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Optimization of the Ultrasonic-assisted Extraction Process for protocatechuic acid from Emilia sonchifolia DC by Response Surface Methodology

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Agricultural Biotechnology 2020年第3期9卷 58-66页

作者： Wen CHEN Xiangjun WANG Lingyan LI Zhewen ZHAI Xiaoling HUANG Wubing RAO Chenghan ZOU Tengtong LI College of science Hainan Tropic Ocean UniversitySanya 572022China College of Fisheries and life Hainan Tropic Ocean UniversitySanya 572022China College of Food Science Southwest UniversityChongqing 400715China

[Objectives] This study was conducted to optimize the extraction process of protocatechuic acid from Emilia sonchifolia DC. [Methods] The optimal extraction conditions were determined by single factor,response surface analysis and variance analysis,and the content of protocatechuic acid was determined by HPLC. [Results] The protocatechuic acid standard curve equation was: y = 1 435 x + 8 403,R^2= 0. 999 8,indicating a good linear relationship. The optimal extraction conditions were as follows: a temperature at 80 ℃,an extraction time of 1 h,a material-to-liquid ratio at 1:10 and an ultrasonic power of 600 W,and the content of protocatechuic acid extracted was 1. 93 mg/g. The method showed a RSD of 0. 41%,less than 2%,and the detection limit was 0. 0000047261 g/*** experimental sample X1 was the low-level 0. 1 mg/ml standard solution,which showed recovery of protocatechuic acid between 100.8% and 105.2%,with a RSD of 0. 013%;and the sample X2 was the high-level 1. 0 mg/ml standard solution,which exhibited recovery between 100. 6% and 102. 2%,with a RSD of 0.076%. Thus,the recovery was high,and the requirements of the performance index were met. [Conclusions] The detection method is stable and reliable and can produce satisfactory results.

关键词： Emilia sonchifolia DC protocatechuic acid HPLC Response surface optimization Variance analysis

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Simultaneous Determination of eight constituents in Qin-Lian-Si-Wu Decoction by High-Performance Liquid Chromatography-DAD

Simultaneous Determination of eight constituents in Qin-Lian...

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中华中医药学会中药分析分会第三届学术交流会

作者：刘春梅 Jiangsu Key laboratory for TCM formulae Research Nanjing University of Chinese Medicine

A high-performance liquid chromatography-DAD method was applied to the determination of vanillic acid,caffeic acid,ferulic acid,protocatechuic acid,paeoniflorin, baicalin and scutellarin in Qin-Lian-Si-Wu decoction and other 5 crude *** eight compounds were analysed simultaneously with a Diamonsil C-18 column by gradient elution using 0.5%(v/v) acetic acid-acetonitrile as the mobile *** follow rate was 0.8mL/min, the temperate was 30℃,and detection was set at 230,260,280,310,323 *** linearity (r≥0.9995) of the 8 compounds was good in a relatively wide concertration rage,and the average recovery was 96.1～101.3%.The result indicated that the content of these eight compounds changed after decocting *** the compounds decreased significantly.

关键词： HPLC Qin-Lian-Si-Wu Decoction vanillic acid caffeic acid ferulic acid protocatechuic acid paeoniflorin baicalin scutellarin Chuanxiongzine

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