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protein adsorption AND CELL ADHESION ON RGD-FUNCTIONALIZED SILICON SUBSTRATE SURFACES

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Chinese Journal of Polymer Science 2013年第3期31卷 495-502页

作者： Wei-fang Tong Xiao-li Liu Fei Pan 武照强 Wen-wen Jiang Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application Department of Polymer Science and EngineeringCollege of ChemistryChemical Engineering and Materials ScienceSoochow University College of Chemistry and Material Sciences South-Central University for Nationalities

A method was developed to modify silicon surfaces with good protein resistance and specific cell attachment. A silicon surface was initially deposited using a block copolymer of N-vinylpyrrolidone （NVP） and 2-hydroxyethyl methacrylate （HEMA）（PVP-b-PHEMA） film through surface-initiated atom transfer radical polymerization and then further immobilized using a short arginine-glycine-aspartate （RGD） peptide. Our results demonstrate that the RGD-modified surfaces （Si-RGD） can suppress non-specific adsorption of proteins and induce the adhesion of L929 cells. The Si-RGD surface exhibited higher cell proliferation rates than the unmodified silicon surface. This research established a simple method for the fabrication of dual-functional silicon surface that combines antifouling and cell attachment promotion.

关键词： Cell adhesion protein adsorption RGD Poly（N-vinylpyrrolidone） ATRP.

protein adsorption, cell viability and corrosion properties of Ti6Al4V alloy treated by plasma oxidation and anodic oxidation

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International Journal of Minerals,Metallurgy and Materials 2020年第9期27卷 1269-1280页

作者： ÖzgüBayrak Hojjat Ghahramanzadeh Asl Ayşe Ak Department of Mechanical Engineering Erzincan Binali Yıldırım UniversityErzincan 24100Turkey Department of Mechanical Engineering Karadeniz Technical UniversityTrabzon 61080Turkey Vocational School of Health Services Medical Imaging Techniques ProgrammeKocaeli UniversityKocaeli 41001Turkey

The hardness,wettability,and electrochemical properties of Ti6Al4V alloy surfaces treated with anodic oxidation and plasma oxidation as well as the viabilities of the different cell lines on the obtained surfaces were investigated.The anodic oxidation was performed for 10 min under 100 V potential,and it resulted in a 0.95μm thick nanoporous anatase-TiO2 structure.On the other hand,plasma oxidation was carried out at 650℃ for 1 h and resulted in a dense rutile-TiO2 structure with a thickness of 1.2μm.While a hardness of HV0.025823 and roughness of^220 nm were obtained by plasma oxidation,those obtained by anodic oxidation were HV0.025512 and^130 nm,respectively.The anodic oxidation process created a more hydrophilic surface with a contact angle of 87.2°.Both oxidation processes produced similar properties in terms of corrosion behavior and showed better resistance than the as-received state in a certain range of potential.Moreover,the surface treatments led to no significant change in the protein adsorption levels,which indicates that the difference in viability between the osteoblast and fibroblast cells was not due to the difference in surface protein adsorption.Given all the factors,the surfaces obtained by anodic oxidation treatment revealed higher cell viability than those obtained by plasma oxidation(p=0.05).

关键词： Ti6Al4V oxidation corrosion cell viability protein adsorption

protein adsorption behaviors on chitosan/poly(ε-caprolactone) blend films studied by quartz crystal microbalance with dissipation monitoring(QCM-D)

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Science China(Technological Sciences) 2009年第8期52卷 2275-2279页

作者： ZENG Rong ZHANG Yi LIANG ZhiHong TU Mei ZHOU ChangRen Department of Materials Science and Engineering College of Science and EngineeringJinan UniversityGuangzhou 510632China Engineering Research Center of Artificial Organs and Materials Ministry of EducationGuangzhou 510632China Experiment and Technology Center Jinan UniversityGuangzhou 510632China

Chitosan/poly(ε-caprolactone) (PCL) blend films in different mass ratios were prepared using the chitosan/PCL mixture solutions in 80 vol.-% acetic acid by spin coating. Their surface micromorphologies were assessed by atomic force microscopy (AFM). It was found that the micromorphology of chitosan/PCL blend films was in large extent related to the mass ratio of chitosan. 25 wt% chitosan/PCL blend film presented microphase separation. The protein adsorption of bovine serum albumin (BSA) onto chitosan/PCL blend films was investigated by using quartz crystal microbalance with dissipation monitoring (QCM-D) in real time. The results suggested that the amount of adsorbed BSA on the chitosan/PCL blend films decreased with the addition of chitosan, but the structure and viscoelastic properties of the adsorbed BSA layers were greatly affected by the surface micromorphology of chitosan/PCL blend films. BSA absorbed on the 25 wt% chitosan/PCL blend film with microphase separa- tion showed larger adsorption reversibility, and preferred to form a loose, dissipative layer in comparison with those on other chitosan/PCL blend films without microphase separation.

