检索结果-南通市图书馆

Evaluation of Isoflavones as Allelochemicals with Strong Allelopathic Activities of Kudzu Using protoplast Co-culture Method with Digital Image Analysis

引用

American Journal of Plant Sciences 2021年第3期12卷 376-393页

作者： Kentaro Kobayashi Hamako Sasamoto Yutaka Sasamoto Akifumi Sugiyama Yoshiharu Fujii Department of International Environmental and Agricultural Science Faculty of Agriculture Tokyo University of Agriculture and Technology Fuchu Japan Research Institute for Sustainable Humanosphere (RISH) Kyoto University Uji Japan

The inhibitory allelopathic activity of Pueraria montana (Kudzu), and activities of two putative allelochemical isoflavones, puerarin and daidzein, were evaluated using the protoplast co-culture method with digital image analysis using lettuce as a recipient (DIA-PP method). Cotyledon protoplasts of Kudzu were isolated using Cellulase R10 and Driselase 20 in 0.6 M mannitol solution. Optimal hormonal condition and density for growth of Kudzu protoplasts were surveyed. Medium for co-culture of Kudzu or isoflavones with lettuce protoplasts was 50 μl liquid MS basal medium containing 1 μM 2,4-dichlorophenoxyacetic acid, 0.1 μM benzyladenine, 3% sucrose, and 0.4 M or 0.6 M mannitol. Protoplast division of lettuce was strongly inhibited by Kudzu at a low density (104/ml). Slightly less inhibition by Kudzu on cell wall formation and yellow pigment accumulation stages of lettuce growth was also observed. Puerarin did not inhibit the growth of lettuce protoplasts at three growth stages but slightly stimulated growth at high concentrations. By contrast, daidzein, aglycon of puerarin, inhibited growth at three stages of lettuce protoplast growth and strongly inhibited cell division at 100 μM. Daidzein might be one cause of the strong inhibitory allelopathic activity of Kudzu. Grade of inhibitory activities was compared with that of other allelopathic plants including an invader plant and their allelochemicals studied using the DIA-PP method.

关键词： Allelopathy Bioassay Daidzein Isoflavone protoplast culture Puerarin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research on protoplast preparation and culture in Gladiolus hybridus Hort

引用

Journal of Northeast Agricultural University(English Edition) 2007年第1期14卷 5-8页

作者： ZHANG Ying GONG Sufang WANG Jingang CHE Daidi College of Horticulture Northeast Agricultural University Harbin 150030 China

protoplasts prepared from the aseptic leaves of Rose Supreme in Gladiolus hybridus Hort. were cultured. The yield of protoplasts with vigour was the highest under the following conditions： 1.5% cellulase Onzuka RS, 0.5% pectinase Y-23, 0.6 mol· L^-1 mannitol, pH 5.8, digestion time 2 h, and temperature 27℃. After isolation and purification, protoplasts were cultured in solid and liquid combined medium, which included KM8P, 0.6 mol· L^-1 mannitol, 1.0 mg· L^-1 2, 4-D, 0.2 mg· L^-1 NAA and 0.2 mg· L^-1 KT. The division of the protoplasts first emerged in medium after cultured 4 days, and emerged only a few times.

关键词： Gladiolus hybridus aseptic leaves protoplast culture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Plant regeneration from protoplasts of hydroxyprolineresistant cell line in Onobrychis viciaefolia

引用

Cell Research 1995年第2期5卷 187-194页

作者： XU ZIQIN JINGFEN JIA(Cell Blology Laboratory, Lanzhou University, Lanzhou 730000, China.) Cell Biology Laboratory Lanzhou University Lanzhou China

An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onoblychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2, 4-dichlorophenoxy-acetic acid (2,4-D), 0.5 mg/L kinetin (KT) and 0.2 mg/L naphthalene acetic acid (NAA), the division frequency of protoplastderived cells reached uP to over 60 %, and microcalli were obtained in 5-6 wk. Upon transferring them on agar solidified MS medium plus 2 mg/L indole-3-acetic acid (IAA), shoots were induced. After cultivating them on MS medium with or without IAA, roots were *** number of all protoplast-regenerated plants examined were normal (2n=28). The protoplast-derived calli and plants grew vigorously on the medium containing 10 mmol/L HYP.

关键词： Sainfoin (Onobrychis Viciaefolia) hydroxyproline resistant cell line protoplast culture plant regeneration

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Plant regeneration from cultured protoplasts of a glutinous rice

引用

Cell Research 1990年第1期1卷 17-21页

作者： Wang Guangyuan ,Hsia Chenau(Xia Zhenao), Chi Jinfen and Gao XiaoyanShanghai Institute of Plant Physiology, Academia Sinica Shanghai Institute of Plant Physiology Academia Sinica

Young embryos of rice （Oryza sativa L. subsp, japonicavar. Guo-xiang No. 1） were cultured on MS agar medium（2,4-D 2 mg/l）. Calli were formed and subcultured on N6 agar medium （2,4-D 2 mg/l ）. After selection, the small, grainy and pale yellowish cell clusters with dense cytoplasm were used in protoplast preparation. Isolated protoplasts were cultured in N6 medium （2,4-D 1 mg/l, 6-BA 0.2 mg/l）1 with agarose block culture method. The protoplasts grew, divided and formed calli. After inducing differentiation, the regenerated mature plants were obtained.

关键词： protoplast culture Oryza saliva glutinous va-riety plant regeneration.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：