检索结果-南通市图书馆

programmed cell death-I/programmed death-ligand 1 Pathway： A New Target for Sepsis

Chinese Medical Journal 2017年第8期130卷 986-992页

作者： Qiang liu Chun-Sheng li Intensive Care Unit Central Hospital of Dandong City Dandong Liaoning 118002 China Department of Emergency Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Beijing Key laboratory of Cardiopulmonary Cerebral Resuscitation Beijing 100020 China

Objective： Sepsis remains a leading cause of death in many Intensive Care Units worldwide, lmmunosuppression has been a primary locus of sepsis research as a key pathophysiological mechanism. Given the important role of the negative costimulatory molecules programmed cell death-1 （PD-1） and programmed death-ligand 1 （PD-lI） in the occurrence of immunosuppression during sepsis, we reviewed literatures related to the PD-1/PD-l 1 pathway to examine its potential as a new target for sepsis treatment. Data Sources： Studies of the association between PD-I/PD-lI and sepsis published tip to January 31, 2017, were obtained by searching tile PubMed database. Study Selection： English language studies, including those based on animal models, clinical research, and reviews, with data related to PD- 1/PD-l I and sepsis, were evaluated. Results： lmmunomodulatory therapeutics could reverse the deactivation of immune cells caused by sepsis and restore immune cell activation and function. Blockade of＇the PD-1/PD-lI pathway could reduce the exhaustion ofT-cells and enhance the proliferation and activation ofT-cells. Conclusions： The anti-PD- I/PD-l 1 pathway shows promise as a new target for sepsis treatment. This review provides a basis for clinical trials and Iiiture studies aimed at revaluating the efficacy and safety of this targeted approach.

关键词： lmmunosuoDression programmed cell death-l programmed death-ligand 1 Sepsis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hepatoma cells up-regulate expression of programmed cell death-1 on T cells

引用

World Journal of Gastroenterology 2008年第44期14卷 6853-6857页

作者： Ji Chen Xue-Jie Wu Gui-Qiang Wang Department of Infectious Diseases & Center for liver Diseases Peking University First Hospital Beijing 10034 China

AIM： To investigate the effect of hepatoma cells on up-regulation of programmed cell death-1 （PD-1）, and the function of PD-1 on T cells. METHODS： HepG2 or HepG2.***.1.5 cells were cocultured with a lymphoma cell line-Jurkat cells. PD-1 expression was detected by flow cytometry. Il：2, INF-γ and Il-10 in culture supernatant were detected by enzyme-linked immunosorbent assay （ElISA）. Cytotoxic action of T cells was determined by MIF reduction assay-direct mononuclear cell cytotoxicity assay. RESUlTS： The PD-1 expression on Jurkat cells increased by 16.17% ± 2.5% and 17.43% ± 2.2% after HepG2 or HepG2.***.1.5 cells were co-cultured for 48 h. The levels of Il-2, INF-γ and Il-10 in the culture supernatant were 202.9 ＋ 53.0 pg/ml, 88.6 ± 4.6 pg/ml and 63.7± 13.4 pg/ml respectively, which were significantly higher than those （102.9 ± 53 pg/ml, 39.3 ± 4.2 pg/ml, and 34.6 =E13.7 pg/ml） in the control group （P 〈 0.05）. The OD value for MTT assay in the blocking group （0.29 ± 0.06） was significantly higher than that （0.19 ± 0.09） in the control group （P 〈 0.05）. CONClUSION： PD-1 expression on Jurkat cells is upregulated by hepatoma cells, cytokines and cytotoxic action are elevated after PD-1/PD-l1 is blocked.

关键词： Hepatoma cell programmed cell death-l Protein expression T cell function Cytokine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：