关键词： protein adsorption chitosan poly(ε-caprolactone) quartz crystal microbalance with dissipation monitoring(QCM-D) surface micromorphology

Advanced protein adsorption properties of a novel silicate-based bioceramic: A proteomic analysis

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Bioactive Materials 2021年第1期6卷 208-218页

作者： Fanyan Deng Wanyin Zhai Yue Yin Chao Peng Congqin Ning State Key Laboratory of High Performance Ceramics and Superfine Microstructure Institute of CeramicsChinese Academy of SciencesShanghai200050China The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials Shanghai Normal UniversityShanghai200234China National Facility for protein Science in Shanghai Zhangjiang LabShanghai Advanced Research InstituteChinese Academy of ScienceShanghai201210China

Silicate bioceramics have been shown to possess excellent cytocompatibility and osteogenic activity,but the exact mechanism is still unclear.protein adsorption is the first event taking place at the biomaterial-tissue interface,which is vital to the subsequent cellular behavior and further influence the biomaterial-tissue interaction.In this work,the protein adsorption behavior of a novel CPS bioceramic was evaluated using the proteomics technology.The results showed that CPS adsorbed more amount and types of serum proteins than HA.FN1 and IGF1 proteins selected from proteomics results were validated by Western-blot experiment.Pathway analysis also revealed mechanistic insights how these absorbed proteins by CPS help mediate cell adhesion and promotes osteogenic activity.Firstly,the dramatically enhanced adsorption of FN1 could greatly promote cell adhesion and growth.Secondly,IGF1 was uniquely adsorbed on CPS bioceramic and IGF1 could activate Rap1 signaling pathway to promote cell adhesion.Thirdly,the increased adsorption of FN1,IGF1 and COL1A2 proteins on CPS explains its better ability on bone regeneration than HA.Fourthly,the increased adsorption of IGF1,CHAD,COL2A1 and THBS4 proteins on CPS explains its ability on cartilage formation.Lastly,the increased adsorption of immunological related proteins on CPS may also play a positive role in bone regeneration.In addition,CPS had a much better cell adhesion ability than HA,proving that more adsorbed proteins really had a positive effect on cell behavior.The more adsorbed proteins on CPS than HA might indicated a better bone regeneration rate at early stage of implantation.

关键词： Silicocarnotite Bioceramic protein adsorption Proteomics Pathway

POLY(N-VINYLPYRROLIDONE)-MODIFIED SURFACES REPEL PLASMA protein adsorption

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Chinese Journal of Polymer Science 2012年第2期30卷 235-241页

作者：武照强 College of Chemistry Chemical Engineering and Materials ScienceSoochow UniversitySoochow 215123China

The present work aimed to study the interaction between plasma proteins and PVP-modified surfaces under more complex protein conditions. In the competitive adsorption of fibrinogen （Fg） and human serum albumin （HSA）, the modified surfaces showed preferential adsorption of HSA. In 100% plasma, the amount of Fg adsorbed onto PVP-modified surfaces was as low as 10 ng/cm2, suggesting the excellent protein resistance properties of the modified surfaces. In addition, immunoblots of proteins eluted from the modified surfaces after plasma contact confirmed that PVP-modified surfaces can repel most plasma proteins, especially proteins that lalav important roles in the nrocess of blood coagulation.

关键词： Poly（N-vinylpyrrolidone） Surface modification protein adsorption ATRP Biocompatibility.

Plasma graft of poly(ethylene glycol) methyl ether methacrylate (PEGMA) on RGP lens surface for reducing protein adsorption

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Plasma Science and Technology 2017年第1期19卷 51-56页

作者：尹诗衡任力王迎军 Analytical ＆ Testing Center South China University of Technology Guangzhou 510640 People's Republic of China College of Materials Science and Engineering South China University of Technology Guangzhou 510640 People's Republic of China

Poly（ethylene glycol） methyl ether methacrylate（PEGMA） was grafted on fluorosilicone acrylate rigid gas permissible contact lens surface by means of argon plasma induced polymerization to improve surface hydrophilicity and reduce protein adsorption.The surface properties were characterized by contact angle measurement,x-ray photoelectron spectroscopy（XPS） and atomic force microscopy respectively.The surface protein adsorption was evaluated by lysozyme solution immersion and XPS analysis.The results indicated that a thin layer of PEGMA was successfully grafted.The surface hydrophilicity was bettered and surface free energy increased.The lysozyme adsorption on the lens surface was reduced greatly.

