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Study on Culturing prunus salicina *** Embryos

Agricultural Science & Technology 2010年第4期11卷 80-82,166页

作者：余小玲何业华韩景忠谢志亮彭兵华南农业大学园艺学院广东广州510642 广东外语外贸大学南国商学院广东广州510420

[Objective]The aim was to study the effective factors on culturing prunus salicina *** embryos in vitro.[Method]Different age,culture medium and GA3 which affected culturing of prumssalicina embryos were discussed.[Result]The result showed that on the part of 26-41 d,after the blooming period,the embryos remained tiny and retained endosperms and showed no signs of change after having cultured for three *** the part of 48 d,after the blooming period,the endosperms had disappeared,the embryos kept growing until they filled the seed cavity;when they were planted on the MS culture medium,their survival rate reached 77%,in its first generation,the response of embryos was *** the part of 65 d,after the blooming period,4.5% of their embryos grew into shoots on the MS culture medium;with the age of embryos growing,the survival rate of shoots increased until it reached 26% when the fruits went into ripeness;the embryos produced calli in their first generation of *** the part of 65-83 d,after the blooming period,the embryos produced calli through more than 2-3 *** the part of 88 d,after the blooming period,the survival rate of shoots on the WPM culturing base doubled compared with that on the MS culturing base;on the same culture medium,the embryos were inhibited from growing into shoots when BA,KT or 2,4-D was added on to the culture *** survival rate of shoots was increased remarkably when the seeds were treated in 1 000 mg/L GA3.[Conclusion] This study provided experimental basis for the establishment of prunus salicina ***,embryos rescue techniques and cross breading.

关键词： prunus salicina cv.zaoshi Embryos Culture in vitro Age of embryo

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