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Extensive Inter-plant protein transfer between Cuscuta Parasites and Their Host Plants

Molecular Plant 2020年第4期13卷 573-585页

作者： Nian Liu Guojing Shen Yuxing Xu Hui Liu Jingxiong Zhang Shalan Li Jing Li Cuiping Zhang Jinfeng Qi Lei Wang Jianqiang Wu Department of Economic Plants and Biotechnology Yunnan Key Laboratory for Wild Plant ResourcesKunming Institute of BotanyChinese Academy of SciencesKunming 650201China University of the Chinese Academy of Sciences Beijing 100049China

Cuscuta species(dodders)are holoparasites that totally rely on host plants to *** various mobile proteins have been identified to travel within a plant,whether and to what extent protein transfer between Cuscuta and host plants remain *** found that hundreds to more than 1500 proteins were transferred between Cuscuta and the host plants Arabidopsis and soybean,and hundreds of interplant mobile proteins were even detected in the seeds of Cuscuta and the host *** hosts bridge-connected by dodder were also found to exchange hundreds of ***,the mobile proteins represent a few to more than 10%of the proteomes of foreign *** Arabidopsis plants expressing different reporter proteins,we further showed that these reporter proteins could travel between plants and,importantly,retained their activity in the foreign *** analysis between the interplant mobile proteins and mRNAs indicated that the majority of mobile proteins were not de novo synthesized from the translocated mRNAs,but bona fide mobile *** propose that large-scale inter-plant protein translocation may play an important role in the interactions between host plants and dodder and even among the dodder bridge-connected hosts.

关键词： dodder Cuscuta host plants protein transfer plant-plant interaction

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Improved protein transfer Efficiency and Signal Intensity in BlotMan Using Pulse Width Modulation

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Journal of Biomedical Science and Engineering 2016年第5期9卷 269-279页

作者： Thuc Nhi Truongvo Feixue Zheng Stanley Chien Hiroki Yokota Department of Biomedical Engineering Indiana University Purdue University Indianapolis Indianapolis IN USA YC Bioelectric LLC Indianapolis IN USA Department of Electrical and Computer Engineering Indiana University Purdue University Indianapolis Indianapolis IN USA

BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.

关键词： Pulse Width Modulation (PWM) Western Blotting protein transfer

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