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Microbiologically influenced corrosion of titanium caused by aerobic marine bacterium pseudomonas aeruginosa

Journal of Materials Science & Technology 2019年第1期35卷 216-222页

作者： M.Saleem Khan Zhong Li Ke Yang Dake Xu Chunguang Yang Dan Liu Yassir Lekbach Enze Zhou Phuri Kalnaowakul Institute of Metal Research Chinese Academy of Sciences University of Chinese Academy of Sciences Corrosion and Protection Division Shenyang National Laboratory for Materials Science Northeastern University Laboratory of Microbial Biotechnology Faculty of Science and Technology Sidi Mohamed Ben Abdellah University Kasetsart University

Microbiologically influenced corrosion(MIC) is a big threat to the strength and safety of many metallic materials used in different environments throughout the world. The metabolites and bioactivity of the microorganisms cause severe deterioration on the metals. In this study, MIC of pure titanium(Ti) was studied in the presence of a highly corrosive aerobic marine bacterium pseudomonas aeruginosa. The results obtained from electrochemical test showed that Ti was corrosion resistant in the abiotic culture medium after 14 d, while the increased corrosion current density(i_(corr)) obtained from polarization curves and the decreased charge transfer resistance(R_(ct)) from electrochemical impedance spectroscopy(EIS)indicated the accelerated corrosion of Ti caused by P. aeruginosa biofilm. For further confirmation of the above results, the surface of Ti was investigated using scanning electron microscopy(SEM), confocal laser scanning microscopy(CLSM) and X-ray photoelectron spectroscopy(XPS). According to the XPS results, TiO_2 was formed in both abiotic and biotic conditions, while unstable oxide Ti_2O_3 was detected in the presence of P. aeruginosa, leading to the defects in the passive film and localized corrosion. Pitting corrosion was investigated with the help of CLSM, and the largest pit depth found on Ti surface immersed in P. aeruginosa was 1.2 μm. Ti was not immune to MIC caused by P. aeruginosa.

关键词： Ti Microbiologically influenced corrosion pseudomonas aeruginosa

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H-NS family protein MvaU downregulates phenazine-1-carboxylic acid(PCA)biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium pseudomonas strain PA1201

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Synthetic and Systems Biotechnology 2021年第4期6卷 262-271页

作者： Yun-Ling Fang Ying Cui Lian Zhou Chitti Thawai Tatheer Alam Naqvi Hong-Yan Zhang Ya-Wen He State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental SciencesSJTU-Shanghai Nong Le Joint R&D Center on Biopesticides and BiofertilizersShanghai Jiao Tong University(SJTU)Shanghai200240China Department of Biology Faculty of ScienceKing Mongkut’s Institute of Technology LadkrabangBangkokThailand Department of Biotechnology COMSATS University IslamabadAbbottabad CampusAbbottabadPakistan Shanghai Nong Le Biological Products Company Limited Shanghai201419China

Histone-like nucleoid-structuring(H-NS)proteins are key regulators in gene expression silencing and in nucleoid compaction.The H-NS family member proteins MvaU in pseudomonas aeruginosa are thought to bind the same AT-rich regions of chromosomes and function to coordinate the control of a common set of genes.Here,we explored the molecular mechanism by which MvaU controls PCA biosynthesis in P.aeruginosa PA1201.We present evidence suggesting that MvaU is self-regulated.Deletion of mvaU significantly increased PCA production,and PCA production sharply decreased when mvaU was over-expressed.MvaU transcriptionally repressed phz2 cluster expression and consequently reduced PCA biosynthesis.β-galactosidase assays confirmed that base pairing near the􀀀35 box is required when MvaU regulates PCA production in PA1201.Electrophoretic mobility shift assays(EMSA)and additional point mutation analysis demonstrated that MvaU directly bound to an AT-rich motif within the promoter of the phz2 cluster.Chromatin immunoprecipitation(ChIP)analysis also indicated that MvaU directly bound to the P5 region of the phz2 cluster promoter.MvaU repression of PCA biosynthesis was independent of QscR and OxyR in PA1201 and neither PCA or H2O2 were the environmental signals that induced mvaU expression.These findings detail a new MvaU-dependent regulatory pathway of PCA biosynthesis in PA1201 and provide a foundation to increase PCA fermentation titer by genetic engineering.

