检索结果-南通市图书馆

Plastid KEA-type cation/H+ antiporters are required for vacuolar protein trafficking in Arabidopsis

植物学报:英文版 2023年第9期65卷 2157-2174页

作者： Xiao Zhang Lu Wang Ting Pan Xuexia Wu Jinbo Shen Liwen Jiang Hiromi Tajima Eduardo Blumwald Quan-Sheng Qiu MOE Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 73000China Academy of Plateau Science and Sustainability School of Life SciencesQinghai Normal UniversityXining 810000China College of Coastal Agricultural Sciences Guangdong Ocean UniversityZhanjiang 524088China State Key Laboratory of Herbage Improvement and Grassland Agro‐ecosystems Lanzhou UniversityLanzhou 730000China State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou 311300China School of Life Sciences Centre for Cell&Developmental Biology and State Key Laboratory of AgrobiotechnologyThe Chinese University of Hong KongHong KongChina Department of Plant Sciences University of CaliforniaDavisCA 95616USA

Arabidopsis plastid antiporters KEA1 and KEA2are critical for plastid development, photosynthetic efficiency, and plant ***, we show that KEA1 and KEA2 are involved in vacuolar protein trafficking. Genetic analyses found that the kea1 kea2 mutants had short siliques, small seeds, and short seedlings. Molecular and biochemical assays showed that seed storage proteins were missorted out of the cell and the precursor proteins were accumulated in kea1 kea2. protein storage vacuoles(PSVs) were smaller in kea1 kea2. Further analyses showed that endosomal trafficking in kea1 kea2 was compromised. Vacuolar sorting receptor 1(VSR1) subcellular localizations, VSR–cargo interactions, and p24 distribution on the endoplasmic reticulum(ER) and Golgi apparatus were affected in kea1 kea2. Moreover, plastid stromule growth was reduced and plastid association with the endomembrane compartments was disrupted in kea1 kea2. Stromule growth was regulated by the cellular pH and K+homeostasis maintained by KEA1 and KEA2. The organellar pH along the trafficking pathway was altered in kea1 kea2. Overall, KEA1 and KEA2 regulate vacuolar trafficking by controlling the function of plastid stromules via adjusting pH and K+homeostasis.

关键词： Arabidopsis K^(+)homeostasis plastid antiporters plastid stromule pH protein trafficking

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Control of Directional Macromolecular trafficking Across Specific Cellular Boundaries:A Key to Integrative Plant Biology

引用

Journal of Integrative Plant Biology 2007年第8期49卷 1227-1234页

作者： BiaoDing AsukaItaya Department of Plant Cellular and Molecular Biology and Plant Biotechnology Center Ohio State University ColumbusOhio 43210USA The OSU RNA GroupOhio State UniversityColumbusOhio 43210USA MolecularCellular and Developmental Biology ProgramOhio State UniversityColumbusOhio 43210USA

There is now solid evidence that cell-to-cell trafficking of certain proteins and RNAs plays a critical role in trans-cellular regulation of gene expression to coordinate cellular differentiation and development. Such trafficking also is critical for viral infection and plant defense. The mechanisms of trafficking remain poorly understood. Although some proteins may move between cells by diffusion, many proteins and RNAs move in a highly regulated fashion. Regulation is likely achieved through interactions between distinct protein or RNA motifs and cellular factors. Some motifs and factors have been identified. One of the major focuses for future studies is to identify all motifs and their cognate factors and further elucidate their roles in trafficking between specific cells. With increasing information from such studies, we should be able to develop an understanding of the mechanisms that regulate trafficking of various proteins and RNAs across all and specific cellular boundaries. On the basis of such mechanistic knowledge, we can further investigate how the trafficking machinery has evolved to regulate developmental and physiological processes in a plant, how pathogens have co-evolved to use this machinery for systemic spread in a plant, and how plants use this machinery for counterdefense.

