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Journal of Northeast Agricultural University(English Edition) 1995年第2期2卷 89-95页

作者： LiuXiaobing LiWenxiong 等 HeiiongjiangInstituteofAgriculturalModernization TheChineseAcademyofSciencesHarbin150040PRC NortheastAgriculturalUniversity Harbin150030PRC

The accumulation of protein fractions was analyzed on developing and mature wheat grains of three cultivars differing in protein content and baking quality. There was a slight difference in the accumulation of cytoplasmic proteins in the cultivars used. The high yield but low protein cultivar showed a consistent decline of protein content during grain filling but the high - protein cultivars increased their psotein contant after 25 days post-anthcsis. The accumulation of storage proteins was different from that of cytoplasmic protein, and there were also cultivar variations. However, all cultivars reached their, maximum-synthesizing capacity for storage proteins at maturity. The relationship between the protein fractions or their ratio and baking quality was also discussed.

关键词： wheat protein fractions baking quality

Functional properties of sesame (Sesamum indicum Linn) seed protein fractions

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Food Production, Processing and Nutrition 2021年第1期3卷 32-47页

作者： Atinuke O.Idowu Adeola M.Alashi Ifeanyi D.Nwachukwu Tayo N.Fagbemi Rotimi E.Aluko Department of Food Science and Technology College of Basic and Applied SciencesMountain Top UniversityKm12Lagos-Ibadan ExpresswayMakogi-ObaOgun StateNigeria Department of Food and Human Nutritional Sciences University of Manitoba190 Dysart RoadWinnipegMB R3T 2N2Canada Center for Nutrition&Healthy Lifestyles School of Public HealthLoma Linda University24951 North Circle DriveLoma LindaCA 92350USA Department of Food Science and Technology Federal University of TechnologyAkureP.M.B.704AkureNigeria Richardson Centre for Functional Foods and Nutraceuticals University of Manitoba196 Innovation DriveWinnipegMB R3T 2N2Canada

This work evaluated the functional properties of sesame protein fractions in order to determine their potential in food *** seed protein fractions were prepared according to their solubility:water-soluble(albumin),salt-soluble(globulin),alkaline-soluble(glutelin)and ethanol-soluble(prolamin).Globulin was the most abundant fraction,consisting of 91%protein,followed by glutelin,albumin and prolamin in decreasing ***-reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)showed polypeptides of sizes≥20 kDa for albumin while glutelin and globulin had similar polypeptide sizes at 19,85 and 100 *** had polypeptide sizes 20,40 and 100 *** albumin and globulin fractions had higher intrinsic fluorescence intensity(FI)values than the *** had a higher solubility(ranging from 80 to 100%)over a wide pH range when compared with the other *** holding capacity(g/g)reduced from 2.76(glutelin)to 1.35(prolamin)followed by 0.42(globulin)and 0.08(albumin).Oil holding capacity(g/g)reduced from:4.13(glutelin)to 2.57(globulin)and 1.56,1.50 for albumin and prolamin *** ability was stronger for prolamin and glutelin than albumin and globulin,while higher emulsion(p<0.05)quality was obtained for prolamin and albumin than for glutelin and globulin.

关键词： Sesame seed protein fractions Functional properties Foaming capacity SDS-PAGE

Abnormalities of Serum protein fractions in Hemodialysis Patients with Chronic Renal Failure at Ouagadougou, Burkina Faso

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Advances in Biological Chemistry 2022年第6期12卷 196-206页

作者： Arnaud Kouraogo Fabienne Soudre Alice Kiba Raoul Karfo Ollo Da Emmanuel Zongo Elie Kabre Jean Sakande Biochemistry Laboratory of Souro Sanou Teaching Hospital Nazi Boni University Bobo-Dioulasso Burkina Faso Health Sciences Training and Research Unit Joseph Ki-Zerbo University Ouagadougou Burkina Faso

