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porcine parvovirus Inducing Autophagy to Benefit Its Replication

Journal of Northeast Agricultural University(English Edition) 2018年第4期25卷 43-52页

作者： Zhang Yue Li Xiao-xue Zhong Ming Chen Hui-jie Huang Xiao-dan Bai Yun-yun Cong Ying-ying Zhang Rui-li Li Guang-xing Key Laboratory for Laboratory Animals and Comparative Medicine of Heilongjiang Province College of Veterinary Medicine Northeast Agricultural University College of Pharmaceutical Engineering Jilin Agriculture Science and Technology College

porcine parvovirus(PPV) is one of the major causes of reproductive failure in pigs, which poses a great threat to the pig breeding industry and results in tremendous economic losses worldwide. Autophagy is the biological process of cell self-defense and self-protection. Despite many viruses can cause cell autophagy, when they enter cell or copied, the relationship between autophagy and PPV infection has not been reported. In this study, impact of autophagy after swine testicular(ST) cells infected by PPV was studied. Autophagy was demonstrated by the effective replication of PPV through transmission electron microscopy, immunofluorescence and western blot analysis. Moreover, autophagy was confirmed to benefit PPV replication by real-time fluorescence quantitative PCR and determination of median tissue culture infective dose(TCID). For the first time, the complex interaction between PPV infection and autophagy was explored in this study. It indicated that PPV could induce autophagy in ST cells, which in turn facilitated its own replication, which might be one of the mechanisms of the virus infection. These findings could facilitate the study of the pathogenesis of PPV infection and provide new insight into the development of effective therapeutic strategies.

关键词： autophagy porcine parvovirus virus replication apoptosis

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Epidemiology Survey of porcine parvovirus and porcine Circovirus Type 2 in Sichuan Province

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Animal Husbandry and Feed Science 2012年第3期4卷 130-132页

作者： XU Jun GUO Wan-zhu CHEN Yang XU Zhi-wen WANG Xiao-yu Chengdu Vocational College of Agricultural Science and Technology Animal Biotechnology Center of Sichuan Agricultural University Chengdu Chia Tai Co LTD

[ Objective] The study aimed to analyze the prevalence of porcine parvovirus （PPV） and porcine Circovirus Type 2 （ PCV2 ） and the mixed infection in Sichuan Province to lay the foundation for further predicting the tendency of the plague and formulating appropriate prevention and control strategies. [ Method] Two hundred and seventy -three samples were collected from 21 large pig farms in Sichuan province, and epidemiology of PPV and PCV2 were investigated by PCR detecting. [ Result] The positive rate of PPV was 17.22% （47/273）, and positive rate of pig farms was 38.1% （8/21）, meanwhile it also displayed the feature that infection rate of boar was higher than that of piglets; The positive rate of PCV2 was 52.38% （143/273）, and positive rate of pig farms was 85.7% （18/21）, and it showed the trend that the infection rate of PCV2 was rising with the growth of the age. The co-infection rate of PPV and PCV2 was 10.62% （29/273）, and co-infection rate of pig farms was 28.7% （6/21）, which mainly concentrated in the sow and nursery piglets. Only 14.3% （3/21） pig farms was epidemiologically negative of PPV and PCV2. [ Conclusion] PPV and PCV2 and co-infection was widely popular in Sichuan province, and it did more serious harm to the pig-raising industry.

关键词： porcine parvovirus porcine circovirus 2 epidemiology

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Molecular Cloning and Prokaryotic Expression of Non-Structural Protein NS1 Gene of porcine parvovirus

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Agricultural Sciences in China 2003年第10期2卷 1175-1178页

作者： WUDan TONGGuang-zhi QIUHua-ji XUEQiang ZHOUYan-jun LIJing-peng NationalKeyLaboratoryofVeterinaryBiotechnology HarbinVeterinaryResearchInstituteHarbin150001P.R.China DepartmentofBiotechnology NortheastAgriculturalUniversityHarbin150030P.R.China

porcine parvovirus (PPV) is one of the major agents causing swine reproductive failure. NS1 protein is a non-structural protein of PPV and can be used as a reagent for differentiation of vaccinated animals and infected ones. In present study, a recombinant plasmid pET28a/NS1 was constructed by cloning the coding sequence for NS1 of PPV into pET28a, a bacterial expression vector. The NS1 protein was expressed in E. coli BL21(DE3) after induced by IPTG and the recombinant fusion protein was purified with affinity chromatography. Expression amount of NS1 protein was improved by optimizing the inducing parameters. The recombinant NS1 protein is reactive to PPV positive sera in Western blot and ELISA test and therefore can be applicable in differential diagnosis of PPV infections.

关键词： porcine parvovirus NS1 Diagnosis

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重组口蹄疫病毒T细胞表位基因的猪细小病毒VP2蛋白病毒样颗粒的构建

重组口蹄疫病毒T细胞表位基因的猪细小病毒VP2蛋白病毒样颗粒的构...

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中国畜牧兽医学会兽医公共卫生学分会第二次学术研讨会

作者：潘群兴王永山何孔旺欧阳伟王晓丽诸玉梅夏兴霞江苏省农业科学院兽医研究所农业部动物疫病诊断与免疫重点开放实验室国家兽用生物制品工程技术研究中心

将O型口蹄疫病毒（FMDV）VP1上T细胞表位肽（第21～40残基）基因嵌合在PPV VP2的N端,克隆到杆状病毒供体质粒pFastBac HTA中,构建成重组转座载体pFastBac-FMDV VP1:PPV VP2,转化含有穿梭载体Bacmid的E.coliDH10Bac中,发生转座作用,经蓝... 详细信息

将O型口蹄疫病毒（FMDV）VP1上T细胞表位肽（第21～40残基）基因嵌合在PPV VP2的N端,克隆到杆状病毒供体质粒pFastBac HTA中,构建成重组转座载体pFastBac-FMDV VP1:PPV VP2,转化含有穿梭载体Bacmid的E.coliDH10Bac中,发生转座作用,经蓝白斑筛选获得重组杆状病毒表达质粒rBac-FMDV VP1:PPVVP2,转染昆虫草地夜蛾（Sf9）细胞,并在Sf9中进行了表达。用间接免疫荧光试验（IFA）、Westem-blot分析,表达产物具有与PPV VP2天然蛋白相似的生物活性。电镜观察,表达的蛋白能够自我组装成病毒样粒子[PPV:VLP（FMDV）]。

关键词： porcine parvovirus Food-and-mouth disease virus type 0 Virus-like particles

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