检索结果-南通市图书馆

Studies on Properties of Lipase Produced from Aspergillus sp. Isolated from Compost Soil

Advances in Enzyme Research 2022年第2期10卷 49-60页

作者： Chijioke O. Ezenwelu Oladejo A. Afeez Obiano U. Anthony Okoro A. Promise Ude-Ezika C. Mmesoma Oparaji E. Henry Department of Applied Biochemistry Nnamdi Azikiwe University Awka Anambra State Nigeria Department of Chemical Sciences Spiritan University Nneochi Abia State Nigeria

Lipase producing Aspergillus was isolated from soil collected from a refuse dump site located at Awka, Anambra State, Nigeria using standard microbiology and biochemical techniques. Crude extract of lipase was produced after a successful screening of the isolates using mineral broth containing p-NPP through submerged fermentation system with optimized physiologic conditions. Three steps of purification were carried out: Ammonium sulphate, dialysis and gel filtration (sephadex G-150). Crude extract was precipitated by using 70% saturation of ammonium sulphate at pH 6.0 which gave the optimum precipitation of the protein with specific activity of 260.56 U/mg. Precipitation using ammonium sulphate carried out at pH 6.5 and 8.0 gave specific activity of 217 U/mg of the protein. The precipitates were further desalted through dialysis for twelve hours and specific activity of 343.20 U/mg was recorded from the dialysate afterwards. Further purification was done by using sephadex G-150 and specific activity of 490.55 U/mg was recorded from the active pooled fractions. The purification table showed a 2.32 purification folds of lipase was gotten after gel filtration (sephadex G-150) with a lipase percentage yield of 2.00%. The specific activity of lipase increased from 211.81 to 490.55 U/mg. Characterization of β-galactosidase gave optima pH and temperature of the enzyme at 6.0 and 60°C respectively. Kinetic constants: Km and Vmax values were obtained at various concentrations of p-NPP where 0.32 mM and Vmax of 200.00 μmol/min respectively. Ca2+ and Co2+ showed greater effect on lipase activity in a concentration-dependent manner (0.03 - 0.05 M) when compared to Mn2+ and Fe2+. The results from this study have shown that lipase produced from filamentous Aspergillus has a wide range of activity over physiologic conditions in regards to industrial and clinical standard operational procedures.

关键词： Lipase purification Specific Activity Precipitation purification folds

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：