Introduction: The main objective of this study was to investigate abnormalities of serum protein fractions in hemodialysis patients with chronic renal failure (CRF) in Ouagadougou, Burkina Faso. Methods: This was a descriptive cross-sectional study of 48 hemodialysis patients with chronic renal failure (CRF) recruited at the Yalgado Ouedraogo Teaching Hospital (YO-TH), and 48 controls declared fit to donate blood by the Regional Blood Transfusion Center (RBTC) of Ouagadougou. Urea, creatinine, uric acid, and serum proteins were measured on the ARCHITECT C4000 equipment (ABOTT®), while the separating of the different protein fractions was performed on the Helena SAS 3 & 4 automated system. Results: A total of 96 individuals were included in the study. protein levels were on average higher in controls (75.19 ± 6.56 g/L) than in hemodialysis patients (71.44 ± 12.33 g/L). Low blood albumin was significantly associated with the CRF hemodialysis groups compared to controls (p Conclusion: Serum protein electrophoresis is rapidly feasible and low cost. In hemodialysis CKD patients, it can be used to guide therapeutic management and predict morbidity and mortality related to variations in the various protein fractions.

关键词： protein fractions Albumin Globulin Hemodialysis

Effects of sowing methods on nitrogen compounds and protease activities of whole‐crop wheat silage

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Grassland Research 2023年第1期2卷 46-55页

作者： Liuxing Xu Guojian Tang Dan Wu Jianguo Zhang College of Agronomy and Life Sciences Zhaotong UniversityZhaotongChina Department of Grassland Science South China Agricultural UniversityGuangzhouChina

Background:Whole‐crop wheat(Triticum aestivum)has high nutritive value,and it has become one of the main sources of roughage for ruminants in some countries or *** study investigates the effects of no tillage on nitrogen compounds and protease activities of whole‐crop wheat ***:Wheat was planted on the 9th day(NB9)and 5th day(NB5)before rice harvest and on the first day after rice *** before harvest involved no tillage and sowing after rice harvest involved either no tillage(NA1)or conventional tillage(CK).Results:Compared to CK,the crude protein content of NB9 and NB5 decreased by 16.4%and 9.58%,*** the delay of the sowing date,the contents of non‐protein nitrogen,rapidly degraded protein,and slowly degraded protein in whole‐plant wheat tended to *** to NA1 wheat silage,the NH_(3)‐N content of NB9 and NB5 silages decreased by 52.7%and 34.4%,*** acid protease activity of NA1 was significantly higher than that of other treatments(pprotein degradation between NA1 and CK *** the degree of protein degradation in early sowing was low,the degree of fermentation was also weak.

关键词： nitrogen distributions no‐tillage protein fractions silage whole‐crop wheat

Fertilization Management for Improving Quality Properties of Spring Wheat in Northeast China

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Agricultural Sciences in China 2005年第3期4卷 207-213页

作者： LIUXiao-bing ZHANGQiu-ying[ JINJian WANGGuang-hua SJHerbert NortheastInstituteofGeographyandAgroecology ChineseAcademyofSciencesHarbin150040P.R.China DepartmentofPlantandSoilSciences UniversityofMassachusettsAmherstMA01003USA

Fertilization management to improve quality properties of spring wheat cultivars has received little research attention inNortheast China. In this study, the effects of different fertilization management regimes on the quality properties of springwheat cultivar New Kehan 9 (Triticum aestivum L.) were investigated for two years. The results showed that fertilizationconsistently increased wheat yield, and the highest yield was obtained with addition of N, P and NPK fertilizers. The NPKtreatment resulted in 50% more yield than the unfertilized plot. The average increases in protein content from NPK and NP atseeding + N at anthesis over no fertilization and NP fertilizers at seeding were 2.7% and 0.90% respectively. The highestprotein yields were achieved in NPK and NPK + N treatments, and the lowest protein yield was observed in the no fertilizertreatment due to both low protein content and grain yield. Fertilization increased gliadins content, but decreased gluteninscontent, thus the gliadins/glutenins ratios were higher in the fertilization treatments. The most obvious effect of fertilizationon kernel quality was the significant increase of hardness percentage. Although the dough rheological properties werenot strongly changed by fertilization, dry gluten and wet gluten were significantly increased, and the highest breadvolume and bread score were found in the NPK treatment in both years. The application of 3% urea at anthesis, or applying45 kg ha-1 of potassium sulphate at seeding, with urea and diammonium phosphate as basal applications, significantlyincreased protein yield and improved quality properties of this wheat *** words: Fertilization, Yield, protein contents, protein fractions, Quality, Spring Wheat

