SS);4．通过亲本S基因型分析，9个中国杂交沙梨品种，除华梨1号外，其余8个通过PCR-RFLP鉴定的S基因型均与亲本S基因型相符;所鉴定的华梨1号S基因型与其母本湘南同为SS，田间授粉试验结果也证明其异交不亲和;5．通过F代S基因型分析，初步确定三花S为S，则三花S基因型为SS;6．田间授粉试验结果表明，S基因型相同的华梨2号(SS)与新世纪(SS)异交，表现为不亲和，说明利用PCR-RFLP鉴定中国沙梨品种S基因型是准确可行的，通过本试验鉴定的华梨2号S基因型是正确的

关键词： pyrus pyrifolia 自交不亲和 S基因型 PCR-RFLP

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Involvement of long non-coding RNAs in pear fruit senescence under high-and low-temperature conditions

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Horticultural Plant Journal 2023年第2期9卷 224-236页

作者： Yuhang Zhou Xueping Wang Kaijie Qi Jianping Bao Shaoling Zhang Chao Gu Centre of Pear Engineering Technology Research State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjing210095China College of Plant Science Tarim UniversityAlaer843300XinjiangChina

Pear fruit senescence under high-and low-temperature conditions has been reported to be mediated by *** non-coding RNAs(lncRNAs),which can function as competing endogenous RNAs that interact with microRNAs,may also be involved in temperature-affected fruit *** on the transcriptome and microRNA sequencings,in this study,3330 lncRNAs were isolated from pyrus pyrifolia *** these lncRNAs,2060 and 537 were responsive to high-and low-temperature conditions,*** these differentially expressed lncRNAs,82 and 24 correlated to the mRNAs involved in fruit senescence under high-and low-temperature conditions,***,three lncRNAs were predicted to be competing endogenous RNAs(ceRNAs)that interact with the microRNAs involved in fruit senescence,while one and two ceRNAs were involved in fruit senescence under high-and low-temperature conditions,respectively.A dual-luciferase assay showed that the interaction of an lncRNA with a microRNA disrupts the action of the microRNA on the expression of its target mRNA(s).Furthermore,four alternative splicing-derived lncRNAs interacted with miR172i homologies(Novel_88 and Novel_69)to relieve the repressed expression of their target and produce an miR172i *** analysis of microRNA expression suggested that Novel_69 is likely involved in the cleavage of the pre-miR172i hairpin to generate mature *** together,lncRNAs are involved in pear fruit senescence under high-or low-temperature conditions through ceRNAs and the production of microRNA.

关键词： pyrus pyrifolia Long non-coding RNA(lncRNA) Fruit senescence High-temperature Low-temperature lncRNA-microRNA-mRNA interaction

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Distribution of Apple Stem Grooving Virus and Apple Chlorotic Leaf Spot Virus in Infected in vitro Pear Shoots

Distribution of Apple Stem Grooving Virus and Apple Chloroti...

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中国植物病理学会第九届全国会员代表大会暨2010年学术...

作者： Wang Lingping~(**),Wang Guoping,Hong Ni~(***) (The Key Laboratory of Plant Pathology of Hubei Province,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,People''s Republic of China)

＜正＞Apple stem grooving virus（ASGV） and Apple chlorotic leaf spot virus（ACLSV） are two important viruses in pear,which are worldwide *** commercially cultivated pear,these two viruses usually do not cause visible symptoms,but they decrease the growth and productivity of infected *** of most effective measures for the controlling of these virus diseases is utilizing certified healthy propagation materials. Thermotherapy was the most widely used measure for the production of virus-free plant *** this study, for obtaining higher virus elimination efficiency,the distribution features of Apple stem grooving virus（ASGV） and Apple chlorotic leaf spot virus（ACLSV） in in vuro-cultured pear plants was investigated by using in situ tissue-printing hybridization（TPH） and tissue blotting immunoassay（TBIA） to detect viral RNAs and coating *** ASGV and ACLSV showed high concentrations in the tip of the pear plants and lower concentrations in the middle *** highest viral RNA titers were found in the phloem parenchyma of vascular *** of viral RNA concentrations was conducted on infected in vitro-cultured pear plants during heat-treated periods of 50 days at 34℃/42℃（day/night regime of 16 h light at 42℃,and 8 h dark at 34℃） by using TPH combined with X-ray film exposure in serial cross *** viral RNA was detected in tips of 2 mm and 0.5 mm *** heat treatment was less effective to reduce virus titers in the bottom *** distribution of ACLSV and ASGV at viral RNAs and proteins levels in the whole in vitro-cultured pear shoots before and after thermotherapy was *** result would assist in the selection of in vitro-cultured plant parts with low virus concentration to be used for thermotherapy,and improving the selection meristem tips of proper sizes of in vitro cultured pear shoots for sub-culturing thus ensuring complete elimination of ACLSV and ASGV efficiently for micro-propagation.

关键词： Apple cholortotic leaf spot virus Apple stem grooving virus pyrus pyrifolia Thermotherapy

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