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中国植物病理学会2021年学术年会

作者： ZHAO Zhenxing MARTINS Guilherme Silva JIAO Zhiyuan BROSSEAU Chantal FAN Zaifeng MOFFETT Peter Department of Plant Pathology China Agricultural University Centre SèVE Département de BiologieUniversité de Sherbrooke Institute of Plant Inspection and Quarantine Chinese Academy of Inspection and Quarantine

RNA silencing is one of the important mechanisms of plant antiviral innate immunity（Calil and Fontes,2017;Li and Wang,2019）.Arabidopsis is usually not considered a host of PVx and is immune to its *** certain circumstances,PVx can break this non-host *** combined with another virus,PVx can infect Arabidopsis ecotype *** previous studies,it was shown that PVx can achieve systemic infection after inoculation in the dcl2/dcl3/dcl4 triple mutant,indicating that RNA silencing may be the cause of Arabidopsis immunity to PVx（Jaubert et al.,2011）.It has also been shown that the AtCol-0-AGO2 allele confers resistance to PVx,whereas the AG02 allele from the C24 ecotype（AtC24-AG02） does *** reason for this difference may be the longer RGR repeat motif and D33 sites located in the N-terminus of *** variants are not located in the known functional domain of AG02（Brosseau et al.,2019）.The molecular role of these two sites involved in antiviral resistance is still *** study aims to use the tobacco leaf transient overexpression system and transgenic overexpression Arabidopsis lines to explore the function of the Nterminal region of the AGO2 protein and its effect on the relationship between PVx and P25.

关键词： RNA silencing ARGONAUTE 2 viral suppressor of RNA silencing potato virus x

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Effects of Synergism Between PVY and PVx on virus Titer and Cell Ultrastructure in Tobacco Plants

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Agricultural Sciences in China 2003年第2期2卷 163-169,T001-T002页

作者： LI Han , GUO xing-qi , LI xiang-dong, GUO Heng-jun and LI Zhao-huiCollege of Life Sciences , Shandong Agricultural University ,Tai’an 271018 , P. R. China College of Plant Protection , Shandong Agricultural University , Tai’an 271018 , P. R. China College of Life Sciences Shandong Agricultural University Tai'an 271018 P. R. China College of Plant Protection Shandong Agricultural University Tai'an 271018 P. R. China

The viruses titer and the ultrastructure of infected cells in tobacco host (Nicotiana tabacum cv. Samsun), which doubly infected with potato virus Y necrosis strain (PVYN) and potato virus x (PVx), were studied under greenhouse conditions. The results indicated that PVYN and PVx interacted synergistically. and tobacco plants which doubly infected with PVx and PVYN could greatly increase symptom severity as compared with that induced by the single virus. As determined by triple antibody sandwich enzyme-linked immunosor-bent assay (TAS-ELISA), the titer of PVx in the tobacco leaves infected with both PVYN and PVx was up to 9.10 times higher than the plants infected with PVx only. No significant differences in PVYN titer were detected between singly and doubly infected plants. The enhancement of PVx titer in doubly infected plants was evident in greenhouse and was independent of the virus strains, tested seasons, intervals between PVYN and PVx inoculation. When sections of doubly infected leaves were examined with an electron microscope, it could be frequently found that cells contained pinwheels and large masses of PVx-like particles, pinwheels and laminate inclusions, or all three structures, most of them were swollen, and their chloroplast and other organella were destructed heavily. This suggested that doubly infected cells were involved in the enhancement phenomenon, which seemed to be the result of an increase in the amount of PVx synthesized in them.

关键词： potato virus Y potato virus x Synergism Cytopathology

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Evaluation of potential RNA-interference-target genes to control cotton mealybug, Phenacoccus solenopsis （Hemiptera： Pseudococcuidae）

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Insect Science 2018年第5期25卷 778-786页

作者： Arif M. Khan Muhammad Ashfaq Azhar A. Khan Muhammad T. Naseem Shahid Mansoor National Institute for Biotechnology and Genetic Engineering Faisalabad Pakistan Department of Biotechnology University of Sargodha Sargodha Pakistan Biodiversity Institute of Ontario University of Guelph Guelph Ontario Canada College of Agriculture Bahauddin Zakariya University Bahadur Campus Layyah Pakistan

RNA interference （RNAi） of vital insect genes is a potential tool for targeted pest control. However, selection of the right target genes is a challenge because the RNAi efficacy is known to vary among insect species. Cotton mealybug, Phenacoccus solenopsis, is a phloem-feeding economically important crop pest. We evaluated the RNAi of 2 vital genes, Bursicon （PsBur） and V-ATPase （Ps V-ATPase） as potential targets in P. solenopsis for its control. PCR fragments of PsBur and PsV-ATPase were amplified using cDNA synthesized from the total RNA. The PCR amplicons were cloned into potato virus x （PVx） to develop recombinant PVx for the inoculation ofNicotiana tabacum plants for bioassays with healthy P. solenopsis. Reverse-transcription-polymerase chain reaction （RT- PCR） was used to validate the expression oftransgenes in the recombinant-PVx-inoculated plants （treated）, and suppression of the target genes in the mealybugs exposed to them. The RT-PCR confirmed the expression of transgenes in the treated plants. Mealybug individuals on treated plants either died or showed physical deformities. Further, the population of mealybug was significantly reduced by feeding on N. tabacum expressing RNAi triggers against PsBur and Ps V-A TPase. The results conclude that RNAi is activated in P. solenopsis by feeding on N. tabacum expressing RNAi triggering elements of PsBur and Ps V-ATPase genes through recombinant PVx vector. Further, V-ATPase and Bursicon genes are potential targets for RNAi-mediated control ofP. solenopsis.

关键词： insect control phloem feeders potato virus x RT-PCR transgenic crops

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