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Differential subcellular distribution renders HAI-2 a less effective protease inhibitor than HAI-1 in the control of extracellular matriptase proteolytic activity

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Genes & Diseases 2022年第4期9卷 1049-1061页

作者： Yi-Lin Chiu Yi-Ying Wu Robert B.Barndt Yu-Wen Lin Hou-Ping Sytwo Amy Cheng Kacy Yang Khee-Siang Chan Jehng-Kang Wang Michael D.Johnson Chen-Yong Lin Lombardi Comprehensive Cancer Center Department of Oncology Georgetown UniversityWashingtonDC 20057USA Department of Biochemistry National Defense Medical CenterTapei 114Chinese Taipei Division of Hematology/Oncology Department of Internal MedicineTri-Service General HospitalNational Defense Medical CenterTapei 114Chinese Taipei School of Medicine National Defense Medical CenterTapei 114Chinese Taipei Department of Intensive Care Medicine Chi Mei Medical CenterTainan 710Chinese Taipei

The integral membrane,Kunitz-type serine protease inhibitors HAI-1 and HAI-2,can suppress the proteolytic activity of the type 2 transmembrane serine protease matriptase with high specificity and *** levels of extracellular matriptase proteolytic activity have,however,been observed in some neoplastic B-cells with high levels of endogenous HAI-2,indicating that HAI-2 may be an ineffective matriptase inhibitor at the cellular *** different effectiveness of the HAIs in the control of extracellular matriptase proteolytic activity is examined *** inducing matriptase zymogen activation in the HAI Teton Daudi Burkitt lymphoma cells,which naturally express matriptase with very low levels of HAI-2 and no HAI-1,nascent active matriptase was rapidly inhibited or shed as an enzymatically active *** increasing HAI-1 expression,cellular matriptase-HAI-1 complex increased,and extracellular active matriptase decreased *** HAI-2 expression,however,resulted in cellular matriptase-HAI-2 complex levels reaching a plateau,while extracellular active matriptase remained *** contrast to this differential effect,both HAI-1 and HAI-2,even at very low levels,were shown to promote the expression and cell-surface translocation of endogenous *** difference in the suppression of extracellular active matriptase by the two closely related serine protease inhibitors could result from the primarily cell surface expression of HAI-1 compared to the mainly intracellular localization of *** HAIs,therefore,resemble one another with respect to promoting matriptase expression and surface translocation but differ in their effectiveness in the control of extracellular matriptase enzymatic activity.

关键词： HAI-1 HAI-2 Matriptase Neoplastic B-cells proteolytic activity

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Phenotypic and Genetic Characterization of Bacillus Species Exhibiting Strong proteolytic activity Isolated from Terasi,An Indonesian Fermented Seafood Product

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Journal of Northeast Agricultural University(English Edition) 2015年第4期22卷 15-22页

作者： Ekachai Chukeatirote Novi Arfarita Piyanuch Niamsup Anittaya Kanghae School of Science Mae Fah Luang University Faculty of Agriculture Brawijaya University Faculty of Science Maejo University

In this study, two bacilli strains namely S2-3 and S4-5, isolated from Terasi, a traditional fermented seafood product of Indonesia, were studied in terms of their phenotypic and genotypic properties. Both strains are of great interests due to their high proteolytic activity. Initially, they were subjected to morphological determination and a series of biochemical tests. These bacteria were Gram-positive, endospore-forming bacilli. Based on 16S rRNA gene sequence analysis, the identities of the strains S2-3 and S4-5 were confirmed as Bacillus thuringiensis and B. subtilis, respectively. Additionally, the two strains were also evaluated for their antibiogram profiles. It was found that they were susceptible to chloramphenicol, erythromycin, kanamycin, tetracycline and vancomycin and resistant to ampicillin and intermediately susceptible to bacitracin.

关键词： Bacillus fermented seafood proteolytic activity Terasi

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Heterogenous Expression of Synechocystis sp. PCC 6803 Deg Proteases and Their Possible Roles on Phycobiliprotein Degradation in vitro

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Journal of Wuhan University of Technology(Materials Science) 2011年第6期26卷 1049-1058页

作者：刁红丽周明 School of Life Science and Technology Huazhong University of Science and Technology School of Resource and Environmental Engineering Wuhan University of Technology

The Synechocystis sp. PCC 6803 genome harbours a Deg gene family consisting of three members, htrA （degP, slr1204）, hhoA （degQ, sll1679） and hhoB （degS, sll1427）. This work provided biochemical characterization of HhoA, HtrA and HhoB from Synechocystis sp. PCC 6803. Firstly mature HhoA, HhoB and HtrA from Synechocystis sp. PCC 6803 were cloned and expressed as soluble recombinant his-tagged fusion protein in Escherichia coli. Then the proteolytic activity of HhoA, HhoB and HtrA was tested using casein, bovine serum albumin, five recombinant chromoproteins and cyanobacterial phycocyanin as substrates in vitro. The experimental results showed that HhoA and HtrA had proteolytic activity on casein, five recombinant chromoproteins and cyanobacterial phycocyanin. No proteolytic activity of HhoB was found using all substrates in vitro, indicating functional difference among Deg proteases from Synechocystis sp. PCC 6803. Therefore, the results indicated the biochemical properties of HhoA and HtrA on hydrolysis of proteins and phycobiliproteins in vitro, which implicated that they were proteases possibly involved in phycobiliprotein degradation in vivo.

