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Prevalence of porcine endogenous retrovirus in Chinese pig breeds and in patients treated with a porcine liver cell-based bioreactor

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World Journal of Gastroenterology 2005年第30期11卷 4727-4730页

作者： Qing Liu Zheng Liu Evangelos Dalakas Artificial Liver Treatment and Training Center Beijing Youan Hospital Capital University of Medical Sciences Beijing 100054 China Department of General Surgery Shen Zheng Hospital Peking University Shenzheng 518036 Guangdong Province China Department of Hepatology Chancellor's Building University of Edinburgh Edinburgh EH16 4SB Scotland UK

AIM： To determine the prevalence of porcine endogenous retrovirus （PERV） in various pig breeds raised in China including Chinese experimental mini-pigs by PERV-reverse transcriptase （PERV-RT enzyme）. Moreover, the potential for infection of PERV was investigated in patients treated with a bioreactor based on porcine liver cells （n = 3）. METHODS： Pig serum, liver and muscle cell-free supernatants were collected from various Chinese pig breeds. porcine hepatocytes were isolated with a two-step perfusion method. Three patients with acute or chronic liver failure were treated with a bioartificial liver support system （BALSS） for 8-12 h and serum samples were collected from the patients before, immediately after and 30 d after treatment. RESULTS： The activities of PERV-RT enzyme in pig liver and muscle cell-free supernatants were higher than in normal human controls. PERV-TR enzyme activity did not increase in patients before and after 1 mo of treatment. PERV-RT activities were not significantly different when compared with pre-treatment group （1.544±0.155576）, the post-treatment groups （1.501±0.053507, 1.461±0.033808 and 1.6006667±0.01963 for 0, 14 and 30 d post-treatment, respectively, P〉0.05）, and normal control group （1.440±1.0641, P〉0.05）. RT enzyme activity in Chinese experimental mini-pigs was higher than in normal human control group （1.440±1.0641 U/mL, P〈0.05）, and not significantly different （P〉0.05） when compared with the pig breeds except in the muscle supernatants. All the samples including muscle and liver cell supernatants from the Chinese mini-experimental pigs and the four domestic Chinese pig breeds contained PERVs. CONCLUSION： These results suggest that the risk of PERV infection through BALSS containing porcine liver cells without immunosuppressants may be quite low. Although there were PERVs in Chinese experimental mini-pigs and porcine liver cell culture suspensions, we did not find any evidence of persistent PERV infection in patients treated with

关键词： porcine endogenous retrovirus Bioartificial liver support system porcine endogenous retrovirus-reverse transcriptase

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Ancient origin and complex evolution of porcine endogenous retroviruses

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Biosafety and Health 2020年第3期2卷 142-151页

作者： Yicong Chen Mingyue Chen Xiaoyan Duan Jie Cui Wuhan Institute of Virology Center for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China CAS Key Laboratory of Molecular Virology and Immunology Institute Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China National 111 Center for Cellular Regulation and Molecular Pharmaceutics Key Laboratory of Fermentation EngineeringHubei University of TechnologyWuhan 430068China

porcine endogenous retroviruses(PERVs)are proviruses that have medical value in ***,the evolutionary process leading to the generation of modern PERVs is not well *** this study,we in silico mined 14 pig genomes and other available 304 mammalian genomes,which led to the documentation of 185 full-length PERVs or PERV-like ***,we found that lesser Egyptian jerboa(Jaculus jaculus),rock hyrax(Procavia capensis)and eight Muridae species all harbored ancestral PERV-like sequences,indicative of ancient cross-species transmission events from none-porcine species to *** also revealed that 11 genomic rearrangement events were mediated via PERVs,during the process of porcine *** conclusion,these new findings help us to understand the complex evolution of the modern PERVs.

关键词： porcine endogenous retrovirus Evolution Origin Genomic rearrangement Cross-species transmission

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Influence of chitosan nanofiber scaffold on porcine endogenous retroviral expression and infectivity in pig hepatocytes

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World Journal of Gastroenterology 2011年第22期17卷 2774-2780页

作者： Bing Han Xiao-Lei Shi Jiang-Qiang Xiao Yue Zhang Xue-Hui Chu Jin-Yang Gu Jia-Jun Tan Zhong-Ze Gu Yi-Tao Ding Department of Hepatobiliary Surgery the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008Jiangsu ProvinceChina Department of Hepatobiliary Surgery Drum Tower Clinical Medical College of Nanjing Medical UniversityNanjing 210008Jiangsu ProvinceChina State Key Laboratory of Bioelectronics Southeast UniversityNanjing 210000Jiangsu ProvinceChina

AIM: To investigate the influence of chitosan nanofiber scaffold on the production and infectivity of porcine endogenous retrovirus (PERV) expressed by porcine hepatocytes. METHODS: Freshly isolated porcine hepatocytes were cultured with or without chitosan nanofiber scaffold (defined as Nano group and Hep group) for 7 d. The daily collection of culture medium was used to detect reverse transcriptase (RT) activity with RT activity assaykits and PERV RNA by reverse transcription-polymerase chain reaction (PCR) and real time PCR with the PERV specific primers. And Western blotting was performed with the lysates of daily retrieved cells to determine the PERV protein gag p30. Besides, the in-vitro infectivity of the supernatant was tested by incubating the human embryo kidney 293 (HEK293) cells. RESULTS: The similar changing trends between two groups were observed in real time PCR, RT activity assay and Western blotting. Two peaks of PERV expression at 10H and Day 2 were found and followed by a regular decline. No significant difference was found between two groups except the significantly high level of PERV RNA at Day 6 and PERV protein at Day 5 in Nano group than that in Hep group. And in the in-vitro infection experiment, no HEK293 cell was infected by the supernatant. CONCLUSION: Chitosan nanofiber scaffold might prolong the PERV secreting time in pig hepatocytes but would not obviously influence its productive amount and infectivity, so it could be applied in the bioartificial liver without the increased risk of the virus transmission.

关键词： Chitosan nanofiber scaffold porcine hepatocyte porcine endogenous retrovirus Bioartificial liver

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