关键词： CAPRICE GLABRA3 KNOTTED-I phloem plasmodesmata protein trafficking RNA trafficking SHORT-ROOT viral movementprotein viroid

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SARS-CoV-2 spike host cell surface exposure promoted by a COPI sorting inhibitor

引用

药学学报:英文版 2023年第7期13卷 3043-3053页

作者： Yiqun Li Mingrui Yang Yanan Nan Jiaming Wang Sanjiao Wang Dongxiao Cui Jiajian Guo Pengfei He Wenxin Dai Shuqi Zhou Yue Zhang Wenfu Ma School of Life Science Beijing University of Chinese MedicineBeijing 102488China

Via an insufficient coat protein complex I(COPI)retrieval signal,the majority of SARSCo V-2 spike(S)is resident in host early secretory organelles and a tiny amount is leaked out in cell *** surface-exposed S can be recognized by B cell receptor(BCR)or anti-S therapeutic monoclonal antibodies(m Abs)that is the trigger step for B cell activation after S m RNA vaccination or infected cell clearance by S m ***,a drug strategy to promote S host surface exposure is ***,we first combined structural and biochemical analysis to characterize S COPI sorting signals.A potent S COPI sorting inhibitor was then invented,evidently capable of promoting S surface exposure and facilitating infected cell clearance by S antibody-dependent cellular cytotoxicity(ADCC).Importantly,with the inhibitor as a probe,we revealed Omicron BA.1 S is less cell surface exposed than prototypes because of a constellation of S folding mutations,possibly corresponding to its ER chaperone *** findings not only suggest COPI is a druggable target against COVID-19,but also highlight SARS-Co V-2 evolution mechanism driven by S folding and trafficking mutations.

关键词： COPI inhibitor SARS-CoV-2 spike Spike sorting motifs protein folding protein trafficking Anti-COVID-19 Omicron variants and drug discovery

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Salicylic Acid Signaling Controls the Maturation and Localization of the Arabidopsis Defense protein ACCELERATED CELL DEATH6

引用

Molecular Plant 2014年第8期7卷 1365-1383页

作者： Zhongqin Zhang Jay Shrestha Chika Tateda Jean T. Greenberg Department of Molecular Genetics and Cell Biology University of Chicago 929 East 57 Street GCIS W524 Chicago IL 60637 USA

ACCELERATED CELL DEATH6 （ACD6） is a multipass membrane protein with an ankyrin domain that acts in a positive feedback loop with the defense signal salicylic acid （SA）. This study implemented biochemical approaches to infer changes in ACD6 complexes and localization. In addition to forming endoplasmic reticulum （ER）- and plasma membrane （PM）-Iocalized complexes, ACD6 forms soluble complexes, where it is bound to cytosolic HSP70, ubiquitinated, and degraded via the proteasome. Thus, ACD6 constitutively undergoes ER-associated degradation. During SA signaling, the soluble ACD6 pool decreases, whereas the PM pool increases. Similarly, ACD6-1, an activated version of ACD6 that induces SA, is present at low levels in the soluble fraction and high levels in the PM. However, ACD6 variants with amino acid substitutions in the ankyrin domain form aberrant, inactive complexes, are induced by a SA agonist, but show no PM localization. SA signaling also increases the PM pools of FLAGELLIN SENSING2 （FLS2） and BRI1-ASSOClATED RECEPTOR KINASE 1 （BAK1）. FLS2 forms complexes ACD6; both FLS2 and BAK1 require ACD6 for maximal accumulation at the PM in response to SA signaling. A plausible scenario is that SA increases the efficiency of productive folding and/or complex formation in the ER, such that ACD6, together with FLS2 and BAK1, reaches the cell surface to more effectively promote immune responses.

关键词： ACD6 protein trafficking protein quality control salicylic acid.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana

引用

Molecular Plant 2015年第8期8卷 1175-1187页

作者： Marta Zwiewka Tomasz Nodzynski Stephanie Robert Steffen Vanneste Jiri Friml Mendel Centre for Plant Genomics and Proteomics Central European Institute of Technology (CEITEC) Masaryk University Kamenice 5 CZ-625 00 BrnoCzech Republic Department of Plant Systems Biology VlB and Department of Plant Biotechnology and Bioinformatics Ghent University Technologiepark 927 9052 Gent Belgium Institute of Science and Technology (IST) Austria Am Campus 1 3400 Klosterneuburg Austria Present address: Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences/Ume~. Plant Science Center 901 83 UmeaSweden