关键词： Deg protease proteolytic activity phycobiliprotein recombinant chromoprotein Phycobilisome(PBS) degradation

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Effect of proteolytic starter culture isolated from Chinese Dong fermented pork(Nanx Wudl)on microbiological,biochemical and organoleptic attributes in dry fermented sausages

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Food Science and Human Wellness 2021年第1期10卷 13-22页

作者： Xi Chen Ruifang Mi Biao Qi Suyue Xiong Jiapeng Li Chao Qu Xiaoling Qiao Wenhua Chen Shouwei Wang China Meat Research Center Beijing 100068China Beijing Key Laboratory of Meat Processing Technology Beijing 100068China

The effect of a proteolytic starter culture isolated from Nanx Wudl,on microbiological,biochemical and organoleptic attributes of dry fermented sausages was investigated during *** on preliminary screening,the combination of Staphylococcus xylosus SX16 and Lactobacillus plantarum CMRC6,showing excellent proteolytic activity in vitro,was selected as the multi-strain starter(starter LS).For comparison,the single-strain starter culture of *** CMRC6(starter LB)and non-inoculated control were also *** fermentation,lactic acid bacteria and staphylococci dominated the microbiota and suppressed the Enterobacteriaceae growth in LS-inoculated *** addition of LS starter accelerated acidification and proteolysis during ripening,improving the contents of total free amino acids and several essential free amino acids(Phe,Ile and Leu).Volatile compounds analysis revealed that LS-fermented sausage showed the highest abundance of 3-methyl-1-butanol,probably due to the inoculated *** inoculation of LS starter improved the sensory properties of sausages,especially the flavor ***,*** SX16 and *** CMRC6 are promising candidates for inclusion as multi-strain starters in the manufacture of gourmet fermented dry sausage.

关键词： Staphylococcus xylosus proteolytic activity Lactobacillus plantarum Nanx Wudl fermented sausage

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The combined effect of ultrasound treatment and leek (Allium ampeloprasum) extract on the quality properties of beef

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Food Bioscience 2022年第3期47卷 1060-1069页

作者： Ali Mehrabani Ashkan Jebelli Javan Mohammad Ali Hesarinejad Ali Mahdavi Mahnoosh Parsaeimehr Department of Food Hygiene Faculty of Veterinary MedicineSemnan UniversitySemnanIran Department of Food Processing Research Institute of Food Science and Technology (RIFST)MashhadIran

One of the most important physicochemical properties of meat is its *** impact of Leek (Allium ampeloprasum ) extract and ultrasound on meat quality and molecular weight distribution of Longissimus lumborum muscles was *** have been treated with ultrasound (100 and 300 W during 10,20,and 30 min) and Leek-derived exogenous proteases (16.9 and 33.8U/g) simultaneously and *** combined effect of enzyme and ultrasound in 10 and 20 min increased the pH *** was also increased the proteolytic activity of *** meat quality,the filtering residues,cooking loss,shear force,and hardness were reduced;water-binding capacity,emulsion capacity,and emulsion stability were *** electrophoretic pattern of myofibrillar proteins showed the muscle fibers were severely degraded in the combined *** can be concluded that this process could be used as an alternative to the chemical tenderizing agents in the meat industry.

关键词： Leek extract proteolytic activity Quality properties Tenderization Ultrasound-assisted enzyme treatment

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Partial Purification and Characterization of Protease from Abrus precatorius Linn. (Fabaceae) from Cameroon

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Advances in Enzyme Research 2016年第2期4卷 35-43页

作者： Mezajoug Kenfack Laurette Blandine Ngangoum Eric Serge Tchiégang Clergé Bioprocess Laboratory Unit of Food Sciences and nutrition University Institute of Technology University of Ngaoundere Ngaoundere Cameroon

Crude enzyme extracts were prepared from leaves and stems of Linn. (Fabaceae) from Cameroon under optimized conditions. proteolytic enzymes were precipitated with ammonium sulfate at 35% (w/v) saturation and assayed for enzyme activity. The effects of temperature, pH, incubation time and substrate specificity were studied. SDS-PAGE was used to determine molecular weight of precipitated protease. Results indicated that proteolytic activity of crude extract was 35.20 U/ml compared to 51.03 U/ml of partial purified extract. The optimum enzyme activity was found to be at 40°C, while 50% of activity was maintained at 60°C after 60 min incubation. Partial purified crude extract exhibited two optimum pH (2.75 and 9.0). The highest enzyme activity towards Bovine Serum Albumine (25.9 U/ml) was noted. SDS-PAGE gels exhibited molecular weight between 40 - 60 KDa. This result confirms that partial purified extract of A. precatorius contains proteases and could be a promising source for proteolytic enzyme extraction.

关键词： A. precatorius Cameroon Proteases Partial Purified Extract proteolytic activity

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