检索结果-南通市图书馆

Chinese Journal of Chemistry 1999年第6期17卷 658-673,6页

作者：叶国杰卢保元金善炜钱瑞卿汪猷 Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China

The complete amino acid sequence of β-momorcharin, a ribosome-inactivating protein from the seeds of Momordica charantia Linn (Cucurbitaceae) has been determined. This has been done by the sequence analysis of peptides obtained by enzymatic digestion with trypsin, chymotrypsin and S. aureus V8 protease, as well as by chemical cleavage with BNPS-skatole. The protein consists of 249 amino acid residues containing one asparagine - linked sugar group attached to the site of Asn 5 1 and has a calculated relative molecular mass of 28,452 Da without addition of the carbohydrate. Comparison of this sequence with those of trichosanthin and other ribosome-inactivating proteins from different species of plants shows a significant homology with each other. Regarding the similarity of their biological properties, an active domain of these proteins has been predicted here.

关键词： Momorcharin ribosome-inactivating protein (RIP) primary structure sequence comparison

Purification and primary structure Determination of a Novel Polypeptide Isolated from Mistletoe Viscum coloratum

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2004年第11期15卷 1311-1314页

作者： JingLinKONG XiuBaoDU ChongXuFAN YingCAO HuiJIANG JianFuXU XiaoJunZHENG ResearchInstituteofPharmaceuticalChemistry Beijing102205

A novel polypeptide was isolated from mistletoe Viscum coloratum. The primary structure of the polypeptide named viscotoxin B2 was determined to be KSCCKNTTGRNIYNT CRFAGGSRERCAKLSGCKIISASTCPSDYPK by Edman degradation. Viscotoxin B2 shared high sequence homology with viscotoxins isolated from Viscum album. Pharmacological experiments showed that viscotoxin B2 had distinct cytotoxic activity on tumor cells. Viscotoxin B2 could be used as a leading compound in cancer therapy.

关键词： Viscum coloratum isolation primary structure viscotoxin.

Effect of RE Oxide on Impact Toughness and primary structure of Surfacing Metal

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research International 1998年第1期5卷 42-45页

作者： Yang Qingxiang Wang Airong Liao Bo Yao Mei Qi Junjie Yanshan University

The effect of rare earth(RE) oxide on impact toughness and primary structure of surfacing metal was investigated . The results show that the impact toughness of surfacing metal containing RE oxide can be increased by ... 详细信息

关键词： RE oxide impact toughness primary structure surfacing metal

THE primary structure OF ARROWHEAD PROTEINASE INHIBITOR

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 1991年第9期34卷 1070-1081页

作者：杨慧玲朱德煦戚正武 Department of Biochemistry Nanjing University Shanghai Institute of Biochemistry Academia Sinica

After the reduction and carboxymethylation of disulfide bonds, arrowhead proteinase inhibitors A and B were cleaved either by proteinases or by cyanogen bromide, the fractionated and purified peptides were then subjected to sequencing by a gas phase automatic sequencer, the primary structures were completed by the alignment of the peptides sequenced with overlapping peptides. Both inhibitors A and B consist of 150 amino acid residues with three pairs of disulfide bonds, share 90% homology in structure, and are markedly different from all other Ser proteinase inhibitors so far known. Hence, the arrowhead inhibitor may belong to a new inhibitor family. Based on their structure characteristics, it was deduced that both their two reactive sites might be located in the positions of Lys-Ser (45-46) and Arg-Tyr-Lys (77-79), respectively. Among 13 mutated residues in inhibitors A and B, the substitution of residue Arg in position 87 of inhibitor B for residue Leu in A might be the main cause of leading to difference in their inhibitory activities.

关键词： Ser proteinase inhibitor amino acid sequence arrowhead primary structure reactive site

A novel plant defensin from Chinese mistletoe,Viscum coloratum (Kom.) Nakai

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2007年第1期18卷 55-58页

作者： Shi Lei Liu Xiu Bao Du Jing Lin Kong Hui Jiang Research Institute of Chemical Defense Beijing 102205 China

A novel polypeptide named as defensin CM was isolated from Chinese mistletoe, Viscum coloratura （Kom.） Nakai. The amino acid sequence was determined by the combination of Edman degradation, endoproteinase Lys-C digestion, and MALDI-TOF mass spectrometry. The primary structure was ATCSAPSGRF KGACFSSNTC SNICKTLEGL KDGHCTGLAC YCSRNC.

关键词： Viscum coloratura Mistletoe primary structure Plant defensin Polypeptide

New Polypeptides from Chinese Mistletoe, Viscum coloratum (Kom.) Nakai

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2006年第1期17卷 41-44页

作者： Shi Lei LIU Xiu Bao DU Jing Lin KONG Research Institute of Chemical Defense Beijing 102205

Three viscotoxins have been isolated from Chinese mistletoe, Viscum coloratura （Kom.） Nakai. The primary structures were determined unambiguously by the combination of Edman degradation, endoproteinase Elu-c digestion, and Q-TOF mass spectrometry. One was identified as viscotoxin C1, which was for the first time isolated from Chinese mistletoe. The other two were new polypeptides, named as viscotoxin B5 and viscotoxin B8. Pharmacological studies showed that the polypeptides exhibit distinct cytotoxicity to human cancer cells.

关键词： Viscum coloratura mistletoe isolation primary structure viscotoxin.

Exploring the characteristics of lumbricus bimastus nucleases EWD1,2,3

在线全文

学校读者我要写书评

暂无评论

Exploring the characteristics of lumbricus bimastus nuclease...