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中国畜牧兽医学会家畜内科学分会2009年学术研讨会

作者：潘家强 Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls Nanjing Agricultural University

pulmonary artery endothelial cells(PAEC) of broiler were isolated by the method of tissue *** of the cells were performed with the morphological feature and immunocytochemical stain using specific antiserum against factorⅧrelated ***/Xanthine oxidase(X/XO) served as the oxygen free radical(OFR) generating *** vitro model of oxidative injury of PAEC was established based on the X/XO *** effect of OFR on the growth and viability of PAEC was determined with methylthiazol tetrazolium(MTT) colorimetric *** (MDA,a product of lipid peroxidation) in culture medium of PAEC was detected by a thiobarbituric acid colorimetric *** result showed that PAEC could be survived in vitro and had been subcultured for 5-6 *** and immunohistochemical observing of cultured cells demonstrated specific characteristics of endothelial *** were severely damaged by *** viability of cells was inhibited by X/XO system and dose-dependent decrease of cell viability was found with increased XO *** promoted the lipid peroxidation of PAEC and increased the MDA concentration in culture *** results suggest that OFR had an injury effect on the endothelial cells of pulmonary artery in broiler,which was consistent with the result in vivo,and may play an important role in the pathogenesis of pulmonary hypertension syndrome in broiler.

关键词： Broiler pulmonary artery endothelial cells In vitro culture Oxygen free radical

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Bench to beside:the imbalance of mutual regulation of homocysteine and hydrogen sulfide in congenital heart disease related pulmonary arterial hypertension

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South China Journal of Cardiology 2022年第1期23卷 39-52页

作者： XU Yu-kai SUN Ling JIANG Qiu-ping XIE Yu-Mei WANG Shu-shui ZHANG Zhi-wei Guangdong Cardiovascular institute Guangdong Provincial People's HospitalGuangdong Academy of Medical SciencesGuangzhou 510080Guangdong ProvinceChina Department of Pediatric Cardiology Guangdong Provincial People's HospitalGuangdong Academy of Medical SciencesGuangdong Cardiovascular instituteGuangdong Provincial Key Laboratory of South China Structural Heart Disease

Background To determine the imbalance of mutual regulation of homocysteine and hydrogen sulfide(H;S)in congenital heart disease(CHD)-related pulmonary arterial hypertension(PAH)among pediatric patients,and explore possible *** and Principal Findings:In this study,we regulated homocysteine concentrations to observe the relations between homocysteine and H;*** viability and activity of metabolic enzymes were *** experiments demonstrated that exogenous or endogenous H;S both had protective effects on HPAECs and can inhibit homocysteine-induced *** possible mechanisms were correlated with GRP78 and CHOP expressions of endoplasmic reticulum stress *** addtion,we found that homocysteine and H;S were in a dynamic change,which was related to the homocysteine *** the homocysteine concentrations were low(≤30μmol/L),the protective effects of H;S can resist the homocysteine-induced damage ***,the cytological results were different from the clinical *** clinical study had showed that the levels of homocysteine were higher,the levels of H;S and the OD values of cystathionine gamma-lyase(CSE)were lower in the PAH *** the CHD-PAH patients had low homocysteine(≤30μmol/L)concentrations still lead to PAH because of decreased the protective effects of H;S due to the decreased activity of ***:Homocysteine and H;S both take part in the development of *** may be the pathogenic factor,while H;S is the protective *** mutual dynamic regulations are related to the homocysteine *** clinical trials and cytological experiment results have great implications for clinical *** patients with PAH,not only the damage of homocysteine to endothelial cells,but also we should pay attention to the decreased protection of H;S and activity of metabolic enzymes.

关键词： pulmonary arterial hypertension homocysteine hydrogen sulfide pulmonary artery endothelial cells endoplasmic reticulum stress

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