检索结果-南通市图书馆

ILipo-PseAAC: Identification of Lipoylation Sites Using Statistical Moments and General PseAAC

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Computers, Materials & Continua 2022年第4期71卷 215-230页

作者： Talha Imtiaz Baig Yaser Daanial Khan Talha Mahboob Alam Bharat Biswal Hanan Aljuaid Durdana Qaiser Gillani School of Science and Technology University of Management and TechnologyLahorePakistan Department of Computer Science and Information Technology Virtual University of PakistanLahorePakistan Department of Biomedical Engineering New Jersey Institute of TechnologyNewarkNJUSA Department of Computer Sciences College of Computer and Information SciencesPrincess Nourah bint Abdul Rahman University(PNU)RiyadhSaudi Arabia Department of Economics University of LahoreLahorePakistan

Lysine Lipoylation is a protective and conserved post translational modification(PTM)in proteomics research like prokaryotes and eukaryotes.It is connected with many biological processes and closely linked with many metabolic diseases.To develop a perfect and accurate classification model for identifying lipoylation sites at the protein level,the computational methods and several other factors play a key role in this purpose.Usually,most of the techniques and different traditional experimental models have a very high cost.They are time-consuming;so,it is required to construct a predictor model to extract lysine lipoylation sites.This study proposes a model that could predict lysine lipoylation sites with the help of a classification method known as Artificial Neural Network(ANN).The ANN algorithm deals with the noise problem and imbalance classification in lipoylation sites dataset samples.As the result shows in ten-fold cross-validation,a brilliant performance is achieved through the predictor model with an accuracy of 99.88%,and also achieved 0.9976 as the highest value of MCC.So,the predictor model is a very useful and helpful tool for lipoylation sites prediction.Some of the residues around lysine lipoylation sites play a vital part in prediction,as demonstrated during feature analysis.The wonderful results reported through the evaluation and prediction of this model can provide an informative and relative explanation for lipoylation and its molecular mechanisms.

关键词： Lipoylation lysine feature vector post translational modification amino acid Mathew’s correlation coefficient neural network

Comprehensive Profiling for Histone H4 of Human Liver Cells Using High Resolution LTQ-Orbitrap Mass Spectrometry

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2011年第1期29卷 171-177页

作者： Nie, Aiying LU, Haojie Yang, Pengyuan He, Fuchu Department of Chemistry and Institute of Biomedical Sciences Fudan University Shanghai 200433 China State Key Laboratory ofProteomics Beijing Proteome Research Center Beijing Institute of Radiation Medicine Beijing 102206 China

The post translational modifications of histone variants are playing an important role in the structure of chromatin, the regulation of gene activities and the diagnosis of diseases, and conducting in-depth researches and discovering new sites depend on new and rational analytical methods to some extent. In this work, the combinatorial method of high resolution LTQ-Orbitrap mass spectrometry and multiple enzymes was employed to identify the post translational modifications （PTMs） of histone H4 of human liver cells. The novel methylation site, argnine 67 （R 67）, was observed besides some sites reported previously such as lysine 31 （K 31）, lysine 44 （K 44）, argnine 55 （R 55） and lysine 59 （K 59） in the global domain. Meanwhile, various combinations of acetylation of lysine 5 （K 5）, lysine 8 （K 8）, lysine 12 （K 12）, lysine 16 （K 16） and methylation of lysine 20 （K 20） in the NH2-terminal tails were also identified after the LC-MS/MS analysis of trypsin, Arg-C, Glu-C and chymotrypsin digests.

关键词： histone post translational modification mass spectrometry enzymes

Bmi-1:At the crossroads of physiological and pathological biology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2015年第3期2卷 225-239页

作者： Resham Bhattacharya Soumyajit Banerjee Mustafi Mark Street Anindya Dey Shailendra Kumar Dhar Dwivedi Department of Obstetrics and Gynecology Stephenson Cancer CenterUniversity of Oklahoma Health Science CenterOklahoma CityOK 73104United States of America

Bmi-1 is a member of the Polycomb repressor complex 1 that mediates gene silencing by regulating chromatin structure and is indispensable for self-renewal of both normal and cancer stem cells.Despite three decades of research that have elucidated the transcriptional regulation,post-translational modifications and functions of Bmi-1 in regulating the DNA damage response,cellular bioenergetics,and pathologies,the entire potential of a protein with such varied functions remains to be realized.This review attempts to synthesize the current knowledge on Bmi-1 with an emphasis on its role in both normal physiology and cancer.Additionally,since cancer stem cells are emerging as a new paradigm for therapy resistance,the role of Bmi-1 in this perspective is also highlighted.The wide spectrum of malignancies that implicate Bmi-1 as a signature for stemness and oncogenesis also make it a suitable candidate for therapy.Nonetheless,new approaches are vitally needed to further characterize physiological roles of Bmi-1 with the long-term goal of using Bmi-1 as a prognostic marker and a therapeutic target.

关键词： Aging Bmi-1 Cancer post translational modification Stem cell

Biochemical analysis of granzyme H and elucidation of its structurally important residues involved in substrate and inhibitor binding

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Chinese Pharmaceutical Sciences 2012年第1期21卷 33-39页

作者： Naheed Z Razwi Rukhshan Khurshid Mahjabeen Saleem Sabiha Karim Saeed Ahmad Nagra Asmat Salim Department of Biochemistry CMH Lahore Medical CollegeLahorePakistan Fatima Jinnah Medical College LahorePakistan Institute of Biochemistry and Biotechnology University of the PunjabLahorePakistan College of Pharmacy University of the PunjabLahore Institute of Chemistry University of the PunjabLahorePakistan Dr.Panjwani Center for Molecular Medicine and DrugResearch International Center for Chemical and Biological SciencesKarachi UniversityPakistan

Apoptotic cell death plays an important role in the maintenance of the normal physiological state and in the pathogenesis of diseases.Granule exocytosis is the main pathway for the immune elimination of virus-infected cells and tumor cells by cytotoxic T lymphocytes and natural killer cells.In recent study,we have investigated the level of granzyme H in patients with breast cancer and in control subjects using enzymatic method.Our study also included the prediction of different sites of granzyme H that play a role in substrate and inhibitor recognition in apoptosis process by using 3D structural model of the enzyme.The research described the possible post-translational modification sites that may help the enzyme in immune elimination of tumor cells.Our study shows that the level of granzyme H was reduced in patients when compared to normal control subjects.There are a number of amino acids that function as substrate recognition sites in granzyme H.However,inhibitors may inhibit their activity and affect the process of autolysis.

关键词： Autolysis Granzyme H Apoptosis post translational modification

First Crotonyl-proteome Profiling and Genome-wide Mapping Reveals the Involvement of crotonylation in Oryza sativa

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

First Crotonyl-proteome Profiling and Genome-wide Mapping Re...