检索结果-南通市图书馆

Frontiers of Medicine 2016年第4期10卷 517-521页

作者： Ying Ma Nanxue Zhang Shi Wu Haihui Huang Yanpei Cao Department of Dermatology Huashan Hospital Fudan University Shanghai 200040 China Institute of Antibiotics Huashan Hospital Fudan University Shanghai 200040 China Nursing Department Huashan Hospital Fudan University Shanghai 200040 China

This study aimed to compare the antimicrobial activities of topical agents against propionibacterium acnes isolated from patients admitted to a hospital in Shanghai, China. The minimal inhibitory concentrations of the cultured P. acnes were determined in accordance with the Clinical and Laboratory Standards Institute. Susceptibilities to clindamycin and erythromycin were compared in terms of gender, age, disease duration, previous treatment, and disease severity. A total of 69 P. acnes strains were isolated from 98 patients （70.41%）. The susceptibility to triple antibiotic ointment （neomycin/bacitracin/polymyxin B） and bacitracin was 100%. The susceptibility to fusidic acid was 92.7%. The resistance rates to neomycin sulfate, erythromycin, and clindamycin were 11.7%, 49.3%, and 33.4%, respectively. The high resistance rate to clindamycin and erythromycin was significantly affected by gender, previous treatment, and disease severity rather than by age and disease duration. Topical antibiotics should not be used separately for long-term therapy to avoid multiresistance. The use of topical antibiotics should be determined by clinicians on the basis of clinical conditions.

关键词： antimicrobial susceptibility/resistance propionibacterium acnes topical antibiotics in vitro study

Unexpected positive cultures including isolation of propionibacterium acnes in revision shoulder arthroplasty

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2014年第22期127卷 3975-3979页

作者： Seung Ju Kim Jong Hun Kim Department of Orthopaedics Korea Electric Power Corporation(KEPCO)Medical Foundation KEPCO Medical center SeoulRepublic of Korea Division of Infectious Diseases Department of Internal MedicineUniversity of Utah Salt Lake City UT 84132 USA

Objective Little information is available to guide treatment strategies regarding patients with unexpected positive cultures （UPC）,including propionibacterium acnes （PA）,without overt signs of infection in revision shoulder arthroplasty （RSA）.The purpose of our study was to analyze the prevalence,clinical meaning,treatment and prognosis of UPC in *** sources and study selection We performed a systematic review of the literature between 1950 and 2013 for all studies reporting on UPC for *** with the prevalence and prognosis of patients with UPC in RSA were *** Six studies meeting our inclusion criteria yielded data for 1 402 patients who underwent a total of 1405 *** on the available data,following RSA 235 shoulders had UPCs with a pooled percentage of 16.7％（235/1405）.The most commonly isolated bacteria from shoulders following RSA with UPCs was PA with pooled percentages of 63.4％（149/235）.Occurrence of true infection from UPCs after RSA was seen in 24 shoulders （24/235,10.2％）.Antibiotic use did not influence the rate of the occurrence of true infection from UPCs （P=0.498）.Conclusions Our study showed a low risk of having a true infection from UPCs after RSA without clinical signs of preoperative infection at the time of the ***,prolonged antibiotic therapy may not be necessary in these patients.

关键词： revision shoulder arthroplasty unexpected positive culture propionibacterium acnes

In vitro antimicrobial susceptibility of propionibacterium acnes from patients with acne vulgaris in Guangzhou city of China

在线全文

学校读者我要写书评

暂无评论

International Journal of Dermatology and Venereology 2018年第1期1卷 44-48页

作者： Yue Zhao Hai- Yan Chen Ya-Ning Wang Song- Chao Yin Miao-Jian Wan Yue Zheng Rong Hu Feng-Xian Ou Qing-Ting Zeng Lei Guan Wei Lai Department of Dermatology and Venereology The 3rd Affiliated Hospital of Sun Yat-sen UniversityGuangzhou Guangdong 510630 China Skin Research Center LandproofTesting Technology Co. Ltd Guangzhou Guangdong 510635 China Laboratory of Dermatology and Venereology The 3rd Affiliated Hospital of Sun Yat-sen UniversityGuangzhou Guangdong 510630 China

ObjectiveThis study was to determine the antimicrobial susceptibility of P. acnes in acne patients in Guangzhou city of southern China. MethodsAmong 124 patients, we isolated 100 stains of P. acnes and did the antimicrobial susceptibility tests in vitro. ResultsBy applying the Clinical and Laboratory Standards Institute document M11-A7 breakpoints for resistance, 11%, 39%, 36%, 71%, 72%, 53%, 3%, 28% of the stains were resistant to minocycline, doxycycline, tetracycline, erythromycin, azithromycin, clindamycin, ceftriaxone and levofloxacin, respectively. Moreover, severe cross/multiple resistance was also observed.ConclusionThe high prevalence of antimicrobial resistant P. acnes should cause alarm and attention in Guangzhou.

