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Determination of Content of Amino Acid in Abalone by Precolumn derivatization and its Nutritional Value Evaluation

Agricultural Biotechnology 2018年第3期7卷 178-183,210页

作者： Wen CHEN Xiangjun WANG Yujie WANG Yuzhi HUANG Fanmeng MENG Hui CHEN School of ocean science and technology Hainan Tropic Ocean University Sanya 572022 China Hainan Marine food engineering technology research center Sanya 572022 China School of life science and ecology Hainan Tropic Ocean University Sanya 572022 China

[Objectives] This study was conducted to optimize the determination conditions of amino acids from abalone. [Methods] The sample was treated by acid hydrolysis method and subjected to 2,4-2 nitro fluorobenzene column derivatization. The amino acid content in abalone was determined by HLPC,and the nutritional value of the amino acids was evaluated with egg protein model put forward by Institute of Nutrition and Food Hygiene,Chinese Academy of Preventive Medicine. [Results] Abalone contains full amino acids. According to the FAO/WHO ideal,it is a high-quality protein source and suitable for supplement of protein source for human body. [Conclusions]The experimental method has simple operation and could achieve a good effect with wide linear range and correlation coefficient over 0. 999 8,and the obtained results are satisfactory.

关键词： Abalone precolunm derivatization HPLC Amino acid Evaluation

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Study on Inspection of Synthetic Reaction for Preparation of N-(phosphonomethyl) iminodiacetic Acid with Iminodiacetic Acid by Precolumn derivatization-HPLC

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Agricultural Biotechnology 2017年第4期6卷 67-68,72页

作者： Yong ZHUANG Kai HUANG Nantong Changhai Food Additive Co. Ltd. Haimen 226121 China Fujian Key Laboratory of Screening of Novel Microbial Products Fujian Institute of Microbiology Fuzhou 350007 China

The derivatives of the reaction liquid for synthesizing N-（phosphonomethyl） iminodiacetic acid （PMIDA） were synthesized with 9-fluorenyl methyl chlomformate （FMOC-C1） as the derivatization reagent. The separation of the derivatives was performed on a Gemini C18 column at room temperature by isocratic elution. The mobile phase was composed of 60% 0.05 M Nail2 PO4 （pH = 3.5） and 40% CH3 CN at a flow rate of 1 ml/min. The injection volume was 10 td, and the detection wavelength was 265 nm. The linear ranges of the derivatives of iminodiacetonitrile, iminodiacetic acid （IDA） and ammonia were, respectively, 0.02-1.2, 0.02-1.2 and 0.1 -2. 0 mg/ml with correlation coefficients not smaller than 0. 999 8. The detection limits for the derivatives were, respectively, 0. 000 4, 0. 000 4 and 0.003 mg/ml. The average recoveries of 3 derivatives were respectively 99.96%, 99. 86% and 99.89%, respectively, with relative standard deviation values not smaller than 1.6%. The method developed in the present paper could be used for monitoring the reaction system for preparation of PMIDA by IDA.

关键词： Iminediacetic acid N- (phosphonomethyl) iminodiacetic acid High Perfornmnce Liquid Chromatography precolunm derivatization

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