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Determination of the prevalence of extended spectrum β-lactamase in clinical samples collected from Dehradun City Hospital

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Journal of Coastal Life Medicine 2016年第7期4卷 568-572页

作者： Narayan Sharma Ripan Mujumdar Rajeev Kumar Gautam Department of Pharmaceutical Chemistry Doon(PG)Paramedical College and HospitalDehradunUttarakhandIndia Department of Microbiology Doon(PG)Paramedical College and HospitalDehradunUttarakhandIndia

Objective:To detect extended spectrumβ-lactamase(ESBL)and determine its prevalence in various clinical samples collected from Dehradun City ***:The samples were first cultured in MacConkey’s agar plates by streak plate method,then identified by Gram staining and biochemical *** isolated bacterial strains were then tested for antibiotic susceptibility by Kirby-Bauer *** ESBL detection is then carried out by double disc diffusion ***:Off the 56 samples cultured,21 strains were identified which were six Escherichia coli(***),six Klebsiella,four Proteus,four Pseudomonas aeruginosa(***)and only one *** out of 21(38.1%)strains including three of ***,three of Klebsiella and two of ***,were found to be resistance to all five antibiotics(piperacillin,amikacin,ampicillin,gentamicin,and ciprofloxacin).Initial screening using four antibiotics(cefotaxime,ceftazidime,aztreonam and ceftriaxone)and the final confirmatory test using ceftazidime/clavulanic acid and ceftazidime alone showed that 19.05%of all strains isolated were ESBL ***,16.67%***,16.67%Klebsiella pneumoniae,25%*** and 100%Acinetobacter were found to be ESBL ***:Antibiotic resistance by ESBL has become a major risk factor worldwide,therefore routine checkup and accordingly prescription are suggested.

关键词： Antibiotic resistance Antimicrobial susceptibility test Double disc diffusion method prevalence of extended spectrum β-lactamase

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