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Effect of n-butanol Extract from potentilla anserina on Hypoxia-induced Calcium Overload and SERCA2 Expression of Rat Cardiomyocytes

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Chinese Herbal Medicines 2012年第2期4卷 142-149页

作者： Ling-zhi LI[a][b] Lu-jun WANG[b] Yue WANG[a][b] Ying CUI[a][b] Jian-yu LI[b] Li ZHANG[b] Yong-liang ZHANG[a][b] Tianjin Key Laboratory for B iomarkers of Occupational and Environmental Hazards Tianjin 300162 China Logistics University of Chinese People's Armed Police Forces Tianjin 300162 China Tianjin Key Laboratory for B iomarkers of Occupational and Environmental Hazards Tianjin 300162 China Logistics University of Chinese People's Armed Police Forces Tianjin 300162 China

Objective To investigate the effect of n-butanol extract from potentilla anserina(NP)intervention on hypoxia-induced Ca 2+ overload and SERCA2 expression of rat *** Primary cultured myocardial cell from SD neonatal rat(1-3 d)was used in the establishment of hypoxia *** hypoxia for 3 h,the Ca 2+ concentration of myocardial cells was measured with fura-2/AM fluorescent probe,and the biochemical indicator intracellular Ca 2+ -ATPase was examined and the mRNA and its protective protein levels of the sarcoplasmic reticulum(SR)Ca 2+ -ATPases(SERCA2)were assayed with RT-PCR,Western-blotting,and immune-cytochemical staining in each *** The results showed that NP decreased Ca 2+ concentration, increased the activity of Ca 2+ -ATPase,and improved the mRNA and protein expression of SERCA2 in hypoxia-injured myocardial cells as compared with the model *** These results indicate that NP could attenuate the Ca 2+ *** mechanism might be explained as that NP could elevate the SERCA2 level, increase the activity of myocardium in rats,and further enhance the capacity of SR Ca 2+ re-uptake.

关键词： cardiomyocyte hypoxia intracellular Ca 2+ potentilla anserina sarcoplasmic reticulum

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Neuroprotection of n-Butanol Extract from Roots of potentilla anserina on Hypoxic Injury in Primary Hippocampal Neurons

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Chinese Herbal Medicines 2012年第3期4卷 195-200页

作者： Xiao-jing QIN[a] Ling-zhi LI[b][c] Qi LV[d] Bao-guo YU[e] Shu-wang YANG[f] Tao HE[a] Yong-liang ZHANG[c][g] Department of Pathology Affiliated Hospital of Logistic University of Chinese People's Armed Police ForcesTianjin 300162China Department of Medicinal ChemistryLogistic University of Chinese People's Armed Police ForcesTianjin 300162China Tianjin Key Laboratory of Occupational and Environmental Hazard Biomarkers Tianjin 300162China Tianjin Key Laboratory of Occupational and Environmental Hazard BiomarkersTianjin 300162China Logistic University of Chinese People's Armed Police Forces Tianjin 300162China

Objective To investigate the protective effect of n-butanol extract from the roots of potentilla anserina (NP) on hypoxic hippocampal neurons in neonatal rats. Methods Primary cultured hippocampal neurons were pretreated with different concentration of NP (0.25, 0.0625, and 0.0156 mg/mL) before incubation in a low oxygen (0.1%) environment for 4 h. Cell viability was evaluated by Trypan blue staining assay. Lactate dehydrogenase (LDH) released by neurons into the medium was measured. The activity of superoxide dismutase (SOD) in cell cytosol was determined using nitroblue tetrazolium. Morphological changes and mitochondrial function were observed by transmission electron microscopy. Results Hypoxic injury could decrease the cells viability of neuron, enhance LDH release (P < 0.05), decrease SOD activity, and increase mitochondrial injury. Pretreatment with NP significantly increased cell viability, decreased LDH release (P < 0.05), promoted SOD activity (P < 0.05), and remarkably improved cellular ultra-microstructure compared with the model group. Conclusion NP could protect the primary hippocampal neurons from hypoxic injury by attenuating mitochondrial cell death.

关键词： hypoxia mitochondria neuron potentilla anserina ultra-microstructure

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