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The involvement of proline-rich protein Mus musculus predicted gene 4736 in ocular surface functions

International Journal of Ophthalmology(English edition) 2016年第8期9卷 1121-1126页

作者： Xia Qi Sheng-Wei Ren Feng Zhang Yi-Qiang Wang State Key Laboratory Cultivation Base Shandong Provincial Key Laboratory of Ophthalmology Shandong Eye InstituteShandong Academy of Medical Sciences Linyi People's Hospital Henan Eye Institute & Henan Eye Hospital Eye Department of Henan People's Hospital People's Hospital of Zhengzhou University MOH Key Lab of Thrombosis and Hemostasis Collaborative Innovation Center of Hematology Jiangsu Institute of Hematology the First Affiliated Hospital of Soochow University

AIM: To research the two homologous predicted proline -rich protein genes, Mus musculus predicted gene 4736 (MP4) and proline-rich protein BstNI subfamily 1 (Prb1) which were significantly upregulated in cultured corneal organs when encountering fungal pathogen preparations. This study was to confirm the expression and potential functions of these two genes in ocular surface. METHODS: A pseudomonas aeruginosa keratitis model was established in Balb/c mice. One day post infection, mRNA level of MP4 was measured using real-time polymerase chain reaction (PCR), and MP4 protein detected by immunohistochemistry (IHC) or Western blot using a customized polyclonal anti -MP4 antibody preparation. Lacrimal glands from normal mice were also subjected to IHC staining for MP4. An online bioinformatics program, BioGPS, was utilized to screen public data to determine other potential locations of MP4. RESULTS: One day after keratitis induction, MP4 was upregulated in the corneas at both mRNA level as measured using real -time PCR and protein levels as measured using Western blot and IHC. BioGPS analysis of public data suggested that the MP4 gene was most abundantly expressed in the lacrimal glands, and IHC revealed that normal murine lacrimal glands were positive for MP4 staining. CONCLUSION: MP4 and Prb1 are closely related with the physiology and pathological processes of the ocular surface. Considering the significance of ocular surface abnormalities like dry eye, we propose that MP4 and Prb1 contribute to homeostasis of ocular surface, and deserve more extensive functional and disease correlation studies.

关键词： proline-rich protein Mus musculus predicted gene 4736 ocular surface pseudomonas aeruginosa keratitis

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