关键词： RGP contact lens PEGMA plasma graft polymerization protein adsorption

SURFACE MODIFICATION OF TITANIUM FILMS WITH SODIUM ION IMPLANTATION:SURFACE PROPERTIES AND protein adsorption

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Acta Metallurgica Sinica(English Letters) 2007年第2期20卷 148-156页

作者： K. Y. Cai College of Bioengineering Chong Qing University Chongqing 400044 China

Sodium implanted titanium films with different ion doses were characterized to correlate their ion implantation parameters. Native titanium films and ion implanted titanium films were characterized with combined techniques of X-ray photoelectron spectroscopy （XPS）, atomic force microscopy （AFM）, and light microscopy （LM）. The surface presented increased sodium concentration on treated titanium films with ion dose increasing, except for the group with the highest ion dose of 4×10^17ions/cm^2. XPS depth profiling displayed that sodium entered titanium film around 25-50nm depth depending on its implantation ion dose. AFM characterization showed that sodium ion implantation treatment changed the surface morphology from a relatively smooth titanium film to rough surfaces corresponding to different implantation doses. After sodium implantation, implanted titanium films presented big particles with island structure morphology. The surface morphology and particle growth displayed the corresponding trend. Fibrinogen adsorption on these titanium films was performed to correlate with the surface properties of treated titanium films. The results show that protein adsorption on ion-implanted samples with dose of 2×10^17 and 4×10^17 are statistically higher （p 〈0.01） than samples treated with dose of 5×10^16 and 1×10^17, as well as the control samples.

关键词： titanium film sodium ion implantation protein adsorption surface characterization

Effects of proteins on magnesium degradation-static vs.dynamic conditions

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Journal of Magnesium and Alloys 2023年第4期11卷 1332-1342页

作者： Ruiqing Hou Frank Feyerabend Heike Helmholz Vasil M.Garamus Regine Willumeit-Römer School of Materials Science and Engineering&Henan Key Laboratory of Advanced Magnesium Alloys Zhengzhou UniversityNo.100 Science AvenueZhengzhou 450001China Institute of Metallic Biomaterials Helmholtz-Zentrum Hereon(Hereon)Max-Planck-Str.1Geesthacht 21502Germany

The interaction between organic molecules and biomaterial surfaces determines the fate of biomaterials during their service life,which is also the research hotspots in the field of biomaterials.To understand the mechanism of protein interaction with magnesium(Mg)degradation,alloying elements,immersion time,protein concentration and surface conditions have been previously considered for the effect of proteins on Mg degradation.However,fluid flow,as one of the critical factors,drew little attention in this case.In the present study,the effect of bovine serum albumin(BSA)and fetal bovine serum(FBS)on Mg degradation was compared under static and dynamic conditions.The results revealed that both BSA and FBS slightly decreased the degradation rate of Mg in Hanks’balanced salt solution(HBSS)under static immersion due to the protein adsorption and the formation of a Ca/P-rich top layer on Mg surface,whereas under dynamic flow condition the degradation of Mg was significantly accelerated in the presence of BSA or FBS.The reasons seemed to stem from the weakened protein adsorption on Mg surface in this case and the dynamically enhanced interaction between proteins and ions/products in solutions,which largely weaken the combination of the top Ca/P-rich layer with the inner corrosion product layer.These results highlight the importance of testing conditions for Mg characterization in vitro and the synergistic effect between different parameters on Mg degradation.

关键词： Flow Bovine serum albumin Fetal bovine serum protein adsorption

Moderated crevice corrosion susceptibility of Ti6Al4V implant material due to albumin-corrosion interaction

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Journal of Materials Science & Technology 2022年第14期109卷 209-220页

作者： Jing Wu Meng Li Chuanchuan Lin Pengfei Gao Rui Zhang Xuan Li Jixi Zhang Kaiyong Cai Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing 400044China

Localized corrosion of metallic biomaterials is a concerning problem for implant applications.In the present work,the interaction between localized corrosion of Ti6Al4V implant material and adsorption of bovine serum albumin(BSA)was investigated by electrochemical tests,surface morphological and compositional analysis.The artificial crevice was set up on the Ti6Al4V surface to simulate the crevice corrosion of metallic implants.The results show that the BSA adsorption is regulated by the heterogeneous metallic ion release resulted from the crevice corrosion.The negatively charged BSA prefers to aggregate at the interior of artificial crevice due to electrostatic interaction with the concentrated metallic cations in the crevice.In return,the non-uniform BSA adsorption can decrease the crevice corrosion susceptibility of the Ti6Al4V implant material,even though the presence of BSA accelerates the dissolution of passive films.For the Ti6Al4V alloys with artificial crevice,stable localized corrosion occurs after 96 h of immersion in the PBS free of BSA while no stable localized corrosion can be determined in the presence of 4%BSA.It is deduced that the presence of BSA can reduce the surface potential discrepancy between the crevice interior and the crevice exterior.The disclosed mechanism of albumin-corrosion interaction is vital to the localized corrosion control of metallic implants in clinical use.

关键词： Titanium alloy Albumin Electrochemistry Localized corrosion protein adsorption