The sessile life style of plants creates the need to deal with an often adverse environment, in which water availability can change on a daily basis, challenging the cellular physiology and integrity. Changes in os- motic conditions disrupt the equilibrium of the plasma membrane： hypoosmotic conditions increase and hyperosmotic environment decrease the cell volume. Here, we show that short-term extracellular osmotic treatments are closely followed by a shift in the balance between endocytosis and exocytosis in root mer- istem cells. Acute hyperosmotic treatments （ionic and nonionic） enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis, whereas hypoosmotic treatments have the opposite effects. In addition to clathrin recruitment to the plasma membrane, components of early endocytic trafficking are essential during hyperosmotic stress responses. Consequently, growth of seedlings defective in elements of clathrin or early endocytic machinery is more sensitive to hyperosmotic treatments. We also found that the endocytotic response to a change of osmotic status in the environment is dominant over the presum- ably evolutionary more recent regulatory effect of plant hormones, such as auxin. These results imply that osmotic perturbation influences the balance between endocytosis and exocytosis acting through clathrin- mediated endocytosis. We propose that tension on the plasma membrane determines the addition or removal of membranes at the cell surface, thus preserving cell integrity.

关键词： osmotic stress protein trafficking clathrin-mediated endocytosis auxin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Reciprocal effects of alpha-synuclein aggregation and lysosomal homeostasis in synucleinopathy models

引用

Translational Neurodegeneration 2023年第1期12卷 479-499页

作者： Alice Drobny Fanni Annamária Boros Denise Balta Susy Prieto Huarcaya Deniz Caylioglu Niyeti Qazi Julia Vandrey Yanni Schneider Jan Philipp Dobert Caleb Pitcairn Joseph Robert Mazzulli Friederike Zunke Department of Molecular Neurology University Hospital ErlangenFriedrich-Alexander University Erlangen-Nürnberg91054 ErlangenGermany Institute of Biochemistry Christian-Albrechts-University KielKielGermany The Ken and Ruth Davee Department of Neurology Northwestern University Feinberg School of MedicineChicagoIL 60611USA.

Background Lysosomal dysfunction has been implicated in a number of neurodegenerative diseases such as Parkinson’s disease(PD).Various molecular,clinical and genetic studies have highlighted a central role of lysosomal pathways and proteins in the pathogenesis of *** PD pathology the synaptic protein alpha-synuclein(αSyn)converts from a soluble monomer to oligomeric structures and insoluble amyloid *** aim of this study was to unravel the effect ofαSyn aggregates on lysosomal turnover,particularly focusing on lysosomal homeostasis and *** these enzymes have been shown to be directly involved in the lysosomal degradation ofαSyn,impairment of their enzymatic capacity has extensive *** We used patient-derived induced pluripotent stem cells and a transgenic mouse model of PD to examine the effect of intracellularαSyn conformers on cell homeostasis and lysosomal function in dopaminergic(DA)neurons by biochemical *** We found impaired lysosomal trafficking of cathepsins in patient-derived DA neurons and mouse models withαSyn aggregation,resulting in reduced proteolytic activity of cathepsins in the *** a farnesyltransferase inhibitor,which boosts hydrolase transport via activation of the SNARE protein ykt6,we enhanced the maturation and proteolytic activity of cathepsins and thereby decreasedαSyn protein *** Our findings demonstrate a strong interplay betweenαSyn aggregation pathways and function of lysosomal *** appears thatαSyn directly interferes with the enzymatic function of cathepsins,which might lead to a vicious cycle of impairedαSyn degradation.

关键词： Dopaminergic neurons iPSC-derived models Lysosome Parkinson’s disease protein trafficking Synucleinopathy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Multiple functions of the vacuole in plant growth and fruit quality

引用

Molecular Horticulture 2021年第1期1卷 165-177页

作者： Yu-Tong Jiang Lu-Han Yang Ali Ferjani Wen-Hui Lin Joint International Research Laboratory of Metabolic&Developmental Sciences School of Life Sciences&BiotechnologyJoint Center for Single Cell BiologyShanghai Jiao Tong UniversityShanghai 200240China Department of Biology Tokyo Gakugei UniversityKoganei-shi 184-8501Japan

Vacuoles are organelles in plant cells that play pivotal roles in growth and developmental *** main functions of vacuoles include maintaining cell acidity and turgor pressure,regulating the storage and transport of substances,controlling the transport and localization of key proteins through the endocytic and lysosomal-vacuolar transport pathways,and responding to biotic and abiotic ***,proteins localized either in the tonoplast(vacuolar membrane)or inside the vacuole lumen are critical for fruit *** this review,we summarize and discuss some of the emerging functions and regulatory mechanisms associated with plant vacuoles,including vacuole biogenesis,vacuole functions in plant growth and development,fruit quality,and plant-microbe interaction,as well as some innovative research technology that has driven advances in the ***,the functions of plant vacuoles are important for plant growth and fruit *** investigation of vacuole functions in plants is of great scientific significance and has potential applications in agriculture.