关键词： pseudomonas Phenazine-1-carboxylic acid phz cluster MvaU Regulatory network

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pseudomonas sp.J1生物合成纳米硒机制研究

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广西大学学报（自然科学版） 2022年第1期47卷 219-228页

作者：张小萱任国栋刘畅陶文超张笑迎刘欢闵婷梅运军武汉轻工大学化学与环境工程学院湖北武汉430023 武汉轻工大学电气与电子工程学院湖北武汉430023 武汉轻工大学食品科学与工程学院湖北武汉430023

为了探究pseudomonas sp.J1生物合成纳米硒的机制,采用电镜技术、X射线能谱分析法(EDS)及X射线光电子能谱分析法(XPS)对pseudomonas sp.J1合成纳米硒过程及产物进行表征;通过添加谷胱甘肽抑制剂、改变培养基中NO_(3)^(-)、亚硒酸钠浓度... 详细信息

为了探究pseudomonas sp.J1生物合成纳米硒的机制,采用电镜技术、X射线能谱分析法(EDS)及X射线光电子能谱分析法(XPS)对pseudomonas sp.J1合成纳米硒过程及产物进行表征;通过添加谷胱甘肽抑制剂、改变培养基中NO_(3)^(-)、亚硒酸钠浓度以及培养温度等条件研究各因素对生物合成纳米硒的影响。结果表明:pseudomonas sp.J1在胞质内合成纳米硒并向胞外转运;在生物合成纳米硒中谷胱甘肽起到了重要作用;亚硒酸钠浓度越高对细胞毒性越大;NO_(3)^(-)与SeO_(3)^(2-)形成竞争关系,不利于纳米硒的合成;当培养基中亚硒酸钠质量浓度为5 g/L且发酵温度为30℃时,pseudomonas sp.J1转化效率最高,达到(47.73±2.15)% 。

关键词： pseudomonas 纳米硒生物合成

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Effect of nalidixic acid on the morphology and protein expression of pseudomonas aeruginosa

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Asian Pacific Journal of Tropical Medicine 2012年第4期5卷 265-269页

作者： Saif Al Bahry Nallusamy Sivakumar Manal Al-Khambashi Department of Biology College of ScienceP.O.Box:36P.C.123Sultan Qaboos UniversitySultanate of Oman

Objective:To determine the effect of nalidixic acid on the morphology and protein expression of pseudomonas aeruginosa(P.aeruginosa).Methods:Nalidixic acid solution of 1 600μg/mL was prepared.The minimum inhibitory concentration(MIC) for P.aeruginosa was determined with tube dilution test.The effect of nalidixic acid on the morphology of P.aeruginosa was studied using light microscope and scanning electron microscope.Changes in protein profile were studied using SDS-PAGE.Results:The MIC of nalidixic acid was 700μg/mL against P.aeruginosa.The exposure of P.aeruginosa to different concentrations of nalidixic acid resulted in deformation of most of the growing cells.At the concentration of 600μg/mL most of the cells turned into elongated and adhere to each other while some of the cells were bulged.The intensity of protein bands were changed when they exposed to nalidixic acid.Conclusions:The present findings suggest that the morphology and protein expression of P.aeruginosa is greatly affected by nalidixic acid.

关键词： Nalidixic acid pseudomonas aeruginosa Deformed cells Scanning electron microscopy

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Effects of Bacterial-Feeding Nematodes and Glucose on Phenanthrene Removal by pseudomonas putida

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Pedosphere 2017年第1期27卷 165-171页

作者： JING Yongping LIYan LIU Zhaohui ZHANG Yingpeng LIU Ping SUN Ming Jiafa LUO Institute of Agricultural Resources and Environment Shandong Academy of Agricultural Sciences Jinan 250100 (China) Key Laboratory of Agro-Environment in Huang-Huai-Hai Plain Ministry of Agriculture Jinan 250100 (China) Shandong Provincial Key Laboratory of Plant Nutrition and Fertilizer Jinan 250100 (China) Shandong Academy of Agricultural Sciences Jinan 250100 (China) AgResearch Hamilton 3240 (New Zealand)

Bacterial-feeding nematodes can promote the bacterial activity through feeding.Bacterial abundance and their activity affect the degradation of polycyclic aromatic hydrocarbons(PAH) such as phenanthrene.The effects of bacterial-feeding nematodes,bacteria,and their interactions on the degradation of phenanthrene with or without glucose were studied through a microcosm experiment.The results showed that up to 57.0%of phenanthrene in mineral medium contaminated with phenanthrene was degraded in the control with bacteria alone and bacteria with the presence of nematodes and/or glucose increased the degradation of phenanthrene by 25.6%to 36.6%.Although both nematode and bacteria abundance decreased gradually,catechol 2,3-dioxygenase(C230) activity increased during the incubation period.Compared with bacteria alone,the presence of nematodes significantly increased C230 activity as well as the abundance of bacteria;this effect was more pronounced when glucose was present.The results imply that nematodes might promote the removal of phenanthrene from medium by stimulating bacteria and C230 activities.