关键词： Fertilization Yield protein contents protein fractions Quality Spring Wheat

Characterization of Seed Storage proteins in Eight Bambara Groundnut Landraces in Burkina Faso

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Agricultural Sciences 2023年第9期14卷 1268-1276页

作者： Assita Traoré-Barro Emmanuel A. M. Thiombiano Karidiatou Gnankambary Mounyratou Rabo Orokia Coulibaly Salimata Traoré Oumar Traoré Laboratoire National de Biosécurité Agence Nationale de Biosécurité Ouagadougou Burkina Faso Laboratoire de Biochimie et de Chimie Appliquée Université Joseph Ki-Zerbo Ouagadougou Burkina Faso Laboratoire de Génétique et amélioration des plantes (LAGAMEP) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso Laboratoire de protection et défense des cultures (LAPRODEC) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso

The Bambara groundnut Vigna subterranea (L.) Verdc. is a drought-resistant indigenous African grain legume with significant nutritional and agronomic potential. This study aimed to characterize the seed storage proteins of eight Bambara groundnut landraces. Seeds of Bambara groundnut landraces were collected from local markets in Burkina Faso, and total soluble protein as well as protein fractions were extracted. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content of the seeds was found to be 18.46%, with variations ranging from 17.69% to 19.17% among the different landraces. Most of the protein content was soluble, constituting approximately 87.04% of the total crude protein. Albumin fraction was the most dominant, representing about 95.42% of the total soluble proteins. The globulin, prolamin and glutelin fractions accounted for 1.82%, 0.13% and 1.17% of the soluble proteins, respectively. The findings provide valuable insights into the protein composition of Bambara groundnut landraces and contribute to our understanding of its nutritional potential, laying the groundwork for further research on crop improvement and sustainable agriculture practices.

关键词： Bambara Groundnut Landraces protein protein fractions

protein Profiles of Pod Borer Maruca Resistant Transgenic Cowpea

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American Journal of Plant Sciences 2023年第12期14卷 1453-1463页

作者： Mounyratou Rabo Teyioue Benoit Joseph Batieno Assita Traoré-Barro Salimata Traoré Orokia Coulibaly Aboubacar Toguyeni Chantal Kaboré-Zoungrana Oumar Traoré Laboratoire National de Biosécurité Agence Nationale de Biosécurité (ANB) Ouagadougou Burkina Faso Institut de l’Environnement et Recherches Agricoles Département Productions Végétales Ouagadougou Burkina Faso Laboratoire d’Etudes et de Recherches sur les Ressources Naturelles et les Sciences de l’Environnement (LERNSE) Université Nazi BONI Bobo-Dioulasso Burkina Faso Laboratoire de Génétique et amélioration des plantes (LAGAMEP) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso Laboratoire de protection et défense des cultures (LAPRODEC) Institut de l’Environnement et Recherches Agricoles Ouagadougou Burkina Faso

The grain legume cowpea Vigna unguiculata (L.) Walp. is a major protein source used for food and feed in Sub-Saharan Africa. The crop is affected by the pod borer Maruca vitrata against which transgenic lines were developed as part of the genetic control approach. This study aimed to assess the protein profiles in seeds and leaves of transgenic cowpea lines and their non-transgenic near-isogenic counterparts. Crude protein content was determined by the Kjeldahl method, and soluble proteins were quantified using Bradford dye binding assay. The average crude protein content ranged between 21.61% and 26.58% in the seeds and between 10.86% and 17.90% in the leaves. Total solubility varied between 13.03% and 20.64%. Osborne’s protein fractions contents in the seeds were 52.41% - 69.52% (albumin), 4.62% - 7.19% (globulin), 7.95% - 11.40% (glutelin) and 3% - 4% (prolamin). In any case, protein content differed significantly between cowpea genotypes but not between pairs of transgenic/non-transgenic lines. Insecticidal Cry1Ab protein expressed by transgenic lines was only detected in the albumin and globulin fractions. Altogether, these findings enhance our understanding of the effects of genetic modification on cowpea protein content and composition, with potential implications for nutritional and safety assessments.

关键词： Cowpea protein Cry1Ab protein fractions