检索结果-南通市图书馆

Electrophysiologic Effects of Propafenone on Action Potential of Neonatal and Adult purkinje Fibers

中国医科大学学报 1991年第S2期20卷 37-42页

作者：徐惠郝丽英张克仪 Arthur S.Pickoff Adrienne Stolfi Dept of Pediatrics The First Affiliated Hospital China Medical University Shenyang China Department of Pediatrics Tulane University Medical Center USA M.D. Department of Pediatrics Tulane University Medical Center USA

The electrophysiological effects of 5.8×10-6 mol/L propafenonewere studied in neonatal canine purkinje fiber compared with changes in theadult canine. The method used was microelectrode technique. This study sug-gests that purkinje fibers are less sensitive to propafenone in the neonate than inthe adult, but at shorter ample lengths, the difference between them is not sig-nificant.

关键词： propafenone action potential purkinje fibers

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

purkinje-neuron-specific down-regulation of p38 protects motoric function from the repeated use of benzodiazepine

引用

Advances in Bioscience and Biotechnology 2013年第6期4卷 61-71页

作者： Marianna Jung Daniel Metzger Departments of Pharmacology and Neuroscience University of North Texas Health Science Center Fort Worth USA

Benzodiazepine (BZD) is the most prescribed CNS depressant in America to treat hyper-excitatory disorders such as anxiety and insomnia. However, the chronic use of BZD often creates adverse effects including psychomotor deficit. In this study, we investigated a novel mechanism by which chronic BZD impedes motoric function in female mice. We used female mice because BZD use is much more prevalent in female than male populations. We tested the hypothesis that the accumulation of p38 (stress-activated protein) in cerebellar purkinje neurons mediates motoric deficit induced by chronic BZD. To test this hypothesis, we generated transgenic mice that lack p38 incerebellar purkinje neurons by crossing Pcp2 (purkinje cell protein 2)-Cre mice with p38loxP/loxP mice. p38-knockdown mice and wild-type mice received BZD (lorazepam, 0.5 mg/kg) for 14 days. During this period, they were tested for motoric performance using Rotarod assay in which a quicker fall from rotating rod indicates poorer motoric performance. Cerebellum was then collected to detect p38 inpurkinje neurons and to measure mitochondrial respiration using immunohistochemistry and real-time XF respirometry, respectively. Compared to vehicletreated mice, BZD-treated mice showed poorer motoric performance, a higher number of purkinje neurons containing p38, and lower mitochondrial respiration. These effects of BZD were much smaller in p38-knockdown mice. These results suggest that the excessive accumulation of p38 incerebellar purkinje neurons contributes to motoric deficit associated with chronic BZD. They also suggest that purkinje neuronal p38 mediates BZD-induced mitochondrial respiratory inhibition in cerebellum. Our findings may provide a new mechanistic insight into chronic BZD-induced motoric deficit.

关键词： Benzodiazepine Motoric Deficit p38 purkinje Neurons Mitochondria

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Contribution of plasma membrane Ca^(2+) ATPase to cerebellar synapse function

引用

World Journal of Biological Chemistry 2010年第5期1卷 95-102页

作者： Helena Huang Raghavendra Y Nagaraja Molly L Garside Walther Akemann Thomas Knpfel Ruth M Empson Department of Physiology Brain Health and Repair Research CentreUniversity of Otago School of Biological Sciences Royal Holloway University of London Laboratory for Neuronal Circuit Dynamics RIKEN Brain Science Institute

The cerebellum expresses one of the highest levels of the plasma membrane Ca2+ATPase,isoform 2 in the mammalian brain.This highly efficient plasma membrane calcium transporter protein is enriched within the main output neurons of the cerebellar cortex;i.e. the purkinje neurons(PNs) .Here we review recent evidence,including electrophysiological and calcium imaging approaches using the plasma membrane calcium ATPase 2(PMCA2) knockout mouse,to show that PMCA2 is critical for the physiological control of calcium at cerebellar synapses and cerebellar dependent behaviour.These studies have also revealed that deletionof PMCA2 throughout cerebellar development in the PMCA2 knockout mouse leads to permanent signalling and morphological alterations in the PN dendrites. Whilst these findings highlight the importance of PMCA2 during cerebellar synapse function and development,they also reveal some limitations in the use of the PMCA2 knockout mouse and the need for additional experimental approaches including cell-specific and reversible manipulation of PMCAs.

关键词： Plasma membrane Ca2+ ATPase Cerebellum Calcium purkinje neuron

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Immunolocalization of Ephexin-1 in the Developing Canine Cerebellum

引用

Neuroscience & Medicine 2014年第1期5卷 9-14页

作者： Hyun Sik Park In Youb Chang Han Yong Kim Sang Pil Yoon Department of Anatomy College of Medicine Chosun University Gwangju Republic of Korea Department of Anatomy School of Medicine Jeju Na-tional University Jeju City Republic of Korea Department of Plastic Surgery College of Medicine Seonam University Namwon Republic of Korea Medical Course School of Medicine Jeju National University Jeju City Republic of Korea

Ephexin-1 functions as guanine nucleotide exchange factors for the Rho-type GTPases which have important roles in neuronal development including axon guidance, migration, morphogenesis, and plasticity of neurons. As little is known about ephexin-1 in the cerebellum, we investigated the immunolocalization of ephexin-1 in the developing canine cerebellum. While the cellular maturation was followed by the temporal pattern, the calbindin D-28k and ephexin-1 immunoreactivities gradually increased in developing canine cerebellum. When compared to the calbindin D-28k immunoreactivities, belated ephexin-1 immunolocalization was observed in the purkinje cells which aligned a single layer during cerebellar development. These results suggest that ephexin-1 might play an important role in the development of the purkinje cells during the first two postnatal weeks based on its immunolocalization in the present study.

关键词： Ephexin-1 Cerebellum purkinje Cells Development Dog

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

国人胎儿小脑皮质purkinje细胞层突触的发育

引用

解剖科学进展 2000年第1期6卷 95-95页

作者：张国境田向春沈瑞莲邹超北京医学高等专科学校组胚教研室

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

用卢戈(Lugol)碘液显示心传导系统的浦肯野氏(purkinje)纤维

引用

西安交通大学学报(医学版) 1987年第2期 147-147页

作者：孔祥云西安医科大学人体解剖学教研室

利用心传导系统中的浦肯野氏纤维糖原含量较高的特点,而浦氏纤维仅位于薄层心内膜下,故用卢戈碘液涂布在心膜上,使碘液与糖原结合,产生棕褐色的颜色反应,这种染色法简便易行,能充分地显示出心内膜下浦氏纤维细网,对教学与科研都有一定... 详细信息

利用心传导系统中的浦肯野氏纤维糖原含量较高的特点,而浦氏纤维仅位于薄层心内膜下,故用卢戈碘液涂布在心膜上,使碘液与糖原结合,产生棕褐色的颜色反应,这种染色法简便易行,能充分地显示出心内膜下浦氏纤维细网,对教学与科研都有一定实用意义。其操作方法如下:1.取材法:要用死后1～4小时以内的新生儿心脏或动物心脏(牛、狗、猪心),

关键词：卢戈心传导系统 purkinje 碘液 Lugol

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：