关键词： Vacuole Biogenesis Plant growth and development protein trafficking Fruit quality

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tanshinone Ⅱ-A sodium sulfonate（DS-201） enhances the activity,ex-pression and trafficking of BKCa channels independently of β1 subunit

Tanshinone Ⅱ-A sodium sulfonate（DS-201） enhances the acti...

引用

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：谭晓秋 Xiu-li CHENG Yan YANG Li YAN Jing-li GU Hui LI Xiao-rong ZENG Ji-min CAO Department of Physiology Institute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical College 泸州医学院 Key Laboratory of Medical Electrophysiology Ministry of Educationand the Institute of Cardiovascular ResearchLuzhou Medical College

Aim:Tanshinone Ⅱ-A sodium sulfonate（DS-201）,a derivative of salvia miltiorrhiza,could activate BKCa channels and induce vascular relaxation,but the underlying mechanisms are *** present1βstudy aimed to address this ***:BKCa channelαsubunit,or plus subunit,were expressed in HEK293 cells by transfecting plasmids pc DNA3.1-h Slo,Flag-h Slo-EGFP,or with p EF1-myc-hβ1,*** currents were recorded using patch-clamp technique,and the expression and trafficking of channel subunits in HEK293 cells and/or vascular smooth muscle cells（VSMCs）were detected by Western blotting,flow cytometry and confocal ***:DS-201 increased the total open probability of BKCa channels in HEK293 cells 1 subunit,and this effect was associated with β subunit or with a-expressing DS-201-induced enhancements of calcium-and voltage-dependence and delay of *** ofβ1 subunit did not affect the activation EC50 of ***-201 at80μmol/L increasedαsubunit expression without affectingβ1 subunit expression both in HEK293 cells and ***-201 enriched the membranous level ofαsubunit likely by accelerating the trafficking and suppressing the internalization ofαsubunit in HEK293 ***-201 induced cytotoxicity only at very high concentrations（≥320μmol/L）.Conclusion:DS-201 potentiates the activities of BKCa channels via acutely modulating the channel kinetics and accelerating the expression and trafficking of the pore-formingαsubunit within a longer time frame.β1-subunit does not take a role in these effects of *** study suggests that DS-201 selectively targets theα-subunit in enhancing the activities of BKCa channels.

关键词： Tanshinone BKCa channel protein trafficking vascular smooth muscle cell

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

A cytoplasmic barrier in axonal initial segment selectively attenuates anterograde protein mobility in mature axon:an in vivo study

A cytoplasmic barrier in axonal initial segment selectively ...

引用

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Wang DONG~1,Mu-Ming POO~(1,2),Xiao-Hui ZHANG~1 1 Institute of Neuroscience,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 2 Division of Neurobiology,Department of Molecular & Cell Biology,Helen Wills Neuroscience Institute,University of California,Berkeley,CA 94720,USA

＜正＞The biophysical properties of intracellular diffusion and trafficking of soluble proteins in the axon of a living organism remains largely unknown,in spite of numerous findings from the cultured *** the present study,we have used in vivo time-lapse imaging and fluorescent recovery after photobleaching（FRAP） to study the mobility of diffusion of green fluorescence protein（GFP） and active transportation of vesicle associate membrane protein 2（VAMP2-GFP） in the axon of Mauthner cells and motoneurons in zebrafish *** found that a diffusion barrier emerged in the cytoplasm of axonal initial segment（IS）,by comparing the recovery rate of GFP and VAMP2-GFP at the IS and main shaft of the axon after the *** barrier was susceptible to latrunculin A,which is known to disrupt the actin cytoskeleton in the ***,the VAMP2-GFP trafficking rate was significantly faster in the anterograde direction than that for retrograde ***,our results suggest that the actin-based cytoplasmic barrier may exist in the axonal IS and attenuate the mobility of anterograde diffusion or active transportation of axonal proteins.

关键词： Axonal initial segment zebrafish protein trafficking VAMP2

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：