关键词：食细菌线虫 pseudomonas 葡萄糖菲细菌丰度细菌活动多环芳烃相互作用

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A rapid, simple, and sensitive immunoagglutination assay with silica nanoparticles for serotype identification of pseudomonas aeruginosa

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Journal of Integrative Agriculture 2019年第8期18卷 1936-1943页

作者： DONG Xiu-mei TAO Jing LI Ting-ting ZHANG Ping ZHU Yan TANG Yu SU Rui-hong SHI Dong-fang College of Veterinary Medicine Northeast Agricultural University Harbin 150030 P.R.China Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development College of Veterinary MedicineNortheast Agricultural University Harbin 150030 P.R.China

An agglutination test based on colored silica nanoparticles(colored SiNps) was established to detect serotypes of pseudomonas aeruginosa. Monodisperse colored SiNps were used as agglutination test carriers. The colored SiNps were prepared through reverse microemulsion with reactive dyes, sensitized with 11 kinds of mono-specific antibodies against P. aeruginosa, and denoted as IgG-colored SiNps. Eleven kinds of IgG-colored SiNps were individually mixed with P. aeruginosa on a glass slide.Different serotypes of P. aeruginosa could be identified by agglutination test with evident agglutination. The P. aeruginosa could be detected in a range from 3.6×10^5 to 3.6×10^12 cfu mL^–1. This new agglutination test was confirmed to be a specific,sensitive, fast, easy-to-perform, and cost-efficient tool for the routine diagnosis of P. aeruginosa.

关键词： pseudomonas aeruginosa colored silica nanoparticles agglutination test serotype detection

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Identification and Characterization of Genes Responsible for Drought Tolerance in Rice Mediated by pseudomonas fluorescens

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Rice science 2017年第5期24卷 291-298页

作者： Manjesh SAAKRE Thirthikar Meera BABURAO Abida Puthenpeedikal SALIM Rose Mary FFANCIES Valasala Poothecty ACHUTHAN George THOMAS Sajeevan Radha SIVARAJAN Centre for Plant Biotechnology and Molecular Biology/Department of Genetics and Plant Breeding/Department of Agronomy College of Horticulture Kerala Agricultural University Vellanikkara Thrissur 680656 Kerala India

Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential.Structural and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants respond and adapt to the drought stress.In the present study,differential display technique was employed to study the gene expression of rice plants at the reproductive stage that were subjected to drought stress by withholding water,pseudomonas fluorescens strain(Pf1) treated plants subjected for drought stress by withholding water and control(well-watered).Differentially expressed c DNAs of six genes(COX1,PKDP,b ZIP1,AP2-EREBP,Hsp20 and COC1) were identified,cloned and sequenced.Real-time q PCR analysis showed that all the six genes were upregulated in drought-stressed plants treated with Pf1.This revealed that the remarkable influence of Pf1 colonization leads to drought tolerance at the reproductive stage.These results showed that high levels of gene expression in plants lacking adequate water can be remarkably influenced by Pf1 colonization,which might be a key element for induced systemic tolerance by microbes.

关键词： rice drought tolerance pseudomonas fluorescens differential display reverse transcription polymerase chain reaction quantitative real-time PCR transcript derived fragment

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Selective pseudosolubilization capability of pseudomonas sp. DG17 on n-alkanes and uptake mechanisms analysis

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Frontiers of Environmental Science & Engineering 2013年第4期7卷 539-551页

作者： Fei HUA Hongqi WANG College of Water Sciences Beijing Normal University Beijing 100875 China

Pseudosolubilized ability of pseudomonas sp. DG17 on n-alkanes, role of biosurfactants in n-octadecane uptake and trans-membrane transport mechanism of n- octadecane were studied by analyzing amount of pseudo- solubilized oil components in water phase, and the fraction of radiolabeled ~4C n-octadecane in the broth and cell pellet. GC-MS results showed that pseudosolubilized oil components were mainly C12 to C28 of n-alkanes. In n- octadecane broth, pseudosolubilized n-octadecane could be accumulated as long as faster than mineralization maximum concentration of cane achieved to 45.37 mg. L pseudosolubilized rate was rate of substrate, and the pseudosolubilized n-octade- 1. All of these results showed that pseudomonas sp. DG17 mainly utilized alkanes by directly contacting with pseudosolubilized small oil droplets in the water phase. Analysis of 14C amount in cell pellet revealed that an energy-dependent system mainly controlled the trans-membrane transport of n- octadecane.

