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mg132 Induced Apoptosis Pathway in HL-60 Cells and Impact of Allogeneic Mixed Lymphocyte Reaction

Chinese Journal of Cancer Research 2009年第4期21卷 333-339页

作者： Yong-ming Zhou Wei Guo Hao Zhou Jin-hua Zhang Zhi-ping Liu Mei-xia Yu Department of Hematology the Affiliated Tianyou Hospital Wuhan University of Science and Technology Wuhan 430064China Department of Hematology Affiliated Hospital Jiujiang University Jiujiang 33200 China Department of Endocriology Wuhan No.1 Hospital Wuhan 430032 China Department of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Objective： To investigate the proteasome inhibitor mg132-induced apoptosis pathway in HL-60 cells and the role of allogeneic mixed lymphocyte reaction. Methods： Cell apoptosis was analyzed by flow cytometry. The expressions of p21 protein, p27 protein and p53 protein in HL-60 ceils treated with mg132 were measured by Western blot. The proliferation of, peripheral blood mononuclear cells （PBMNCs） after treatment with 75 Gy irradiated HL-60 cells treated with mg132 was measured with CCK-8. Results： High-dose mg132 induced apoptosis in HL-60 cells. No significant change was observed in mg132-induced apoptosis after inhibiting caspase-8 and caspase-9 pathway. The expressions of p21 protein and p27 protein increased in mg132-induced apoptosis. HL-60 cells treated with low-dose mg132 improved the proliferation of PBMNCs from healthy volunteers. Conclusion： High-dose mg132 induced apoptosis and directly killed HL-60 ceils. mg132 induced apoptosis in a caspase-8- and caspase-9-independent pathway, p21 protein and p27 protein were involved in mg132-induced apoptosis in HL-60 cells. HL-60 cells treated with Low-dose mg132 improved the effect of promoting the proliferation of PBMNCs from healthy volunteers.

关键词： proteasome inhibitor mg132 Apoptosis p21 protein p27 protein Allogeneic mixed lymphocyte reaction

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