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Isolation,Identification and Antibiotics Susceptibility Test of Citrobacter freundii from procambarus clarkia

Animal Husbandry and Feed Science 2014年第5期6卷 238-241页

作者： Chen Honglian Song Guangtong He Jixiang Hou Guanjun Wang Yongjie Fisheries Research Institute Anhui Academy of Agricultural Sciences

This experiment was conducted to clarify species and drug resistance of pathogen from the diseased procambarus clarkia. Pathogenic bacteria from hepatopancreas of the diseased P. clarkia were examined using conventional methods,and then were isolated. The further tests and analysis of the isolated strain were developed,including the regression experiment to P. clarkia,the morphology,physiological and biochemical characteristics,sequence analysis of their 16 S rRNA and gyr B genes,and the susceptibility test to antibiotics. Large colonies with similar morphology and color were obtained. Strain X120523 was identified as Citrobacter freundii,proved to have strong pathogenicity,and was susceptible to quinolones and aminoglycosides.

关键词： procambarus clarkia Isolation and identification Citrobacter freundii 16S rRNA gene gyr B gene Antibiotics susceptibility test

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Observation on Behavior of procambarus clarkii and Eriocheir sinensis in Polyculture System

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Agricultural Science & Technology 2012年第3期13卷 656-660页

作者：余魁英季鹏飞鲍玉婷唐建清黄成南京大学生命科学学院江苏南京210093 南京大学金陵学院江苏南京210089 江苏省淡水水产研究所江苏南京210017

[Objective] This study aimed to observe and explore the behavior of procambarus clarkii and Eriocheir sinensis in polyculture system. [Method] P. clarkii and E. sinensis with the same weight were used for the experiment in a relatively small polyculture system. The agonistic behavior of P. clarkii and E. sinensis in the first 30 min of the initial encounter and the fighting results were observed and recorded; the approach-avoidance behavior of P. clarkii to bigger E. sinensis in a relatively large space （the ＂Y＂ maze） was observed and recorded. [Result] The re- sults showed that the times of first aggressions and approaches and the total times of active aggressions and approaches of P. clarkii in the initial period of polyculture experiment were all significantly more than E. sinensis （/：＇〈0.05）; after 30 d of poly- culture, P. clarkfi died in 30% of the groups while E. sinensis died in only 10% of the groups, and they coexisted in 60% of the groups. In the experiment of ap- proach-avoidance behavior of P. clarkii to bigger E. sinensis, with the increase of experiment times, the tendency of approach and avoidance of P. clarkii to the con- trol and E. sinensis became control-prefer （P〈0.05） from insignificantly different （P〉 0.05）, which indicated that the recognition ability of P. clarkii for E. sinensis was sig- nificantly improved, and the selection behavior of E. sinensis was obviously inhibited. [Conclusion] Presumably, polyculture of crayfishes and crabs can achieve better effi- ciency when under appropriate habitat conditions.

关键词： procambarus clarkia Eriocheir sinensis Polyculture Behavior Interspecific relationship

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Effect of 17α-hydroxyprogesterone on Synchronous Ovarian Development of Crayfish,procambarus clarkii

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Animal Husbandry and Feed Science 2014年第6期6卷 312-315页

作者： Liu Wei Chen Shuqiao Mao Jianqiang Zhang Di Zhou Guoqin Fisheries Science Research Institute of Nanjing

Three different treatment methods, including topical application method, injection method and immersion method were employed to treat 17or - hydroxyprogesterone, so as to investigating its effect on ovarian development red swamp crayfish （procambarus clarkii）. The results showed that all the three differ- ent methods could facilitate the synchronous ovarian development of red swamp crayfish at different degree, with the degree of maturity of 10% high compared with the control group. The highest maturity degree was 50% that was observed in immersion treatment group. The effect was determined as in order immersion method 〉 injection method 〉 topical apphcation method 〉 control group. The immersion method and the injection method could facilitate synchronization spawning of red swamp crayfish, with the order of immersion method 32.5%, injection method 20%, topical application 17.5% and the control group 17.5 %. Taking the synchro- nization spawning rate, final spawning rate and mortality into account, the order was the immersion method 〉 injection method 〉 topical application method 〉 control group.

关键词： procambarus clarkia Ovary Synchronization development Spawning 17ct-hydroxyprogesterone

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