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Basal repertoire of toxin-related transcripts in the zoanthid protopalythoa variabilis(Cnidaria,Anthozoa)

中国药理学与毒理学杂志 2015年第S1期29卷 104-104页

作者： ChenHUANG Jean-EtienneRLMORLIGHEM HefengZHOU EricaPatríciaLIMA PaulaBragaGOMES CarlosDanielPEREZ InchioLOU SimonMingYuenLEE GRadis-BAPTISTA State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences University of Macao Northeast Biotechnology Network Post-graduation program in Biotechnology Federal University of Ceara Laboratory of Biochemistry and Biotechnology Institute for Marine Sciences Federal University of Ceara Centro Acadêmico de Vitoria Universidade Federal de Pernambuco Departamento de Biologia Universidade Federal Rural de Pernambuco Faculty of Science and Technology Department of Civil and Environmental Engineering University of Macao Macao China

OBJECTIVE To analyze the whole transcriptome of zoanthid protopalythoa variabilis(***),a cnidarian,and discover the potential toxic substances in *** The *** RNA deep sequencing was performed using the HiSeq 2500 automatic sequencing *** the unigenes generated from the assembly process were functionally annotated based on the similarity with *** multiple alignments of translated toxin-related sequences were performed with Clustalw2,and amino acid identity and similarity highlighted by using BoxShade *** different methods including ITASSER,PEP-FOLD and MODELLER were applied to predict tri-dimensional models of toxin-related polypeptides from translated transcript sequences of *** toxicity of one of the putative toxins,namely ShK/Aurelin-like peptide,was evaluated using zebrafish *** A total of 67,549,914 pairs of quality-filtered,90-base-pair Illumina reads from an mRNA sample were *** de novo assemblies yielded 276,526 *** sequence comparison of 130,121 unigenes with entries in Toxin database showed that 1542 unigenes were potential peptide toxins at which 11 unigenes were related to Stichodactyla toxin(ShK)domain(Pfam ID:PF01549).ShK is a 35 residues peptide sequence that was firstly discovered from the sea anemone Stichodactyla ***,we found out one ShK-like peptide that processed a relatively higher sequence similarity with known ShK(Uniprot ID:P29186)of Bunodosoma granuliferum(red warty sea anemone).The protopalythoa Shk-like peptide was submitted to Probis server to detect probable binding site and found to match with a protein AURELIN(PDB id:2lg4,UniProt id:Q0MWV8)which possesses structural homology with previously identified antimicrobial peptides and K+-channel-blocking *** results showed that the ShK/Aurelin-like peptide was lethal to zebrafish embryos at concentrations above 30-μmol·L1,and could induce zebrafish locomotor deficit at 10μmol

关键词： zoanthidea transcriptome peptide toxin protopalyth

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