关键词： propionibacterium acnes Acne vulgaris Microbial sensitivity test Drug resistance

Pathogen characteristics and its sensitivity against antimicrobial agents in fatal bacterial granuloma after eyelid trauma in vitro

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2011年第4期4卷 410-412页

作者： Xiu-Li Xu Jia-Yun Liu Tian-Wen Gao Peng-Liang Zhang Xian-Long Qi Xiao-Dong Cheng and Xiao-Ke Hao Department of Laborator and Department of Dermatology Xijing Hospital Fourth Military Medical University Xi'an 710033 Shaanxi Province China

AIM: To understand the pathogen characteristics and its sensitivity against antimicrobial agents in fatal bacterial granuloma after eyelid trauma (FBGT) in vitro, and to provide laboratory evidence for diagnosis. METHODS: The FBGT pathogens were isolaated and cultured with reformed rabbit-brain anaerobic enriched broth (RRAB), and identified by ATB/API 20A system. The minimum inhibiting concentration (MIC) was determined by anaerobic broth dilution method. RESULTS: A total of 22 strains of pathogen were separated from 21 patients with FBGT and identified as propionibacterium acnes (PA) by ATB/API 20A system. The MIC of ciprofloxacin for 22 PA strains was 0.0625-0.5mg/L, the MIC of penicillin, ampicillin, ampicillin/sulbactam, cefoperazone, lincomycin, and imipenem/cilastatin were 0.125-0.5mg/L, the MIC of ticarcillin/clavulanic acid was 0.250-1.000mg/L, and the MIC of metronidazole was 64-256mg/L. The pathogen of FBGT was strictly anaerobic PA, which growed slowly and better in nutritious RRAB broth. All PA were resistant to metronidazole, but susceptive to other routine antimicrobial agents, such as penicillin, ampicillin and lincomycin. CONCLUSION: FBGT should not be treated with metronidazole. Clinicians should choose combined use of drugs or operation to treat FBGT according to patients' individual condition and the results of drug sensitivity test.

关键词： Fatal bacteria granuloma after trauma propionibacterium acnes Antimicrobial agents Minimum inhibiting concentration

Effects of cosmetics containing purified honeybee(Apis mellifera L.) venom on acne vulgaris

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Medicine 2013年第5期11卷 320-326页

作者： Sang Mi Han Kwang Gill Lee Sok Cheon Pak Department of Agricultural Biology National Academy of Agricultural Science School of Biomedical Sciences Charles Sturt University

Acne vulgaris is a chronic dermatologic problem with multiple factors involved in its pathogenesis. Alternative solutions to acne treatment were instigated by antibiotic resistance despite of its extensive use. Purified bee venom （PBV） has been proposed as a promising candidate for that purpose. The present study was designed to confirm the antibacterial effect of PBV and access the efficacy of cosmetics containing PBV in subjects with acne vulgaris. METHODS： The skin bacterium propionibacterium acnes was incubated with PBV at various concentrations and bacterial growth was evaluated using the colony forming unit （CFU） assay. The mechanism of PBV employed in killing P. acnes was examined by scanning electron microscopy （SEM） and transmission electron microscopy （TEM）. In addition, a total of 12 subjects were randomized in a double-blind, controlled trial to receive either cosmetics containing PBV or cosmetics without PBV for two weeks. Evaluations included lesion counts and skin microorganism. RESULTS： PBV exhibited antimicrobial activity in a concentration-dependent manner, reducing the number of P. acnes CFU by approximately 6 logs at a concentration of 0.5 mg. When PBV concentration was higher than 1.0 mg, no P. acnes colonies were spotted on an agar. TEM and SEM of untreated P. acnes illustrated the normal pleomorphic structure, whereas the PBV- treated bacterium lost the integrity of surface architecture. Significant difference （P=0.027） in the grading levels based on numbers of lesion counts for inflammatory and noninflammatory was observed in favour of the PBV group compared with the control group. In terms of average decrement of skin microorganism, subjects receiving cosmetics containing PBV experienced a significant 57.5% decrease of adenosine triphosphate levels, whereas participants receiving cosmetics without PBV experienced a nonsignificant decrease of 4.7%. CONCLUSION： These results show that the in vitro actions of antimicrobial activity of PBV were

关键词： bee venoms acne vulgaris propionibacterium acnes skin cosmetics

Mass Spectrometric Identification of propionibacterium Isolates Requires Database Enrichment

在线全文

学校读者我要写书评

暂无评论

Advances in Microbiology 2012年第4期2卷 497-504页

作者： Sophie Edouard Carine Couderc Didier Raoult Pierre-Edouard Fournier Unite de Recherche sur les Maladies Infectieuses Tropicales et Emergentes(URMITE) Aix-Marseille UniversiteMarseilleFrance Federation de Microbiologie Clinique Hopital de la TimoneMarseilleFrance

propionibacterium species are mostly environmental bacteria, some being commensal of mammals including humans, and sometimes pathogenic. These bacteria are poorly identified using routine laboratory methods. Recently, Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) has emerged as a rapid and efficient method to identify bacterial species. We evaluated the use of MALDI-TOF-MS for identification of all validated propionibacterium species. Only four of the 15 tested reference strains (26.7%) were correctly identified at the species level, and P. acnes, the most common human pathogenic species was not identified. When applying MALDI-TOF-MS to 48 P. acnes strains, only 18.7% were correctly identified, suggesting an intraspecific variability of proteic profiles among propionibacterium strains. However, by enriching the Bruker database with spectra from five of these strains and re-testing the other 43 strains against this new database, 93.0% were correctly identified. Our study demonstrates that MALDI-TOF-MS may be used for the identification of propionibacterium isolates but requires a database enrichment in spectra from additional isolates.

关键词： propionibacterium propionibacterium acnes MALDI-TOF Mass Spectrometry