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Effects of dietary protein level on nutrients digestibility and reproductive performance of female mink(Neovison vison)during gestation

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Animal Nutrition 2015年第2期1卷 65-69页

作者： Qingkui Jiang Guangyu Li Tietao Zhang Haihua Zhang Xiuhua Gao Xiumei Xing Jiaping Zhao Fuhe Yang State Key Laboratory of Special Economic Animal Molecular Biology Institute of Special Economic Animal and Plant Science Chinese Academy of Agricultural Sciences Feed Research Institute Chinese Academy of Agricultural Sciences

The objective of this study was to determine whether nutrient digestibility and reproductive performance of pregnant mink(Neovison vison) were affected by different dietary protein levels. One hundred and twenty female mink were randomly assigned to four groups, receiving diets of fresh material with different protein levels. The dietary protein levels,expressed as percentage of dry matter(DM),were 32,36, 40 and 44% respectively. These values corresponded to average 320, 360, 400 and 440 g protein/kg DM, respectively. Results were as follows. All of crude protein digestibility, nitrogen(N) intake, N retention increased along with dietary protein level increasing. Low protein level(32%) significantly reduced the above indicators(P protein level. Results of mated females, barren females, kids per litter, live born kids per mated female, birth survival rate, and birth weight showed that mink achieved optimal reproductive performance when dietary protein level was 36%. In conclusion, dietary protein was anticipated to significantly influence some nutrients' utilization. Adopting the appropriate dietary protein level allow better reproduction performance. The most preferable reproductive performance was achieved when diet contained 275.5 g digestible protein per kg DM for female mink in gestation.

关键词： Female mink protein level Gestation Reproductive performance

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Fecal scores and microbial metabolites in weaned piglets fed different protein sources and levels

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Animal Nutrition 2018年第1期4卷 31-36页

作者： Xiaolu Wen Li Wang Chuntian Zheng Xuefen Yang Xianyong Ma Yunpeng Wu Zhuang Chen Zongyong Jiang Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of AgricultureState Key Laboratory of Livestock and Poultry BreedingGuangdong Public Laboratory of Animal Breeding and NutritionGuangdong Key Laboratory of Animal Breeding and NutritionInstitute of Animal ScienceGuangdong Academy of Agricultural Sciences Agro-Biological Gene Research Center Guangdong Academy of Agricultural Sciences

This experiment studied the effects of dietary protein sources and levels on the gut health of piglets,p H value,and concentrations of microbial metabolites(ammonia-N,volatile fatty acids [VFA],and polyamines) in the distal colonic and proximal colonic digesta of piglets weaned at 21 d of age.A total of 150 early-weaned piglets were allotted randomly to 5 diets: 1) control diet(CT; 17% CP),2) CT formulated with more soy protein concentrate(SPC19; 19% CP),3) more fish meal(FM19; 19% CP),4) CT formulated with more soy protein concentrate(SPC23; 23% CP),and 5) more fish meal(FM23; 23%CP).Results showed high protein level increased fecal score(P protein sources did not(P > 0.05).The p H value and ammonia-N concentration of digesta in the proximal and distal colon of FM23 were significantly higher(P 0.05).Histamine and spermidine concentrations of FM23 were higher than those of other treatments(P < 0.05).Propionic acid and butyric acid concentrations in the distal colon were higher of FM23 than of FM19(P < 0.05); putrescine,histamine and spermidine were higher of FM23 than of LP and FM19(P < 0.05).It was concluded that high dietary CP content increased microbial metabolites(ammonia-N,histamine,putrescine) in colonic digesta and aggravated piglets' diarrhea.

关键词： Piglet protein level protein source Microbial metabolites Diarrhea

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Effects of Different protein levels on the Growth Performance and Metabolic Rate of Nutrition in Broilers

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Journal of Northeast Agricultural University(English Edition) 2009年第3期16卷 27-31页

作者： WUHongda Animal Husbandry Research Center Heilongjiang Academy of Agricultural Sciences Harbin 150086 China

The objective of this study is to determine the effect of different protein levels on the growth performance and metabolic rate of nutrition in broilers. Total 360 healthy and weight closed local broilers of 3 weeks were chosen and then divided into three groups randomly by one factor. Each group contains three handlings, each handling consists of 40 broilers. The period of experiment was 7 weeks. The metabolic experiment was performed at the 7th week. Three different protein levels were used in broilers＇ diet. The levels of protein were 19%, 17.5% and 16%. The results showed that the different levels of protein in crude dietary had significant difference between 19% group and the other two groups. The average daily weight gain and daily efficiency were significantly higher than that of the other two groups （P〈0.05）. Compared to protein levels of 19% and 16%, there was no significant difference in the metabolism of calcium （P〉0.05）, and the metabolic rate of the impact of phosphorus was significantly different （P〈0.05）. The result showed that when protein level was 19%, the growth of the local broiler was the best.

关键词： protein level broiler metabolic function

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Repressed function of the C terminal domain of GS3 in grain size regulation

Repressed function of the C terminal domain of GS3 in grain ...

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2016年全国植物生物学大会

作者： Shengyuan Sun Yidan Ouyang Qifa Zhang National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

Grain yield in many cereal crops is largely determined by grain size. Our group previously established that GS3-4 allele showing stronger effect on reducing grain size due to its deficiency of the C terminal cysteine-rich domain,compared with the GS3-1 allele. Here show that the cysteine-rich domain cloud not interact with the N terminal(OSR) domain,excluding the possibility of head–to-tail interaction to block the function of OSR. We detected the protein level of the GS3-1 and GS3-4 transgenic plant which under ubiquitin promoter and fused with Flag tag in the C terminal. As expect,the enhanced phenotype mediated by overexpressing GS3-4 was correlated with the increased protein level but not m RNA level. Accumulation of GS3-1 protein but not GS3-4 protein was detected compared with the untreated control when treated with the proteasome inhibitor MG-132,suggesting that GS3-1 might degrade via C terminal domain through the proteasome-mediated pathway. These findings added to the understanding of the molecular mechanism with respect to GS3 in grain size regulation.

关键词： grain size GS3 gene OSR cysteine-rich domain protein level

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human Arrest-defective-1 (hARD 1) protein is overexpressed in breast cancer in vivo and promotes MCF-7 cell proliferation

human Arrest-defective-1 (hARD 1) protein is overexpressed i...

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第九届西南三省一市生物化学与分子生物学学术交流会

作者：马明星黄超余敏赖建华杨慧黄惟巍谭德勇云南大学生命科学学院生物化学与分子生物学实验室云南省第一人民医院病理科

The identification and characterization of genes highly expressed in cancer tissues can indicate a potential role in vivo and warrants an evaluation as useful biomarker in cancer diagnosis and/or therapy development.To this purpose,we made use of an immunological approach and showed that breast cancer cells express a significantly elevated level of arrest-defective-1(ARD1) protein in vivo.Western

关键词： ARD1 protein level breast cancer immunohistochemisty cell proliferation

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