关键词： pseudomonas alkane uptake pseudosolubi-lization trans-membrane transport

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Discovery of synergistic activity of fluoroquinolones in combination with antimicrobial peptides against clinical polymyxin-resistant pseudomonas aeruginosa DK2

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Chinese Chemical Letters 2020年第2期31卷 413-417页

作者： Xinyu Zheng Qiao Cao Qin Cao Fei Mao Xiaokang Li Jin Zhu Lefu Lan Jian Li State Key Laboratory of Bioreactor Engineering Shanghai Key Laboratory of New Drug DesignSchool of PharmacyEast China University of Science and TechnologyShanghai 200237China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Polymyxin B(PB),as the last-line of defense against multidrug-resistant Gram-negative bacteria,has caused resistance to P.aeruginosa recently.Fortunately,synergistic treatment could preserve the last class of antibiotics and reduce the emergency of drug resistance.Here,we performed a screen of 970 approved drugs synergized with PB against the P.aeruginosa DK2,which is severely resistant to PB,MIC=512μg/mL.Encouragingly,we found fluoroquinolones could synergy with PB and achieved an obvious reduction in MIC of PB below the clinical susceptible breakpoint(2 μg/mL).Especially,gemifloxacin achieved the highest synergistic effect with PB,leading to a 4096-fold MIC reduction(reduced from512 μg/mL to 0.125 μg/mL).Furthermore,synergistic effect was also observed in the combination of gemifloxacin and colistin.Finally,outer membrane permeabilization assay showed that gemifloxacin could increase the permeability of bacterial cell membranes for P.aeruginosa which partly explained the synergy mechanism.These results indicate that fluoroquinolones represent attractive synergists to address the emerging threat of polymyxin-resistant infections.

关键词： Polymyxin B pseudomonas aeruginosa Fluoroquinolones Synergy Cystic fibrosis Antimicrobial peptides

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Colistin Resistance Profiles, Molecular Investigation of mcr-1 and mcr-2 Plasmid Genes and Investigation of Carbapenemase Production in pseudomonas and Acinetobacter Strains

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Open Journal of Medical Microbiology 2023年第4期13卷 276-291页

作者： Jean Fabrice Yala Hilaire Kenguele Moundounga Rolande Mabika Mabika Franck Mounioko Ornella Zong Minko Sougouna Henda Rokyatou Bikieya Massima Alain Souza Laboratoire de Biologie Moléculaire et Cellulaire Equipe de Microbiologie (LABMC) Unité de Recherche Agrobiologie Université des Sciences et Techniques de Masuku (USTM) Franceville Gabon Laboratoire de Bactériologie Unité de Recherche d’Analyses Médicales (URAM) Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF) Franceville Gabon Unité d’Ecologie des Systèmes Vectoriels (ESV) Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF) France-ville Gabon Laboratoire de Physiologie animale et Pharmacologie Unité de recherche Agrobiologie Université des Sciences et Techniques de Ma-suku (USTM) Franceville Gabon

Background and Purpose: The reintroduction of colistin as a last resort treatment against multi-resistant Gram-negative bacilli, is currently challenged by the emergence of colistin-resistant bacteria. The aim of this study was to assess the susceptibility of pseudomonas and Acinetobacter strains to colistin, to identify carbapenemase production, and to investigate the plasmid genes involved in colistin resistance and carbapenemase production. Methodology: In order to establish the susceptibility profiles of 17 strains of pseudomonas and Acinetobacter to colistin, their Minimum Inhibitory Concentrations (MICs) were determined using the liquid microdilution method. The possible production of carbapenemases was investigated with the modified Carbapenem Inactivation Method (mCIM). The search for genes encoding carbapenemases (blaOXA, blaIMP, blaCarba) and those responsible for plasmid resistance to colistin (mcr-1 and mcr-2) was performed by conventional PCR. Results and Conclusion: Ninety-four percent (94%) (16/17) of the strains were resistant to colistin. Intraspecies distribution was 50% (8/16), 31% (5/16), 13% (2/16) and 6% (1/16) for Acinetobacter baumannii, pseudomonas aeruginosa, pseudomonas luteola, and pseudomonas fluorescens, respectively. Twenty-nine percent (29%) (6/17) of the strains produced carbapenemases. No mcr-1 and mcr-2 plasmid genes were detected. On the other hand, 17.6% (3/17) of the strains possessed the carbapenemase genes distributed as follows: Carba type (60%), OXA type (40%) and IMP type (0%). The results of this study highlight a high resistance to colistin in strains belonging to the genera Acinetobacter and pseudomonas, and some of these strains produce carbapenemases.

关键词： pseudomonas Acinetobacter Carbapenemase Colistin Suceptibility

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