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SF/HGF-c-met autocrine and paracrine promote metastasis of hepatocellular carcinoma

World Journal of Gastroenterology 2001年第6期7卷 816-820页

作者： Qian Xie Kang-Da Liu Mei-Yu Hu Kang Zhou Experimental Research center of Zhongshan Hospital,Fudan University,Shanghai,200032,china Experimental Research center of Zhongshan Hospital Fudan University Shanghai 200032 China.

AIM: To explore the role of SF/HGF-met autocrine and paracrine in metastasis of hepatocellular carcinoma (Hcc). metHODS: SF/HGF and c-met transcription and protein expression in Hcc were examined by RT-PcR and Western Blot in 4 Hcc cell lines, including HepG2, Hep3B, SMMc7721 and MHcc-1, the last cell line had a higher potential of metastasis. sf/hgf cDNA was transfected by the method of Lipofectin into SMMc7721. SF/HGF and c-met antibody were used to stimulate and block SF/HGF-c-met signal transduction. cell morphology, mobility, and proliferation were respectively compared by microscopic observation, wound healing assay and cell growth curve. RESULTS: Hcc malignancy appeared to be relative to its met-SF/HGF expression. In MHcc-1, c-met expression was much stronger than that in other cell lines with lower potential of metastasis and only SF/HGF autocrine existed in MHcc-1. After sf/hgf cDNA transfection or conditioned medium of MHcc-1 stimulation, SMMc7721 changed into elongated morphology, and the abilities of proliferation (P creased. Such bio-activity could be blocked by c-met antibody (P cONcLUSION: The system of SF/HGF-c-met autocrine and paracrine played an important role in development and metastasis potential of Hcc. Inhibition of SF/HGF-c-met signal transduction system may reduce the growth and metastasis of Hcc.

关键词： Autocrine communication carcinoma, Hepatocellular Hepatocyte Growth Factor Humans Liver Neoplasms Paracrine communication proto-oncogene proteins c-met Research Support, Non-U.S. Gov't Tumor cells, cultured

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