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Plant Proteomics in the post-genomic Era

Acta Botanica Sinica 2002年第6期44卷 631-641页

作者：郭奕明沈世华荆玉祥匡廷云中国科学院植物研究所北京100093

Proteomics is one of the most active research fields in the post-genomic era. Here we briefly introduce the scientific background of the origination of proteomics and its content, research method. The new developments of proteomics at the levels of individual plants, tissues, organs and organells, as well as its applications in the area of plant genetic diversity, mutant characterization, and plant physiology, etc are reviewed. At last, the challenge and prospect of proteomics are discussed.

关键词： genome post-genome proteome plant proteomics matrix-assisted laser-desorption/ionizationtime-of-flight mass spectrometry (MALDI-TOF/MS) two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)

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Insulin-secreting β cells require a post-genomic concept

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World Journal of Diabetes 2016年第10期7卷 198-208页

作者： Fang-Xu Jiang Grant Morahan Islet Cell Development Program Harry Perkins Institute of Medical Research Nedlands WA 6009 Australia Centre for Diabetes Research the University of Western Australia Perth WA 6009 Australia

Pancreatic insulin-secretingβcells are essential in maintaining normal glucose homeostasis accomplished byhighly specialized transcription of insulin gene,of which occupies up to 40%their *** of these cells causes diabetes mellitus,a global public health *** tremendous endeavors have been made to generate insulin-secreting cells from human pluripotent stem cells(i.e.,primitive cells capable of giving rise to all cell types in the body),a regenerative therapy to diabetes has not yet been ***,the nomenclature ofβcells has become inconsistent,confusing and controversial due to the lack of standardized positive controls of developmental stagematched in vivo *** order to minimize this negative impact and facilitate critical research in this field,a postgenomic concept of pancreaticβcells might be *** this review article,we will briefly describe howβcells were discovered and islet lineage is developed that may help understand the cause of nomenclatural controversy,suggest a post-genomic definition and finally provide a conclusive remark on future research of this pivotal cell.

关键词： Beta cells Insulin post-genome Concept

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Development of Studies on RNA Interference

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Agricultural Science & Technology 2012年第9期13卷 1838-1842页

作者：张俐张雅琼佘丽娜徐文婷贾阳映谢世清孙文丽梁泉云南省科学技术情报研究院云南昆明650051 云南中医学院云南昆明650500 云南农业大学农学与生物技术学院云南昆明650201

RNA interference （RNAi）, caused by endogenous or exogenous double- stranded RNA （dsRNA） homologous with target genes, refers to gene silencing widely existing in animals and plants. It was first found in plants, and now it has developed into a kind of biotechnology as well as an important approach in post- genome era. This paper is to summarize the achievements of studies on RNAi tech- nology in basic biology, medicine, pharmacy, botany and other fields.

关键词： RNA interference post-genome Functional genome

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